PNCK (NM_001039582) Human Tagged ORF Clone

CAT#: RC229215

PNCK (Myc-DDK-tagged)-Human pregnancy up-regulated non-ubiquitously expressed CaM kinase (PNCK), transcript variant 1

Product images

Western blot validation of overexpression lysate (Cat# LY432237) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229215 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PNCK
• Synonyms: BSTK3; CaMK1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229215 representing NM_001039582
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCTGCTTTCGGGCAAGTGGCCGGGTCAGCCTGTCCTCGGAGAGGCGGTGAAGGCAGAGACTGGA
  AGGCAGAGAGCCTTGCCGACCTGTGGCCGAAGAGCTCTCCGGGAGACAGCCACCGGTGGTGCAAAGGCCC
  TGGGGCCGGCCCAGCCGGGCCGCAGCTCCGGGAGGCGGCGCGAGCGAGCAGTGGGCTGGGCGGTGGCGGC
  CGGCACCCGAGCCGGATCCCGGCGATTGCCTTACAAGACATGCTGCTGCTGAAGAAACACACGGAGGACA
  TCAGCAGCGTCTACGAGATCCGCGAGAGGCTCGGCTCGGGTGCCTTCTCCGAGGTGGTGCTGGCCCAGGA
  GCGGGGCTCCGCACACCTCGTGGCCCTCAAGTGCATCCCCAAGAAGGCCCTCCGGGGCAAGGAGGCCCTG
  GTGGAGAACGAGATCGCAGTGCTCCGTAGGATCAGTCACCCCAACATCGTCGCTCTGGAGGATGTCCACG
  AGAGCCCTTCCCACCTCTACCTGGCCATGGAACTGGTGACGGGTGGCGAGCTGTTTGACCGCATCATGGA
  GCGCGGCTCCTACACAGAGAAGGATGCCAGCCATCTGGTGGGTCAGGTCCTTGGCGCCGTCTCCTACCTG
  CACAGCCTGGGGATCGTGCACCGGGACCTCAAGCCCGAAAACCTCCTGTATGCCACGCCCTTTGAGGACT
  CGAAGATCATGGTCTCTGACTTTGGACTCTCCAAAATCCAGGCTGGGAACATGCTAGGCACCGCCTGTGG
  GACCCCTGGATATGTGGCCCCAGAGCTCTTGGAGCAGAAACCCTACGGGAAGGCCGTAGATGTGTGGGCC
  CTGGGCGTCATCTCCTACATCCTGCTGTGTGGGTACCCCCCCTTCTACGACGAGAGCGACCCTGAGCTCT
  TCAGCCAGATCCTGAGGGCCAGCTATGAGTTTGACTCTCCTTTCTGGGATGACATCTCAGAATCAGCCAA
  AGACTTCATCCGGCACCTTCTGGAGCGAGACCCCCAGAAGAGGTTCACCTGCCAACAGGCCTTGCGGCAC
  CTTTGGATCTCTGGGGACACAGCCTTCGACAGGGACATCTTAGGCTCTGTCAGTGAGCAGATCCGGAAGA
  ACTTTGCTCGGACACACTGGAAGCGAGCCTTCAATGCCACCTCGTTCCTGCGCCACATCCGGAAGCTGGG
  GCAGATCCCAGAGGGCGAGGGGGCCTCTGAGCAGGGCATGGCCCGCCACAGCCACTCAGGCCTCCGTGCT
  GGCCAGCCCCCCAAGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229215 representing NM_001039582
  Red=Cloning site Green=Tags(s)
  
  MEAAFGQVAGSACPRRGGEGRDWKAESLADLWPKSSPGDSHRWCKGPGAGPAGPQLREAARASSGLGGGG
  RHPSRIPAIALQDMLLLKKHTEDISSVYEIRERLGSGAFSEVVLAQERGSAHLVALKCIPKKALRGKEAL
  VENEIAVLRRISHPNIVALEDVHESPSHLYLAMELVTGGELFDRIMERGSYTEKDASHLVGQVLGAVSYL
  HSLGIVHRDLKPENLLYATPFEDSKIMVSDFGLSKIQAGNMLGTACGTPGYVAPELLEQKPYGKAVDVWA
  LGVISYILLCGYPPFYDESDPELFSQILRASYEFDSPFWDDISESAKDFIRHLLERDPQKRFTCQQALRH
  LWISGDTAFDRDILGSVSEQIRKNFARTHWKRAFNATSFLRHIRKLGQIPEGEGASEQGMARHSHSGLRA
  GQPPKW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039582
• ORF Size: 1278 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039582.3, NP_001034671.3
• RefSeq ORF: 1281 bp
• Locus ID: 139728
• UniProt ID: Q6P2M8
• Cytogenetics: Xq28
• Protein Families: Druggable Genome, Protein Kinase
• MW: 46.8 kDa
• Gene Summary: PNCK is a member of the calcium/calmodulin-dependent protein kinase family of protein serine/threonine kinases (see CAMK1; MIM 604998) (Gardner et al., 2000 [PubMed 10673339]).[supplied by OMIM, Mar 2008]