FAM120C (NM_198456) Human Tagged ORF Clone

CAT#: RC229744

FAM120C (Myc-DDK-tagged)-Human family with sequence similarity 120C (FAM120C), transcript variant 2

Product Images

Circular map for RC229744

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAM120C
• Synonyms: CXorf17; ORF34
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229744 representing NM_198456
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGTCCAGGGCTTCCAAGAGTTCCTGGAGAAGCGCTGTCCCGGGGCCGTGGTGCCCGTGGACCTCC
  TAAAACTCGCGCGCACGGTCTCGCGCCAGCAGCAGCAGCAGCACTTGCACCGCCAGCTGCCGCCGACTGC
  AGCCCTAGCGCCCGGGGCTCCACGCGCCGCCAGGGGCTCCGTGCCTCTGCAACCGCCGCTTCCGCCCGCT
  GCCTTGGGTGCCTACTCCGGGGGCGCGGGGCCGATTCGGCACCATCACCCCGCTCACCACTTCCACCATC
  ATGGCCAAGCGCAGCCCGGGCTGCACCCTCCGCTGCCGCCGCCGCCGCCCCCTCAGCTGCCCGGGGCCCG
  GGTGCTGGTGGACGCCGGCTCGGCGCTGCCGCGGCTCTATGGCGGCTACCAGACGGATTGGGTGTGTGGC
  GGCCAATGGAACGCCATGCTGGGCTACTTGTCAGCGCTGTGCCAGGCTTGTGCCTATCCTGGCGGCGACG
  GCCTGGAGCTCGTGGTCATGTTCCCGGGGGGCCTGGGCAAGGACCGGCTGGCCGAGTGGGGCCGTCGGTG
  CCAGGCCGAGCGGCAGACAGCGCAACTGATCGTGGGACACGTGGGCAACAAGGGCACCCCTCCACCGCGG
  GCCTGGTTCCTGCCACCGGCCTGCCTGAGCCACTGCGTGAGGCTAGCACTCATCCGCTTCCGGGTCAAGC
  AAGCTGCCATGTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229744 representing NM_198456
  Red=Cloning site Green=Tags(s)
  
  MGVQGFQEFLEKRCPGAVVPVDLLKLARTVSRQQQQQHLHRQLPPTAALAPGAPRAARGSVPLQPPLPPA
  ALGAYSGGAGPIRHHHPAHHFHHHGQAQPGLHPPLPPPPPPQLPGARVLVDAGSALPRLYGGYQTDWVCG
  GQWNAMLGYLSALCQACAYPGGDGLELVVMFPGGLGKDRLAEWGRRCQAERQTAQLIVGHVGNKGTPPPR
  AWFLPPACLSHCVRLALIRFRVKQAAML
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198456
• ORF Size: 714 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198456.1, NP_940858.1
• RefSeq ORF: 717 bp
• Locus ID: 54954
• UniProt ID: Q9NX05
• Cytogenetics: Xp11.22
• MW: 25.9 kDa
• Gene Summary: This gene encodes a potential transmembrane protein and lies in a region where mutations and deletions have been associated with intellectual disability and autism. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]