Myocardin (MYOCD) (NM_001146312) Human Tagged ORF Clone

CAT#: RC229055

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MYOCD (Myc-DDK-tagged)-Human myocardin (MYOCD), transcript variant 1



Reconstitution Protocol

USD 1,354.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MYOCD
Synonyms MGBL; MYCD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC229055 representing NM_001146312
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACACTCCTGGGGTCTGAGCATTCCTTGCTGATTAGGAGCAAGTTCAGATCAGTTTTACAGTTAAGAC
TTCAACAAAGAAGGACCCAGGAACAACTGGCTAACCAAGGCATAATACCACCACTGAAACGTCCAGCTGA
ATTCCATGAGCAAAGAAAACATTTGGATAGTGACAAGGCTAAAAATTCCCTGAAGCGCAAAGCCAGAAAC
AGGTGCAACAGTGCCGACTTGGTTAATATGCACATACTCCAAGCTTCCACTGCAGAGAGGTCCATTCCAA
CTGCTCAGATGAAGCTGAAAAGAGCCCGACTCGCCGATGATCTCAATGAAAAAATTGCTCTACGACCAGG
GCCACTGGAGCTGGTGGAAAAAAACATTCTTCCTGTGGATTCTGCTGTGAAAGAGGCCATAAAAGGTAAC
CAGGTGAGTTTCTCCAAATCCACGGATGCTTTTGCCTTTGAAGAGGACAGCAGCAGCGATGGGCTTTCTC
CGGATCAGACTCGAAGTGAAGACCCCCAAAACTCAGCGGGATCCCCGCCAGACGCTAAAGCCTCAGATAC
CCCTTCGACAGGTTCTCTGGGGACAAACCAGGATCTTGCTTCTGGCTCAGAAAATGACAGAAATGACTCA
GCCTCACAGCCCAGCCACCAGTCAGATGCGGGGAAGCAGGGGCTTGGCCCCCCCAGCACCCCCATAGCCG
TGCATGCTGCTGTAAAGTCCAAATCCTTGGGTGACAGTAAGAACCGCCACAAAAAGCCCAAGGACCCCAA
GCCAAAGGTGAAGAAGCTTAAATATCACCAGTACATTCCCCCAGACCAGAAGGCAGAGAAGTCCCCTCCA
CCTATGGACTCAGCCTACGCTCGGCTGCTCCAGCAACAGCAGCTGTTCCTGCAGCTCCAAATCCTCAGCC
AGCAGCAGCAGCAGCAGCAACACCGGTTCAGCTACCTAGGGATGCACCAAGCTCAGCTTAAGGAACCAAA
TGAACAGATGGTCAGAAATCCAAACTCTTCTTCAACGCCACTGAGCAATACCCCCTTGTCTCCTGTCAAA
AACAGTTTTTCTGGACAAACTGGTGTCTCTTCTTTCAAACCAGGCCCACTCCCACCTAACCTGGATGATC
TGAAGGTCTCTGAATTAAGACAACAGCTTCGAATTCGGGGCTTGCCTGTGTCAGGCACCAAAACGGCTCT
CATGGACCGGCTTCGACCCTTCCAGGACTGCTCTGGCAACCCAGTGCCGAACTTTGGGGATATAACGACT
GTCACTTTTCCTGTCACACCCAACACGCTGCCCAATTACCAGTCTTCCTCTTCTACCAGTGCCCTGTCCA
ACGGCTTCTACCACTTTGGCAGCACCAGCTCCAGCCCCCCGATCTCCCCAGCCTCCTCTGACCTGTCAGT
CGCTGGGTCCCTGCCGGACACCTTCAATGATGCCTCCCCCTCCTTCGGCCTGCACCCGTCCCCAGTCCAC
GTGTGCACGGAGGAAAGTCTCATGAGCAGCCTGAATGGGGGCTCTGTTCCTTCTGAGCTGGATGGGCTGG
ACTCCGAGAAGGACAAGATGCTGGTGGAGAAGCAGAAGGTGATCAATGAACTCACCTGGAAACTCCAGCA
AGAGCAGAGGCAGGTGGAGGAGCTGAGGATGCAGCTTCAGAAGCAGAAAAGGAATAACTGTTCAGAGAAG
AAGCCGCTGCCTTTCCTGGCTGCCTCCATCAAGCAGGAAGAGGCTGTCTCCAGCTGTCCTTTTGCATCCC
AAGTACCTGTGAAAAGACAAAGCAGCAGCTCAGAGTGTCACCCACCGGCTTGTGAAGCTGCTCAACTCCA
GCCTCTTGGAAATGCTCATTGTGTGGAGTCCTCAGATCAAACCAATGTACTTTCTTCCACATTTCTCAGC
CCCCAGTGTTCCCCTCAGCATTCACCGCTGGGGGCTGTGAAAAGCCCACAGCACATCAGTTTGCCCCCAT
CACCCAACAACCCTCACTTTCTGCCCTCATCCTCCGGGGCCCAGGGAGAAGGGCACAGGGTCTCCTCGCC
CATCAGCAGCCAGGTGTGCACTGCACAGAACTCAGGAGCACACGATGGCCATCCTCCAAGCTTCTCTCCC
CATTCTTCCAGCCTCCACCCGCCCTTCTCTGGAGCCCAAGCAGACAGCAGTCATGGTGCCGGGGGAAACC
CTTGTCCCAAAAGCCCATGTGTACAGCAAAAGATGGCTGGTTTACACTCTTCTGATAAGGTGGGGCCAAA
GTTTTCAATTCCATCCCCAACTTTTTCTAAGTCAAGTTCAGCAATTTCAGAGGTAACACAGCCTCCATCC
TATGAAGATGCCGTAAAGCAGCAAATGACCCGGAGTCAGCAGATGGATGAACTCCTGGACGTGCTTATTG
AAAGCGGAGAAATGCCAGCAGACGCTAGAGAGGATCACTCATGTCTTCAAAAAGTCCCAAAGATACCCAG
ATCTTCCCGAAGTCCAACTGCTGTCCTCACCAAGCCCTCGGCTTCCTTTGAACAAGCCTCTTCAGGCAGC
CAGATCCCCTTTGATCCCTATGCCACCGACAGTGATGAGCATCTTGAAGTCTTATTAAATTCCCAGAGCC
CCCTAGGAAAGATGAGTGATGTCACCCTTCTAAAAATTGGGAGCGAAGAGCCTCACTTTGATGGGATAAT
GGATGGATTCTCTGGGAAGGCTGCAGAAGACCTCTTCAATGCACATGAGATCTTGCCAGGCCCCCTCTCT
CCAATGCAGACACAGTTTTCACCCTCTTCTGTGGACAGCAATGGGCTGCAGTTAAGCTTCACTGAATCTC
CCTGGGAAACCATGGAGTGGCTGGACCTCACTCCGCCAAATTCCACACCAGGCTTTAGCGCCCTCACCAC
CAGCAGCCCCAGCATCTTCAACATCGATTTCCTGGATGTCACTGATCTCAATTTGAATTCTTCCATGGAC
CTTCACTTGCAGCAGTGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC229055 representing NM_001146312
Red=Cloning site Green=Tags(s)

