Synaptopodin (SYNPO) (NM_001166209) Human Tagged ORF Clone

CAT#: RC228617

SYNPO (Myc-DDK-tagged)-Human synaptopodin (SYNPO), transcript variant 4

Product Images

Circular map for RC228617

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Synaptopodin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228617 representing NM_001166209
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGTCCTCACCTCCCACCTCCGCCCCTTGCACCCAGCGAAGGGAGGCCTACCCCCTGCGCCTTCC
  AGATCCCTGATGGAAGCTACAGGTGTCTGGCTCTGGAAGCCGAGGAGAGCAGCGGTGAGGAGGGCCTGCA
  GGGAGAGGTGGGGCCTACCGACCTGGAAGAGGATGAGGGGGTCAGCAGGAGTGGGGACGACTCTGCCTGC
  AGAGTCACCCAGGGGACACCGCAGCTGCCCAAAGCCCTGGGCATCCAACCACCCAGCTGCTCCAGAGAGG
  AACAAGGGGCGTCCCAGCACGACGACAGGGCCAGCCAGGACTGGGATGTAGTGAAGGCCGGGCAGATGAT
  GACAGCCAGCCCCAGCCCTGGCCCTGGGCCCAGAGTGGCCCAGAAACCAGCGTTGGGCCGGAGCACTAGC
  CTCACGGAGAAGGATCTGAAAGAAGCCAAGGCGCGGAGCCAGCAGATTGCAGCCCAGCTGACCACCCCTC
  CCAGCTCCAATTCCCGTGGCGTCCAGCTCTTCAACAGGCGCCGGCAGAGGGTGAACGAGTTCACCTTGGA
  GAGCCACGGCCAGAGGGGACAGAAGCCCAGCCAGGAGTCCCTCAGAGTGCTCCCTTCAAGCCTCCCAGGC
  CATGCACCGGGGCTCAGCCTGAGTTCCACCTCGCTGCCGGAGCCAGGCCCTCCACGGCACCCCAGTCCCC
  AGAGCCCCGACAGAGGGGTCCCTGGCCACAGCATGGAGGGGTACTCAGAGGAGGCTAGCTTGCTGCGGCA
  CCTGGAGAAGGTGGCCAGTGAGGAGGAAGAGGTACCACTGGTGGTTTATCTAAAGGAGAATGCAGCACTG
  CTGACAGCCAATGGGCTGCACCTGTCCCAAAACCGAGAGGCCCAGCAGTCCTCACCGGCCCCACCTCCAG
  CTGAGGTCCACAGCCCAGCTGCAGATGTCAACCAAAACCTTGCCTCGCCCAGTGCCACGCTCACCACACC
  AACTTCTAACAGCAGCCACAATCCGCCAGCCACCGATGTCAATCAGAACCCACCGGCAACTGTTGTCCCA
  CAGAGCCTGCCACTTTCTAGCATCCAACAGAATTCCTCAGAGGCCCAACTCCCATCTAATGGCACAGGGC
  CTGCTTCCAAACCCAGCACCCTGTGTGCTGATGGGCAACCCCAGGCACCGGCTGAGGAGGTGAGATGCAG
  CACACTCCTAATTGACAAGGTATCAACTCCAGCTACCACCACCAGCACCTTCTCCAGAGAAGCTACGCTC
  ATCCCCAGCTCCAGGCCCCCAGCCTCAGATTTCATGTCCAGCTCCCTGCTCATTGACATCCAGCCCAACA
  CCCTAGTGGTGTCAGCAGATCAAGAGATGTCTGGGCGAGCAGCTGCCACCACGCCCACCAAGGTCTACAG
  TGAGGTCCACTTCACACTGGCCAAGCCCCCATCAGTGGTCAACAGGACGGCCAGGCCTTTTGGGATCCAG
  GCGCCAGGGGGCACCAGCCAGATGGAGAGGAGCCCCATGCTAGAGAGACGACATTTTGGGGAGAAGGCCC
  CGGCTCCCCAGCCCCCCAGTTTGCCAGACAGGAGCCCCCGGCCACAGAGACACATAATGTCCCGCAGCCC
  CATGGTGGAAAGGAGGATGATGGGGCAGCGAAGCCCGGCCTCAGAGAGACGCCCCTTGGGGAACTTCACT
  GCACCCCCCACCTACACTGAGACCTTGTCCACAGCCCCTCTGGCTTCCTGGGTGAGGTCTCCTCCCTCAT
  ATTCTGTCCTGTATCCCAGCTCCGACCCCAAGTCTTCTCATCTGAAGGGCCAGGCGGTTCCTGCCAGCAA
  GACGGGCATTCTGGAGGAGTCGATGGCCCGCCGGGGCAGCCGCAAATCCATGTTTACTTTCGTGGAGAAG
  CCCAAGGTGACCCCGAATCCAGACTTGCTGGATCTGGTACAGACAGCGGATGAGAAGCGGCGGCAGAGGG
  ACCAGGGGGAGGTAGGCGTGGAGGAGGAGCCCTTCGCACTGGGGGCCGAGGCCTCCAACTTCCAGCAGGA
  GCCAGCACCTCGTGACAGGGCCAGCCCCGCGGCGGCGGAGGAGGTGGTACCAGAGTGGGCCTCCTGCCTC
  AAGTCACCCCGCATCCAGGCCAAGCCGAAGCCCAAACCCAACCAGAACCTCTCCGAGGCCTCTGGGAAGG
  GAGCTGAGCTCTACGCCCGCCGCCAGTCACGGATGGAGAAATATGTCATCGAGTCTTCAAGCCACACGCC
  AGAGCTGGCCCGCTGCCCATCACCTACCATGTCCCTGCCTTCCTCCTGGAAATACCCCACTAACGCCCCC
  GGGGCCTTCCGAGTGGCATCCCGAAGCCCAGCCCGGACCCCGCCTGCCTCCCTCTACCATGGCTACCTGC
  CTGAGAACGGGGTCCTGCGCCCAGAGCCCACCAAGCAGCCGCCATACCAGCTGCGCCCCTCGCTCTTTGT
  CCTCTCACCTATCAAGGAGCCTGCCAAGGTCTCACCAAGAGCTGCCTCGCCCGCCAAGCCCAGCTCCTTG
  GACCTGGTGCCCAACCTGCCCAAGGGGGCTCTCCCTCCATCTCCTGCCCTGCCTCGGCCCTCGCGCTCCT
  CACCGGGCCTCTACACCTCCCCCGGCCAGGACAGCCTGCAGCCCACTGCCGTGAGCCCTCCTTACGGCGG
  TGACATCTCCCCCGTGTCTCCCTCCAGGGCGTGGTCTCCCCGAGCCAAGCAGGCCCCCAGGCCCTCCTTC
  TCTACCCGGAACGCCGGGATCGAGGCTCAGGTGTGGAAGCCTTCCTTCTGCTTCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228617 representing NM_001166209
  Red=Cloning site Green=Tags(s)
  
