CRY2 (NM_021117) Human Tagged ORF Clone

CAT#: RC229309

CRY2 (Myc-DDK-tagged)-Human cryptochrome 2 (photolyase-like) (CRY2), transcript variant 1

Product Images

Circular map for RC229309

• 

  Western blot validation of overexpression lysate (Cat# LY432326) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229309 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CRY2
• Synonyms: HCRY2; PHLL2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229309 representing NM_021117
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGGGGTCCACGTCGCCTACCGGGGCGGAGCGGGGGTGGCTGGAGCAGTCTGGACAGTCATGGCGG
  CGACTGTGGCGACGGCGGCAGCTGTGGCCCCGGCGCCAGCGCCCGGCACGGACAGCGCCTCTTCGGTGCA
  CTGGTTCCGCAAAGGGCTGCGACTCCACGACAACCCGGCGTTGCTGGCGGCCGTGCGCGGGGCGCGCTGC
  GTGCGCTGCGTTTACATTCTCGACCCGTGGTTCGCGGCCTCCTCCTCAGTCGGGATCAACCGATGGAGGT
  TCCTACTTCAGTCTCTGGAAGATTTGGACACAAGTTTAAGGAAACTGAACTCCCGCCTGTTTGTAGTCCG
  GGGACAGCCAGCCGACGTGTTCCCAAGGCTGTTCAAGGAATGGGGAGTGACCCGCTTGACCTTTGAATAT
  GACTCTGAACCCTTTGGGAAAGAACGGGATGCAGCCATCATGAAGATGGCCAAGGAGGCTGGTGTGGAAG
  TAGTGACGGAGAATTCTCATACCCTCTATGACCTGGACAGGATCATTGAGCTGAATGGGCAGAAGCCACC
  CCTTACATACAAGCGCTTTCAGGCCATCATCAGCCGCATGGAGCTGCCCAAGAAGCCAGTGGGCTTGGTG
  ACCAGCCAGCAGATGGAGAGCTGCAGGGCCGAGATCCAGGAGAACCACGACGAGACCTACGGCGTGCCCT
  CCCTGGAGGAGCTGGGGTTCCCCACTGAAGGACTTGGTCCAGCTGTCTGGCAGGGAGGAGAGACAGAAGC
  TCTGGCCCGCCTGGATAAGCACTTGGAACGGAAGGCCTGGGTTGCCAACTATGAGAGACCCCGAATGAAC
  GCCAACTCCCTCCTGGCCAGCCCCACAGGCCTCAGCCCCTACCTGCGCTTTGGTTGTCTCTCCTGCCGCC
  TCTTCTACTACCGCCTGTGGGACCTGTATAAAAAGGTGAAGCGGAACAGCACACCTCCCCTCTCCCTATT
  TGGGCAACTCCTATGGCGAGAGTTCTTCTACACGGCAGCTACCAACAACCCCAGGTTTGACCGCATGGAG
  GGGAACCCCATCTGCATCCAGATCCCCTGGGACCGCAATCCTGAGGCCCTGGCCAAGTGGGCTGAGGGCA
  AGACAGGCTTCCCTTGGATTGATGCCATCATGACCCAACTGAGGCAGGAGGGCTGGATCCACCACCTGGC
  CCGGCATGCCGTGGCCTGCTTCCTGACCCGCGGGGACCTCTGGGTCAGCTGGGAGAGCGGGGTCCGGGTA
  TTTGATGAGCTGCTCCTGGATGCAGATTTCAGCGTGAACGCAGGCAGCTGGATGTGGCTGTCCTGCAGTG
  CTTTCTTCCAGCAGTTCTTCCACTGCTACTGCCCTGTGGGCTTTGGCCGTCGCACGGACCCCAGTGGGGA
  CTACATCAGGCGATACCTGCCCAAATTGAAAGCGTTCCCCTCTCGATACATCTATGAGCCCTGGAATGCC
  CCAGAGTCAATTCAGAAGGCAGCCAAGTGCATCATTGGTGTGGACTACCCACGGCCCATCGTCAACCATG
  CCGAGACCAGCCGGCTTAACATTGAACGAATGAAGCAGATTTACCAGCAGCTTTCGCGCTACCGGGGACT
  CTGTCTACTGGCATCTGTCCCTTCCTGTGTGGAAGACCTCAGTCACCCTGTGGCAGAGCCCAGCTCGAGC
  CAGGCTGGCAGCATGAGCAGTGCAGGCCCAAGACCACTACCCAGTGGCCCAGCATCCCCCAAACGCAAGC
  TGGAAGCAGCCGAGGAACCACCTGGTGAAGAACTCAGCAAACGGGCCCGGGTGGCAGAGTTGCCAACCCC
  AGAGCTGCCGAGCAAGGATGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229309 representing NM_021117
  Red=Cloning site Green=Tags(s)
  
  MGGVHVAYRGGAGVAGAVWTVMAATVATAAAVAPAPAPGTDSASSVHWFRKGLRLHDNPALLAAVRGARC
  VRCVYILDPWFAASSSVGINRWRFLLQSLEDLDTSLRKLNSRLFVVRGQPADVFPRLFKEWGVTRLTFEY
  DSEPFGKERDAAIMKMAKEAGVEVVTENSHTLYDLDRIIELNGQKPPLTYKRFQAIISRMELPKKPVGLV
  TSQQMESCRAEIQENHDETYGVPSLEELGFPTEGLGPAVWQGGETEALARLDKHLERKAWVANYERPRMN
  ANSLLASPTGLSPYLRFGCLSCRLFYYRLWDLYKKVKRNSTPPLSLFGQLLWREFFYTAATNNPRFDRME
  GNPICIQIPWDRNPEALAKWAEGKTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWVSWESGVRV
  FDELLLDADFSVNAGSWMWLSCSAFFQQFFHCYCPVGFGRRTDPSGDYIRRYLPKLKAFPSRYIYEPWNA
  PESIQKAAKCIIGVDYPRPIVNHAETSRLNIERMKQIYQQLSRYRGLCLLASVPSCVEDLSHPVAEPSSS
  QAGSMSSAGPRPLPSGPASPKRKLEAAEEPPGEELSKRARVAELPTPELPSKDA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021117
• ORF Size: 1842 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021117.3, NP_066940.2
• RefSeq ORF: 1782 bp
• Locus ID: 1408
• UniProt ID: Q49AN0
• Cytogenetics: 11p11.2
• Protein Families: Druggable Genome
• Protein Pathways: Circadian rhythm - mammal
• MW: 68.8 kDa
• Gene Summary: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]