PNCK (NM_001039582) Human Tagged ORF Clone

CAT#: RC229215

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PNCK (Myc-DDK-tagged)-Human pregnancy up-regulated non-ubiquitously expressed CaM kinase (PNCK), transcript variant 1



  "NM_001039582" in other vectors (5)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PNCK
Synonyms BSTK3; CaMK1b
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC229215 representing NM_001039582
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCTGCTTTCGGGCAAGTGGCCGGGTCAGCCTGTCCTCGGAGAGGCGGTGAAGGCAGAGACTGGA
AGGCAGAGAGCCTTGCCGACCTGTGGCCGAAGAGCTCTCCGGGAGACAGCCACCGGTGGTGCAAAGGCCC
TGGGGCCGGCCCAGCCGGGCCGCAGCTCCGGGAGGCGGCGCGAGCGAGCAGTGGGCTGGGCGGTGGCGGC
CGGCACCCGAGCCGGATCCCGGCGATTGCCTTACAAGACATGCTGCTGCTGAAGAAACACACGGAGGACA
TCAGCAGCGTCTACGAGATCCGCGAGAGGCTCGGCTCGGGTGCCTTCTCCGAGGTGGTGCTGGCCCAGGA
GCGGGGCTCCGCACACCTCGTGGCCCTCAAGTGCATCCCCAAGAAGGCCCTCCGGGGCAAGGAGGCCCTG
GTGGAGAACGAGATCGCAGTGCTCCGTAGGATCAGTCACCCCAACATCGTCGCTCTGGAGGATGTCCACG
AGAGCCCTTCCCACCTCTACCTGGCCATGGAACTGGTGACGGGTGGCGAGCTGTTTGACCGCATCATGGA
GCGCGGCTCCTACACAGAGAAGGATGCCAGCCATCTGGTGGGTCAGGTCCTTGGCGCCGTCTCCTACCTG
CACAGCCTGGGGATCGTGCACCGGGACCTCAAGCCCGAAAACCTCCTGTATGCCACGCCCTTTGAGGACT
CGAAGATCATGGTCTCTGACTTTGGACTCTCCAAAATCCAGGCTGGGAACATGCTAGGCACCGCCTGTGG
GACCCCTGGATATGTGGCCCCAGAGCTCTTGGAGCAGAAACCCTACGGGAAGGCCGTAGATGTGTGGGCC
CTGGGCGTCATCTCCTACATCCTGCTGTGTGGGTACCCCCCCTTCTACGACGAGAGCGACCCTGAGCTCT
TCAGCCAGATCCTGAGGGCCAGCTATGAGTTTGACTCTCCTTTCTGGGATGACATCTCAGAATCAGCCAA
AGACTTCATCCGGCACCTTCTGGAGCGAGACCCCCAGAAGAGGTTCACCTGCCAACAGGCCTTGCGGCAC
CTTTGGATCTCTGGGGACACAGCCTTCGACAGGGACATCTTAGGCTCTGTCAGTGAGCAGATCCGGAAGA
ACTTTGCTCGGACACACTGGAAGCGAGCCTTCAATGCCACCTCGTTCCTGCGCCACATCCGGAAGCTGGG
GCAGATCCCAGAGGGCGAGGGGGCCTCTGAGCAGGGCATGGCCCGCCACAGCCACTCAGGCCTCCGTGCT
GGCCAGCCCCCCAAGTGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC229215 representing NM_001039582
Red=Cloning site Green=Tags(s)

MEAAFGQVAGSACPRRGGEGRDWKAESLADLWPKSSPGDSHRWCKGPGAGPAGPQLREAARASSGLGGGG
RHPSRIPAIALQDMLLLKKHTEDISSVYEIRERLGSGAFSEVVLAQERGSAHLVALKCIPKKALRGKEAL
VENEIAVLRRISHPNIVALEDVHESPSHLYLAMELVTGGELFDRIMERGSYTEKDASHLVGQVLGAVSYL
HSLGIVHRDLKPENLLYATPFEDSKIMVSDFGLSKIQAGNMLGTACGTPGYVAPELLEQKPYGKAVDVWA
LGVISYILLCGYPPFYDESDPELFSQILRASYEFDSPFWDDISESAKDFIRHLLERDPQKRFTCQQALRH
LWISGDTAFDRDILGSVSEQIRKNFARTHWKRAFNATSFLRHIRKLGQIPEGEGASEQGMARHSHSGLRA
GQPPKW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001039582
ORF Size 1278 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001039582.3, NP_001034671.3
RefSeq ORF 1281 bp
Locus ID 139728
UniProt ID Q6P2M8
Cytogenetics Xq28
Protein Families Druggable Genome, Protein Kinase
MW 46.8 kDa
Gene Summary PNCK is a member of the calcium/calmodulin-dependent protein kinase family of protein serine/threonine kinases (see CAMK1; MIM 604998) (Gardner et al., 2000 [PubMed 10673339]).[supplied by OMIM, Mar 2008]

Other Versions

Other products for "PNCK"
Frequently bought together (5)
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pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 55.00

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