MTLLGSEHSLLIRSKFRSVLQLRLQQRRTQEQLANQGIIPPLKRPAEFHEQRKHLDSDKAKNSLKRKARN
RCNSADLVNMHILQASTAERSIPTAQMKLKRARLADDLNEKIALRPGPLELVEKNILPVDSAVKEAIKGN
QVSFSKSTDAFAFEEDSSSDGLSPDQTRSEDPQNSAGSPPDAKASDTPSTGSLGTNQDLASGSENDRNDS
ASQPSHQSDAGKQGLGPPSTPIAVHAAVKSKSLGDSKNRHKKPKDPKPKVKKLKYHQYIPPDQKAEKSPP
PMDSAYARLLQQQQLFLQLQILSQQQQQQQHRFSYLGMHQAQLKEPNEQMVRNPNSSSTPLSNTPLSPVK
NSFSGQTGVSSFKPGPLPPNLDDLKVSELRQQLRIRGLPVSGTKTALMDRLRPFQDCSGNPVPNFGDITT
VTFPVTPNTLPNYQSSSSTSALSNGFYHFGSTSSSPPISPASSDLSVAGSLPDTFNDASPSFGLHPSPVH
VCTEESLMSSLNGGSVPSELDGLDSEKDKMLVEKQKVINELTWKLQQEQRQVEELRMQLQKQKRNNCSEK
KPLPFLAASIKQEEAVSSCPFASQVPVKRQSSSSECHPPACEAAQLQPLGNAHCVESSDQTNVLSSTFLS
PQCSPQHSPLGAVKSPQHISLPPSPNNPHFLPSSSGAQGEGHRVSSPISSQVCTAQNSGAHDGHPPSFSP
HSSSLHPPFSGAQADSSHGAGGNPCPKSPCVQQKMAGLHSSDKVGPKFSIPSPTFSKSSSAISEVTQPPS
YEDAVKQQMTRSQQMDELLDVLIESGEMPADAREDHSCLQKVPKIPRSSRSPTAVLTKPSASFEQASSGS
QIPFDPYATDSDEHLEVLLNSQSPLGKMSDVTLLKIGSEEPHFDGIMDGFSGKAAEDLFNAHEILPGPLS
PMQTQFSPSSVDSNGLQLSFTESPWETMEWLDLTPPNSTPGFSALTTSSPSIFNIDFLDVTDLNLNSSMD
LHLQQW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001146312
ORF Size 2958 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001146312.3
RefSeq ORF 2961 bp
Locus ID 93649
UniProt ID Q8IZQ8
Cytogenetics 17p12
Protein Families Transcription Factors
MW 106.6 kDa
Gene Summary This gene encodes a nuclear protein, which is expressed in heart, aorta, and in smooth muscle cell-containing tissues. It functions as a transcriptional co-activator of serum response factor (SRF) and modulates expression of cardiac and smooth muscle-specific SRF-target genes, and thus may play a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
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