NKRF (NM_001173487) Human Tagged ORF Clone

CAT#: RC230446

NKRF (Myc-DDK-tagged)-Human NFKB repressing factor (NKRF), transcript variant 1

Product Images

Circular map for RC230446

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NKRF
• Synonyms: ITBA4; NRF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230446 representing NM_001173487
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTTTATGTTACCTCTCATCTTCAGGTACAGCCCACGCTTGATGGAAAAAATTCTCCAAATGGCTG
  AAGGTATTGATATTGGGGAGATGCCTTCATATGATCTGGTGCTGTCCAAACCTTCCAAAGGTCAAAAACG
  CCACCTCTCAACATGTGATGGTCAAAATCCTCCTAAAAAGCAAGCCGGTTCCAAATTCCATGCGAGACCT
  CGTTTTGAGCCTGTACATTTTGTAGCTAGTAGTTCAAAAGATGAAAGACAGGAAGATCCTTATGGCCCTC
  AAACAAAAGAGGTAAATGAACAAACACATTTTGCCAGCATGCCAAGAGACATCTACCAAGATTATACTCA
  AGACTCTTTCAGTATACAAGATGGGAATTCTCAGTATTGTGATTCATCAGGATTCATTCTCACAAAAGAC
  CAGCCTGTAACAGCCAACATGTATTTTGACAGTGGGAACCCTGCCCCAAGCACCACATCACAGCAGGCAA
  ACTCTCAGTCAACTCCTGAGCCTTCACCATCACAGACATTTCCCGAGTCTGTGGTAGCCGAGAAGCAGTA
  TTTTATTGAAAAATTAACGGCGACAATCTGGAAGAACCTTTCTAATCCAGAAATGACTTCTGGATCTGAT
  AAAATTAATTATACATATATGTTAACTCGTTGTATTCAGGCGTGTAAGACAAATCCTGAGTATATATATG
  CTCCTTTAAAGGAAATTCCTCCTGCCGACATCCCCAAAAATAAAAAACTTCTAACTGATGGCTATGCTTG
  TGAAGTTAGATGCCAAAATATCTACTTAACTACAGGTTATGCTGGCAGCAAGAATGGGTCCAGGGATCGA
  GCTACAGAGCTAGCTGTAAAACTCTTGCAGAAACGTATTGAAGTTAGAGTTGTCCGGCGGAAATTCAAGC
  ATACATTTGGAGAGGACCTCGTGGTGTGTCAGATTGGCATGTCCTCCTATGAATTTCCTCCAGCTCTGAA
  GCCACCAGAAGACCTGGTGGTGCTGGGTAAAGATGCTTCCGGGCAGCCAATTTTTAATGCTTCTGCCAAA
  CACTGGACCAATTTTGTCATTACAGAAAATGCAAATGATGCAATTGGTATCCTTAACAATTCTGCCTCAT
  TCAACAAGATGTCAATTGAATACAAATATGAGATGATGCCAAATCGCACATGGCGTTGTCGAGTGTTTTT
  ACAAGATCACTGCTTAGCTGAAGGTTATGGAACCAAGAAAACAAGTAAACATGCAGCTGCCGACGAGGCT
  TTGAAAATTCTTCAAAAAACACAGCCCACTTATCCATCTGTCAAAAGTTCACAATGCCATACAGGCTCTT
  CACCCAGAGGATCTGGAAAGAAGAAAGATATAAAGGATCTTGTAGTTTATGAGAATTCTTCAAATCCCGT
  GTGCACGCTGAACGACACAGCTCAGTTTAACCGAATGACAGTTGAGTATGTCTATGAAAGGATGACAGGC
  CTCCGCTGGAAATGCAAAGTGATTCTAGAGAGTGAAGTAATTGCAGAAGCAGTTGGGGTGAAGAAAACTG
  TCAAATATGAAGCTGCTGGGGAAGCTGTGAAAACCCTCAAAAAGACCCAGCCAACTGTCATTAACAACTT
  GAAGAAAGGAGCTGTTGAAGATGTGATTTCAAGAAATGAAATTCAGGGCCGCTCAGCAGAGGAGGCTTAC
  AAACAGCAAATCAAAGAAGATAATATTGGAAATCAGCTGCTGAGAAAGATGGGTTGGACTGGTGGTGGTT
  TAGGTAAATCTGGTGAGGGCATACGGGAGCCTATCTCAGTGAAAGAGCAGCATAAGCGGGAAGGGCTTGG
  TCTGGATGTAGAGAGGGTGAATAAAATTGCCAAGAGAGATATTGAACAGATCATCAGAAACTACGCCCGC
  TCCGAGAGCCACACAGATTTGACTTTCTCTAGAGAGCTGACTAATGATGAACGGAAGCAAATACATCAGA
  TTGCCCAGAAGTATGGTCTTAAGAGTAAGTCTCATGGGGTGGGCCATGATAGGTACCTAGTGGTAGGTAG
  AAAAAGACGGAAGGAAGACCTACTAGATCAGCTCAAACAGGAAGGCCAAGTGGGCCATTACGAGCTTGTT
  ATGCCTCAAGCAAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230446 representing NM_001173487
  Red=Cloning site Green=Tags(s)
  
  MGFMLPLIFRYSPRLMEKILQMAEGIDIGEMPSYDLVLSKPSKGQKRHLSTCDGQNPPKKQAGSKFHARP
  RFEPVHFVASSSKDERQEDPYGPQTKEVNEQTHFASMPRDIYQDYTQDSFSIQDGNSQYCDSSGFILTKD
  QPVTANMYFDSGNPAPSTTSQQANSQSTPEPSPSQTFPESVVAEKQYFIEKLTATIWKNLSNPEMTSGSD
  KINYTYMLTRCIQACKTNPEYIYAPLKEIPPADIPKNKKLLTDGYACEVRCQNIYLTTGYAGSKNGSRDR
  ATELAVKLLQKRIEVRVVRRKFKHTFGEDLVVCQIGMSSYEFPPALKPPEDLVVLGKDASGQPIFNASAK
  HWTNFVITENANDAIGILNNSASFNKMSIEYKYEMMPNRTWRCRVFLQDHCLAEGYGTKKTSKHAAADEA
  LKILQKTQPTYPSVKSSQCHTGSSPRGSGKKKDIKDLVVYENSSNPVCTLNDTAQFNRMTVEYVYERMTG
  LRWKCKVILESEVIAEAVGVKKTVKYEAAGEAVKTLKKTQPTVINNLKKGAVEDVISRNEIQGRSAEEAY
  KQQIKEDNIGNQLLRKMGWTGGGLGKSGEGIREPISVKEQHKREGLGLDVERVNKIAKRDIEQIIRNYAR
  SESHTDLTFSRELTNDERKQIHQIAQKYGLKSKSHGVGHDRYLVVGRKRRKEDLLDQLKQEGQVGHYELV
  MPQAN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001173487
• ORF Size: 2115 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001173487.1, NP_001166958.1
• RefSeq ORF: 2118 bp
• Locus ID: 55922
• UniProt ID: O15226
• Cytogenetics: Xq24
• Protein Families: Transcription Factors
• MW: 79.9 kDa
• Gene Summary: This gene encodes a transcriptional repressor that interacts with specific negative regulatory elements to mediate transcriptional repression of certain nuclear factor kappa B responsive genes. The protein localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]