NKRF (NM_001173487) Human Tagged ORF Clone

TrueORF^®
TrueORF^®

Expression-ready ORF plasmid with C-terminal tag(s)

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SKU

RC230446

NKRF (Myc-DDK-tagged)-Human NFKB repressing factor (NKRF), transcript variant 1

Datasheet SDS Plasmid Map

Reconstitution Protocol

NM_001173487 in other vectors (4)

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$710.00

3 Weeks*

Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA806097	NKRF mouse monoclonal antibody, clone OTI4C2 (formerly 4C2)	100 ul	$447.00
TA592569S	DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags	30 ul	$200.00
CC100003	DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)	5 x 200 ul	$160.00
PRIMER	Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each	100 pmol	$59.00
PS100001	pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug	10 ug	$650.00
AR100028M	DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL	5 ml	$720.00

Specifications

Specifications
Product Data
Type	Human Tagged ORF Clone
Target Symbol	NKRF
Synonyms	ITBA4; NRF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence >RC230446 representing NM_001173487 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTTTATGTTACCTCTCATCTTCAGGTACAGCCCACGCTTGATGGAAAAAATTCTCCAAATGGCTG AAGGTATTGATATTGGGGAGATGCCTTCATATGATCTGGTGCTGTCCAAACCTTCCAAAGGTCAAAAACG CCACCTCTCAACATGTGATGGTCAAAATCCTCCTAAAAAGCAAGCCGGTTCCAAATTCCATGCGAGACCT CGTTTTGAGCCTGTACATTTTGTAGCTAGTAGTTCAAAAGATGAAAGACAGGAAGATCCTTATGGCCCTC AAACAAAAGAGGTAAATGAACAAACACATTTTGCCAGCATGCCAAGAGACATCTACCAAGATTATACTCA AGACTCTTTCAGTATACAAGATGGGAATTCTCAGTATTGTGATTCATCAGGATTCATTCTCACAAAAGAC CAGCCTGTAACAGCCAACATGTATTTTGACAGTGGGAACCCTGCCCCAAGCACCACATCACAGCAGGCAA ACTCTCAGTCAACTCCTGAGCCTTCACCATCACAGACATTTCCCGAGTCTGTGGTAGCCGAGAAGCAGTA TTTTATTGAAAAATTAACGGCGACAATCTGGAAGAACCTTTCTAATCCAGAAATGACTTCTGGATCTGAT AAAATTAATTATACATATATGTTAACTCGTTGTATTCAGGCGTGTAAGACAAATCCTGAGTATATATATG CTCCTTTAAAGGAAATTCCTCCTGCCGACATCCCCAAAAATAAAAAACTTCTAACTGATGGCTATGCTTG TGAAGTTAGATGCCAAAATATCTACTTAACTACAGGTTATGCTGGCAGCAAGAATGGGTCCAGGGATCGA GCTACAGAGCTAGCTGTAAAACTCTTGCAGAAACGTATTGAAGTTAGAGTTGTCCGGCGGAAATTCAAGC ATACATTTGGAGAGGACCTCGTGGTGTGTCAGATTGGCATGTCCTCCTATGAATTTCCTCCAGCTCTGAA GCCACCAGAAGACCTGGTGGTGCTGGGTAAAGATGCTTCCGGGCAGCCAATTTTTAATGCTTCTGCCAAA CACTGGACCAATTTTGTCATTACAGAAAATGCAAATGATGCAATTGGTATCCTTAACAATTCTGCCTCAT TCAACAAGATGTCAATTGAATACAAATATGAGATGATGCCAAATCGCACATGGCGTTGTCGAGTGTTTTT ACAAGATCACTGCTTAGCTGAAGGTTATGGAACCAAGAAAACAAGTAAACATGCAGCTGCCGACGAGGCT TTGAAAATTCTTCAAAAAACACAGCCCACTTATCCATCTGTCAAAAGTTCACAATGCCATACAGGCTCTT CACCCAGAGGATCTGGAAAGAAGAAAGATATAAAGGATCTTGTAGTTTATGAGAATTCTTCAAATCCCGT GTGCACGCTGAACGACACAGCTCAGTTTAACCGAATGACAGTTGAGTATGTCTATGAAAGGATGACAGGC CTCCGCTGGAAATGCAAAGTGATTCTAGAGAGTGAAGTAATTGCAGAAGCAGTTGGGGTGAAGAAAACTG TCAAATATGAAGCTGCTGGGGAAGCTGTGAAAACCCTCAAAAAGACCCAGCCAACTGTCATTAACAACTT GAAGAAAGGAGCTGTTGAAGATGTGATTTCAAGAAATGAAATTCAGGGCCGCTCAGCAGAGGAGGCTTAC