DISC1 (NM_001164537) Human Tagged ORF Clone

CAT#: RC228608

DISC1 (Myc-DDK-tagged)-Human disrupted in schizophrenia 1 (DISC1), transcript variant a

Product Images

Circular map for RC228608

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DISC1 (Cat# RC228608, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DISC1 antibody (Cat# TA890111). Positive lysates LY402701 (100ug) and LC402701 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DISC1
• Synonyms: C1orf136; SCZD9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228608 representing NM_001164537
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGGCGGGGGTCCTCAGGGCGCCCCAGCCGCCGCCGGCGGCGGCGGCGTGAGCCACCGCGCAGGCA
  GCCGGGATTGCTTACCACCTGCAGCGTGCTTTCGGAGGCGGCGGCTGGCACGGAGGCCGGGCTACATGAG
  AAGCTCGACAGGGCCTGGGATCGGGTTCCTTTCCCCAGCAGTGGGCACACTGTTCCGGTTCCCAGGAGGG
  GTGTCTGGCGAGGAGTCCCACCACTCGGAGTCCAGGGCCAGACAGTGTGGCCTTGACTCGAGAGGCCTCT
  TGGTCCGGAGCCCTGTTTCCAAGAGTGCAGCAGCCCCTACTGTGACCTCTGTGAGAGGAACCTCGGCGCA
  CTTTGGGATTCAGCTCAGAGGTGGCACCAGATTGCCTGACAGGCTTAGCTGGCCGTGTGGCCCTGGGAGT
  GCTGGGTGGCAGCAAGAGTTTGCAGCCATGGATAGTTCTGAGACCCTGGACGCCAGCTGGGAGGCAGCCT
  GCAGCGATGGAGCAAGGCGTGTCCGGGCAGCAGGCTCTCTGCCATCAGCAGAGTTGAGTAGCAACAGCTG
  CAGCCCTGGCTGTGGCCCTGAGGTCCCCCCAACCCCTCCTGGCTCTCACAGTGCCTTTACCTCAAGCTTT
  AGCTTTATTCGGCTCTCGCTTGGCTCTGCCGGGGAACGTGGAGAAGCAGAAGGCTGCCCACCATCCAGAG
  AGGCTGAGTCCCATTGCCAGAGCCCCCAGGAGATGGGAGCCAAAGCTGCCAGCTTGGACGGGCCTCACGA
  GGACCCGCGATGTCTCTCTCGGCCCTTCAGTCTCTTGGCTACACGGGTCTCTGCAGACTTGGCCCAGGCC
  GCAAGGAACAGCTCCAGGCCAGAGCGTGACATGCATTCTTTACCAGACATGGACCCTGGCTCCTCCAGTT
  CTCTGGATCCCTCACTGGCTGGCTGTGGTGGTGATGGGAGCAGCGGCTCAGGGGATGCCCACTCTTGGGA
  CACCCTGCTCAGGAAATGGGAGCCAGTGCTGCGGGACTGCCTGCTGAGAAACCGGAGGCAGATGGAGGTA
  ATATCCTTAAGATTAAAACTTCAGAAACTTCAGGAAGATGCAGTTGAGAATGATGATTATGATAAAGTAG
  AGACGGGGTTTCACTATGTTGGTCAAGCTGGTCTTGAACTCCTGACCTCAAGCAATCCACCTGCTTCGGC
  CTCCCAAAGTGCTGGGATTACAGCTGAGACGTTACAACAAAGATTAGAAGACCTGGAACAAGAGAAAATC
  AGCCTGCACTTTCAACTTCCTTCAAGGCAGCCAGCTCTTAGCAGTTTCCTGGGTCACCTGGCAGCACAAG
  TCCAGGCTGCCTTGCGCCGTGGGGCCACTCAGCAGGCCAGCGGAGATGACACCCACACCCCACTGAGAAT
  GGAGCCGAGGCTGTTGGAACCCACTGCTCAGGACAGCTTGCACGTGTCCATCACGAGACGAGACTGGCTT
  CTTCAGGAAAAGCAGCAGCTACAGAAAGAAATCGAAGCTCTCCAAGCAAGGATGTTTGTGCTGGAAGCCA
  AAGATCAACAGCTGAGAAGGGAAATAGAGGAGCAAGAGCAGCAACTCCAGTGGCAGGGCTGCGACCTGAC
  CCCACTGGTGGGCCAGCTGTCCCTGGGTCAGCTGCAGGAGGTCAGCAAGGCCTTGCAGGACACCCTGGCC
  TCAGCCGGTCAGATTCCCTTCCATGCAGAGCCACCGGAAACCATAAGGAGCCTCCAGGAAAGAATAAAAT
  CCCTCAACTTGTCACTTAAAGAAATCACTACTAAGGTGTGTATGAGTGAGAAATTCTGCAGCACCCTGAG
  GAAGAAAGTTAACGATATTGAAACCCAACTACCAGCCTTGCTTGAAGCCAAAATGCATGCCATATCAGGA
  AACCATTTCTGGACGGCTAAAGACCTCACCGAGGAGATTAGATCATTAACATCAGAGAGAGAAGGGCTGG
  AGGGACTCCTCAGCAAGCTGTTGGTGTTGAGTTCCAGGAATGTCAAAAAGCTGGGAAGTGTTAAAGAAGA
  TTACAACAGACTGAGAAGAGAAGTGGAGCACCAGGAGACTGCCTATGAAACAAGTGTGAAGGAAAATACT
  ATGAAGTACATGGAAACACTTAAGAATAAACTGTGCAGCTGCAAGTGTCCACTGCTTGGGAAAGTGTGGG
  AAGCTGACTTGGAAGCTTGTCGATTGCTTATCCAGAGCCTACAGCTCCAGGAAGCCAGGGGAAGCCTGTC
  TGTAGAAGATGAGAGGCAGATGGATGACTTAGAGGGAGCTGCTCCTCCTATTCCCCCCAGGCTCCACTCC
  GAGGATAAAAGGAAGACCCCTTTGAAGGTATTGGAAGAATGGAAGACTCACCTCATCCCCTCTCTGCACT
  GTGCTGGAGGTGAACAGAAAGAGGAATCTTACATCCTTTCTGCAGAACTTGGAGAAAAGTGTGAAGACAT
  AGGCAAGAAGCTATTGTACTTGGAAGATCAACTTCACACAGCAATCCACAGTCATGATGAAGATCTCATT
  CAGTCTCTCAGGAGGGAGCTCCAGATGGTGAAGGAAACTCTGCAGGCCATGATCCTGCAGCTCCAGCCAG
  CAAAGGAGGCGGGAGAAAGAGAAGCTGCAGCTTCCTGCATGACAGCTGGTGTCCACGAAGCACAAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228608 representing NM_001164537
  Red=Cloning site Green=Tags(s)
  
