Repulsive Guidance Molecule A (RGMA) (NM_001166288) Human Tagged ORF Clone

CAT#: RC230080

RGMA (Myc-DDK-tagged)-Human RGM domain family, member A (RGMA), transcript variant 5

Product Images

Circular map for RC230080

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Repulsive Guidance Molecule A
• Synonyms: RGM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230080 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  RCATGGGTATGGGGAGAGGGGCAGGACGTTCAGCCCTGGGATTCTGGCCGACCCTCGCCTTCCTTCTCTG
  CAGCTTCCCCGCAGCCACCTCCCCGTGCAAGATCCTCAAGTGCAACTCTGAGTTCTGGAGCGCCACGTCG
  GGCAGCCACGCCCCAGCCTCAGACGACACCCCCGAGTTCTGTGCAGCCTTGCGCAGCTACGCCCTGTGCA
  CGCGGCGGACGGCCCGCACCTGCCGGGGTGACCTGGCCTACCACTCGGCCGTCCATGGCATAGAGGACCT
  CATGAGCCAGCACAACTGCTCCAAGGATGGCCCCACCTCGCAGCCACGCCTGCGCACGCTCCCACCGGCC
  GGAGACAGCCAGGAGCGCTCGGACAGCCCCGAGATCTGCCATTACGAGAAGAGCTTTCACAAGCACTCGG
  CCACCCCCAACTACACGCACTGTGGCCTCTTCGGGGACCCACACCTCAGGACTTTCACCGACCGCTTCCA
  GACCTGCAAGGTGCAGGGCGCCTGGCCGCTCATCGACAATAATTACCTGAACGTGCAGGTCACCAACACG
  CCTGTGCTGCCCGGCTCAGCGGCCACTGCCACCAGCAAGCTCACCATCATCTTCAAGAACTTCCAGGAGT
  GTGTGGACCAGAAGGTGTACCAGGCTGAGATGGACGAGCTCCCGGCCGCCTTCGTGGATGGCTCTAAGAA
  CGGTGGGGACAAGCACGGGGCCAACAGCCTGAAGATCACTGAGAAGGTGTCAGGCCAGCACGTGGAGATC
  CAGGCCAAGTACATCGGCACCACCATCGTGGTGCGCCAGGTGGGCCGCTACCTGACCTTTGCCGTCCGCA
  TGCCAGAGGAAGTGGTCAATGCTGTGGAGGACTGGGACAGCCAGGGTCTCTACCTCTGCCTGCGGGGCTG
  CCCCCTCAACCAGCAGATCGACTTCCAGGCCTTCCACACCAATGCTGAGGGCACCGGTGCCCGCAGGCTG
  GCAGCCGCCAGCCCTGCACCCACAGCCCCCGAGACCTTCCCATACGAGACAGCCGTGGCCAAGTGCAAGG
  AGAAGCTGCCGGTGGAGGACCTGTACTACCAGGCCTGCGTCTTCGACCTCCTCACCACGGGCGACGTGAA
  CTTCACACTGGCCGCCTACTACGCGTTGGAGGATGTCAAGATGCTCCACTCCAACAAAGACAAACTGCAC
  CTGTATGAGAGGACTCGGGACCTGCCAGGCAGGGCGGCTGCGGGGCTGCCCCTGGCCCCCCGGCCCCTCC
  TGGGCGCCCTGGTCCCGCTCCTGGCCCTGCTCCCTGTGTTCTGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230080 protein sequence
  Red=Cloning site Green=Tags(s)
  
  XWVWGEGQDVQPWDSGRPSPSFSAASPQPPPRARSSSATLSSGAPRRAATPQPQTTPPSSVQPCAATPCA
  RGGRPAPAGVTWPTTRPSMA*RTS*ASTTAPRMAPPRSHACARSHRPETARSARTAPRSAITRRAFTSTR
  PPPTTRTVASSGTHTSGLSPTASRPARCRAPGRSSTIIT*TCRSPTRLCCPAQRPLPPASSPSSSRTSRS
  VWTRRCTRLRWTSSRPPSWMALRTVGTSTGPTA*RSLRRCQASTWRSRPSTSAPPSWCARWAAT*PLPSA
  CQRKWSMLWRTGTARVSTSACGAAPSTSRSTSRPSTPMLRAPVPAGWQPPALHPQPPRPSHTRQPWPSAR
  RSCRWRTCTTRPASSTSSPRAT*TSHWPPTTRWRMSRCSTPTKTNCTCMRGLGTCQAGRLRGCPWPPGPS
  WAPWSRSWPCSLCS
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166288
• ORF Size: 1302 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166288.1, NP_001159760.1
• RefSeq Size: 3058 bp
• RefSeq ORF: 1305 bp
• Locus ID: 56963
• UniProt ID: Q96B86
• Cytogenetics: 15q26.1
• MW: 47.5 kDa
• Gene Summary: This gene encodes a member of the repulsive guidance molecule family. The encoded protein is a glycosylphosphatidylinositol-anchored glycoprotein that functions as an axon guidance protein in the developing and adult central nervous system. This protein may also function as a tumor suppressor in some cancers. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]