Myocardin (MYOCD) (NM_001146312) Human Tagged ORF Clone

CAT#: RC229055

MYOCD (Myc-DDK-tagged)-Human myocardin (MYOCD), transcript variant 1

Product Images

Circular map for RC229055

• 

  Western blot validation of overexpression lysate (Cat# LY432082) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229055 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Myocardin
• Synonyms: MGBL; MYCD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC229055 representing NM_001146312
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACACTCCTGGGGTCTGAGCATTCCTTGCTGATTAGGAGCAAGTTCAGATCAGTTTTACAGTTAAGAC
  TTCAACAAAGAAGGACCCAGGAACAACTGGCTAACCAAGGCATAATACCACCACTGAAACGTCCAGCTGA
  ATTCCATGAGCAAAGAAAACATTTGGATAGTGACAAGGCTAAAAATTCCCTGAAGCGCAAAGCCAGAAAC
  AGGTGCAACAGTGCCGACTTGGTTAATATGCACATACTCCAAGCTTCCACTGCAGAGAGGTCCATTCCAA
  CTGCTCAGATGAAGCTGAAAAGAGCCCGACTCGCCGATGATCTCAATGAAAAAATTGCTCTACGACCAGG
  GCCACTGGAGCTGGTGGAAAAAAACATTCTTCCTGTGGATTCTGCTGTGAAAGAGGCCATAAAAGGTAAC
  CAGGTGAGTTTCTCCAAATCCACGGATGCTTTTGCCTTTGAAGAGGACAGCAGCAGCGATGGGCTTTCTC
  CGGATCAGACTCGAAGTGAAGACCCCCAAAACTCAGCGGGATCCCCGCCAGACGCTAAAGCCTCAGATAC
  CCCTTCGACAGGTTCTCTGGGGACAAACCAGGATCTTGCTTCTGGCTCAGAAAATGACAGAAATGACTCA
  GCCTCACAGCCCAGCCACCAGTCAGATGCGGGGAAGCAGGGGCTTGGCCCCCCCAGCACCCCCATAGCCG
  TGCATGCTGCTGTAAAGTCCAAATCCTTGGGTGACAGTAAGAACCGCCACAAAAAGCCCAAGGACCCCAA
  GCCAAAGGTGAAGAAGCTTAAATATCACCAGTACATTCCCCCAGACCAGAAGGCAGAGAAGTCCCCTCCA
  CCTATGGACTCAGCCTACGCTCGGCTGCTCCAGCAACAGCAGCTGTTCCTGCAGCTCCAAATCCTCAGCC
  AGCAGCAGCAGCAGCAGCAACACCGGTTCAGCTACCTAGGGATGCACCAAGCTCAGCTTAAGGAACCAAA
  TGAACAGATGGTCAGAAATCCAAACTCTTCTTCAACGCCACTGAGCAATACCCCCTTGTCTCCTGTCAAA
  AACAGTTTTTCTGGACAAACTGGTGTCTCTTCTTTCAAACCAGGCCCACTCCCACCTAACCTGGATGATC
  TGAAGGTCTCTGAATTAAGACAACAGCTTCGAATTCGGGGCTTGCCTGTGTCAGGCACCAAAACGGCTCT
  CATGGACCGGCTTCGACCCTTCCAGGACTGCTCTGGCAACCCAGTGCCGAACTTTGGGGATATAACGACT
  GTCACTTTTCCTGTCACACCCAACACGCTGCCCAATTACCAGTCTTCCTCTTCTACCAGTGCCCTGTCCA
  ACGGCTTCTACCACTTTGGCAGCACCAGCTCCAGCCCCCCGATCTCCCCAGCCTCCTCTGACCTGTCAGT
  CGCTGGGTCCCTGCCGGACACCTTCAATGATGCCTCCCCCTCCTTCGGCCTGCACCCGTCCCCAGTCCAC
  GTGTGCACGGAGGAAAGTCTCATGAGCAGCCTGAATGGGGGCTCTGTTCCTTCTGAGCTGGATGGGCTGG
  ACTCCGAGAAGGACAAGATGCTGGTGGAGAAGCAGAAGGTGATCAATGAACTCACCTGGAAACTCCAGCA
  AGAGCAGAGGCAGGTGGAGGAGCTGAGGATGCAGCTTCAGAAGCAGAAAAGGAATAACTGTTCAGAGAAG
  AAGCCGCTGCCTTTCCTGGCTGCCTCCATCAAGCAGGAAGAGGCTGTCTCCAGCTGTCCTTTTGCATCCC
  AAGTACCTGTGAAAAGACAAAGCAGCAGCTCAGAGTGTCACCCACCGGCTTGTGAAGCTGCTCAACTCCA
  GCCTCTTGGAAATGCTCATTGTGTGGAGTCCTCAGATCAAACCAATGTACTTTCTTCCACATTTCTCAGC
  CCCCAGTGTTCCCCTCAGCATTCACCGCTGGGGGCTGTGAAAAGCCCACAGCACATCAGTTTGCCCCCAT
  CACCCAACAACCCTCACTTTCTGCCCTCATCCTCCGGGGCCCAGGGAGAAGGGCACAGGGTCTCCTCGCC
  CATCAGCAGCCAGGTGTGCACTGCACAGAACTCAGGAGCACACGATGGCCATCCTCCAAGCTTCTCTCCC
  CATTCTTCCAGCCTCCACCCGCCCTTCTCTGGAGCCCAAGCAGACAGCAGTCATGGTGCCGGGGGAAACC
  CTTGTCCCAAAAGCCCATGTGTACAGCAAAAGATGGCTGGTTTACACTCTTCTGATAAGGTGGGGCCAAA
  GTTTTCAATTCCATCCCCAACTTTTTCTAAGTCAAGTTCAGCAATTTCAGAGGTAACACAGCCTCCATCC
  TATGAAGATGCCGTAAAGCAGCAAATGACCCGGAGTCAGCAGATGGATGAACTCCTGGACGTGCTTATTG
  AAAGCGGAGAAATGCCAGCAGACGCTAGAGAGGATCACTCATGTCTTCAAAAAGTCCCAAAGATACCCAG
  ATCTTCCCGAAGTCCAACTGCTGTCCTCACCAAGCCCTCGGCTTCCTTTGAACAAGCCTCTTCAGGCAGC
  CAGATCCCCTTTGATCCCTATGCCACCGACAGTGATGAGCATCTTGAAGTCTTATTAAATTCCCAGAGCC
  CCCTAGGAAAGATGAGTGATGTCACCCTTCTAAAAATTGGGAGCGAAGAGCCTCACTTTGATGGGATAAT
  GGATGGATTCTCTGGGAAGGCTGCAGAAGACCTCTTCAATGCACATGAGATCTTGCCAGGCCCCCTCTCT
  CCAATGCAGACACAGTTTTCACCCTCTTCTGTGGACAGCAATGGGCTGCAGTTAAGCTTCACTGAATCTC
  CCTGGGAAACCATGGAGTGGCTGGACCTCACTCCGCCAAATTCCACACCAGGCTTTAGCGCCCTCACCAC
  CAGCAGCCCCAGCATCTTCAACATCGATTTCCTGGATGTCACTGATCTCAATTTGAATTCTTCCATGGAC
  CTTCACTTGCAGCAGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC229055 representing NM_001146312
  Red=Cloning site Green=Tags(s)
  