  MPGGGPQGAPAAAGGGGVSHRAGSRDCLPPAACFRRRRLARRPGYMRSSTGPGIGFLSPAVGTLFRFPGG
  VSGEESHHSESRARQCGLDSRGLLVRSPVSKSAAAPTVTSVRGTSAHFGIQLRGGTRLPDRLSWPCGPGS
  AGWQQEFAAMDSSETLDASWEAACSDGARRVRAAGSLPSAELSSNSCSPGCGPEVPPTPPGSHSAFTSSF
  SFIRLSLGSAGERGEAEGCPPSREAESHCQSPQEMGAKAASLDGPHEDPRCLSRPFSLLATRVSADLAQA
  ARNSSRPERDMHSLPDMDPGSSSSLDPSLAGCGGDGSSGSGDAHSWDTLLRKWEPVLRDCLLRNRRQMEV
  ISLRLKLQKLQEDAVENDDYDKVETGFHYVGQAGLELLTSSNPPASASQSAGITAETLQQRLEDLEQEKI
  SLHFQLPSRQPALSSFLGHLAAQVQAALRRGATQQASGDDTHTPLRMEPRLLEPTAQDSLHVSITRRDWL
  LQEKQQLQKEIEALQARMFVLEAKDQQLRREIEEQEQQLQWQGCDLTPLVGQLSLGQLQEVSKALQDTLA
  SAGQIPFHAEPPETIRSLQERIKSLNLSLKEITTKVCMSEKFCSTLRKKVNDIETQLPALLEAKMHAISG
  NHFWTAKDLTEEIRSLTSEREGLEGLLSKLLVLSSRNVKKLGSVKEDYNRLRREVEHQETAYETSVKENT
  MKYMETLKNKLCSCKCPLLGKVWEADLEACRLLIQSLQLQEARGSLSVEDERQMDDLEGAAPPIPPRLHS
  EDKRKTPLKVLEEWKTHLIPSLHCAGGEQKEESYILSAELGEKCEDIGKKLLYLEDQLHTAIHSHDEDLI
  QSLRRELQMVKETLQAMILQLQPAKEAGEREAAASCMTAGVHEAQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164537
• ORF Size: 2658 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164537.2
• RefSeq Size: 7165 bp
• RefSeq ORF: 2661 bp
• Locus ID: 27185
• Cytogenetics: 1q42.2
• MW: 96.8 kDa
• Gene Summary: This gene encodes a protein with multiple coiled coil motifs which is located in the nucleus, cytoplasm and mitochondria. The protein is involved in neurite outgrowth and cortical development through its interaction with other proteins. This gene is disrupted in a t(1;11)(q42.1;q14.3) translocation which segregates with schizophrenia and related psychiatric disorders in a large Scottish family. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]