  MLGPHLPPPPLAPSEGRPTPCAFQIPDGSYRCLALEAEESSGEEGLQGEVGPTDLEEDEGVSRSGDDSAC
  RVTQGTPQLPKALGIQPPSCSREEQGASQHDDRASQDWDVVKAGQMMTASPSPGPGPRVAQKPALGRSTS
  LTEKDLKEAKARSQQIAAQLTTPPSSNSRGVQLFNRRRQRVNEFTLESHGQRGQKPSQESLRVLPSSLPG
  HAPGLSLSSTSLPEPGPPRHPSPQSPDRGVPGHSMEGYSEEASLLRHLEKVASEEEEVPLVVYLKENAAL
  LTANGLHLSQNREAQQSSPAPPPAEVHSPAADVNQNLASPSATLTTPTSNSSHNPPATDVNQNPPATVVP
  QSLPLSSIQQNSSEAQLPSNGTGPASKPSTLCADGQPQAPAEEVRCSTLLIDKVSTPATTTSTFSREATL
  IPSSRPPASDFMSSSLLIDIQPNTLVVSADQEMSGRAAATTPTKVYSEVHFTLAKPPSVVNRTARPFGIQ
  APGGTSQMERSPMLERRHFGEKAPAPQPPSLPDRSPRPQRHIMSRSPMVERRMMGQRSPASERRPLGNFT
  APPTYTETLSTAPLASWVRSPPSYSVLYPSSDPKSSHLKGQAVPASKTGILEESMARRGSRKSMFTFVEK
  PKVTPNPDLLDLVQTADEKRRQRDQGEVGVEEEPFALGAEASNFQQEPAPRDRASPAAAEEVVPEWASCL
  KSPRIQAKPKPKPNQNLSEASGKGAELYARRQSRMEKYVIESSSHTPELARCPSPTMSLPSSWKYPTNAP
  GAFRVASRSPARTPPASLYHGYLPENGVLRPEPTKQPPYQLRPSLFVLSPIKEPAKVSPRAASPAKPSSL
  DLVPNLPKGALPPSPALPRPSRSSPGLYTSPGQDSLQPTAVSPPYGGDISPVSPSRAWSPRAKQAPRPSF
  STRNAGIEAQVWKPSFCFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166209
• ORF Size: 2787 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166209.1, NP_001159681.1
• RefSeq Size: 5114 bp
• RefSeq ORF: 2790 bp
• Locus ID: 11346
• UniProt ID: Q8N3V7
• Cytogenetics: 5q33.1
• MW: 99.5 kDa
• Gene Summary: Synaptopodin is an actin-associated protein that may play a role in actin-based cell shape and motility. The name synaptopodin derives from the protein's associations with postsynaptic densities and dendritic spines and with renal podocytes (Mundel et al., 1997 [PubMed 9314539]).[supplied by OMIM, Mar 2008]