AAACAGCAAATCAAAGAAGATAATATTGGAAATCAGCTGCTGAGAAAGATGGGTTGGACTGGTGGTGGTT TAGGTAAATCTGGTGAGGGCATACGGGAGCCTATCTCAGTGAAAGAGCAGCATAAGCGGGAAGGGCTTGG TCTGGATGTAGAGAGGGTGAATAAAATTGCCAAGAGAGATATTGAACAGATCATCAGAAACTACGCCCGC TCCGAGAGCCACACAGATTTGACTTTCTCTAGAGAGCTGACTAATGATGAACGGAAGCAAATACATCAGA TTGCCCAGAAGTATGGTCTTAAGAGTAAGTCTCATGGGGTGGGCCATGATAGGTACCTAGTGGTAGGTAG AAAAAGACGGAAGGAAGACCTACTAGATCAGCTCAAACAGGAAGGCCAAGTGGGCCATTACGAGCTTGTT ATGCCTCAAGCAAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence >RC230446 representing NM_001173487 Red=Cloning site Green=Tags(s) MGFMLPLIFRYSPRLMEKILQMAEGIDIGEMPSYDLVLSKPSKGQKRHLSTCDGQNPPKKQAGSKFHARP RFEPVHFVASSSKDERQEDPYGPQTKEVNEQTHFASMPRDIYQDYTQDSFSIQDGNSQYCDSSGFILTKD QPVTANMYFDSGNPAPSTTSQQANSQSTPEPSPSQTFPESVVAEKQYFIEKLTATIWKNLSNPEMTSGSD KINYTYMLTRCIQACKTNPEYIYAPLKEIPPADIPKNKKLLTDGYACEVRCQNIYLTTGYAGSKNGSRDR ATELAVKLLQKRIEVRVVRRKFKHTFGEDLVVCQIGMSSYEFPPALKPPEDLVVLGKDASGQPIFNASAK HWTNFVITENANDAIGILNNSASFNKMSIEYKYEMMPNRTWRCRVFLQDHCLAEGYGTKKTSKHAAADEA LKILQKTQPTYPSVKSSQCHTGSSPRGSGKKKDIKDLVVYENSSNPVCTLNDTAQFNRMTVEYVYERMTG LRWKCKVILESEVIAEAVGVKKTVKYEAAGEAVKTLKKTQPTVINNLKKGAVEDVISRNEIQGRSAEEAY KQQIKEDNIGNQLLRKMGWTGGGLGKSGEGIREPISVKEQHKREGLGLDVERVNKIAKRDIEQIIRNYAR SESHTDLTFSRELTNDERKQIHQIAQKYGLKSKSHGVGHDRYLVVGRKRRKEDLLDQLKQEGQVGHYELV MPQAN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_001173487
ORF Size	2115 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping	Ambient
Reference Data
RefSeq	NM_001173487.1, NP_001166958.1
RefSeq ORF	2118 bp
Locus ID	55922
UniProt ID	O15226
Cytogenetics	Xq24
Protein Families	Transcription Factors
MW	79.9 kDa
Summary	This gene encodes a transcriptional repressor that interacts with specific negative regulatory elements to mediate transcriptional repression of certain nuclear factor kappa B responsive genes. The protein localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

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Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System TrueClones: Full length cDNA clones (Human/Mouse/Rat), expressing native proteins pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
SDS for RC230446

Resources

cDNA Clone Resources
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones Protocol: Lentivirus packaging Product Sheet: AAV AAV Biosafety Lentivirus and AAV FAQ Virus-Mediated Gene Delivery

"NM_001173487" in other vectors (4)

SKU	Description	Size	Price
RC230446L3	Lenti-ORF clone of NKRF (Myc-DDK-tagged)-Human NFKB repressing factor (NKRF), transcript variant 1	10 ug	$1,010.00
RC230446L4	Lenti-ORF clone of NKRF (mGFP-tagged)-Human NFKB repressing factor (NKRF), transcript variant 1	10 ug	$1,010.00
RG230446	NKRF (tGFP-tagged) - Human NFKB repressing factor (NKRF), transcript variant 1	10 ug	$910.00
SC329084	NKRF (untagged)-Human NFKB repressing factor (NKRF) transcript variant 1	10 ug	$711.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.