  MTLLGSEHSLLIRSKFRSVLQLRLQQRRTQEQLANQGIIPPLKRPAEFHEQRKHLDSDKAKNSLKRKARN
  RCNSADLVNMHILQASTAERSIPTAQMKLKRARLADDLNEKIALRPGPLELVEKNILPVDSAVKEAIKGN
  QVSFSKSTDAFAFEEDSSSDGLSPDQTRSEDPQNSAGSPPDAKASDTPSTGSLGTNQDLASGSENDRNDS
  ASQPSHQSDAGKQGLGPPSTPIAVHAAVKSKSLGDSKNRHKKPKDPKPKVKKLKYHQYIPPDQKAEKSPP
  PMDSAYARLLQQQQLFLQLQILSQQQQQQQHRFSYLGMHQAQLKEPNEQMVRNPNSSSTPLSNTPLSPVK
  NSFSGQTGVSSFKPGPLPPNLDDLKVSELRQQLRIRGLPVSGTKTALMDRLRPFQDCSGNPVPNFGDITT
  VTFPVTPNTLPNYQSSSSTSALSNGFYHFGSTSSSPPISPASSDLSVAGSLPDTFNDASPSFGLHPSPVH
  VCTEESLMSSLNGGSVPSELDGLDSEKDKMLVEKQKVINELTWKLQQEQRQVEELRMQLQKQKRNNCSEK
  KPLPFLAASIKQEEAVSSCPFASQVPVKRQSSSSECHPPACEAAQLQPLGNAHCVESSDQTNVLSSTFLS
  PQCSPQHSPLGAVKSPQHISLPPSPNNPHFLPSSSGAQGEGHRVSSPISSQVCTAQNSGAHDGHPPSFSP
  HSSSLHPPFSGAQADSSHGAGGNPCPKSPCVQQKMAGLHSSDKVGPKFSIPSPTFSKSSSAISEVTQPPS
  YEDAVKQQMTRSQQMDELLDVLIESGEMPADAREDHSCLQKVPKIPRSSRSPTAVLTKPSASFEQASSGS
  QIPFDPYATDSDEHLEVLLNSQSPLGKMSDVTLLKIGSEEPHFDGIMDGFSGKAAEDLFNAHEILPGPLS
  PMQTQFSPSSVDSNGLQLSFTESPWETMEWLDLTPPNSTPGFSALTTSSPSIFNIDFLDVTDLNLNSSMD
  LHLQQW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146312
• ORF Size: 2958 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_001146312.3
• RefSeq ORF: 2961 bp
• Locus ID: 93649
• UniProt ID: Q8IZQ8
• Cytogenetics: 17p12
• Protein Families: Transcription Factors
• MW: 106.6 kDa
• Gene Summary: This gene encodes a nuclear protein, which is expressed in heart, aorta, and in smooth muscle cell-containing tissues. It functions as a transcriptional co-activator of serum response factor (SRF) and modulates expression of cardiac and smooth muscle-specific SRF-target genes, and thus may play a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]