EPB41 (NM_001166005) Human Tagged ORF Clone

CAT#: RC228600

EPB41 (Myc-DDK-tagged)-Human erythrocyte membrane protein band 4.1 (elliptocytosis 1, RH-linked) (EPB41), transcript variant 1

Product Images

Circular map for RC228600

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EPB41
• Synonyms: 4.1R; EL1; HE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228600 representing NM_001166005
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAACAGAGAAGAGTTTAGTGACTGAGGCCGAAAATTCACAGCACCAACAGAAGGAAGAGGGTGAGG
  AAGCCATAAACTCAGGCCAACAAGAACCTCAGCAGGAGGAATCTTGTCAAACAGCAGCTGAAGGAGATAA
  TTGGTGTGAACAGAAGCTGAAAGCTTCTAATGGAGACACTCCTACACATGAAGACTTGACCAAGAACAAG
  GAGCGGACATCAGAAAGCAGAGGACTTTCACGACTATTCTCCTCGTTTCTCAAAAGGCCCAAATCTCAGG
  TGTCCGAGGAAGAAGGCAAAGAAGTAGAGTCAGATAAAGAAAAAGGTGAAGGAGGTCAGAAAGAGATAGA
  ATTTGGAACCAGTCTTGATGAAGAGATCATTTTAAAGGCCCCAATTGCAGCTCCTGAACCGGAACTCAAA
  ACAGACCCATCTTTGGATCTTCATTCATTAAGCAGTGCAGAAACACAGCCTGCTCAGGAAGAACTCAGAG
  AAGATCCAGATTTTGAAATTAAGGAAGGAGAAGGACTTGAAGAGTGCTCCAAAATAGAAGTAAAAGAAGA
  AAGCCCTCAATCAAAAGCAGAAACAGAATTAAAAGCTTCCCAAAAACCAATCAGAAAACACAGGAACATG
  CACTGCAAGGTTTCTTTGTTGGATGACACAGTTTATGAATGTGTTGTGGAGAAACATGCTAAGGGACAAG
  ATTTGCTTAAACGAGTATGTGAGCATCTCAATCTTTTGGAAGAAGACTATTTTGGTCTAGCCATTTGGGA
  TAACGCAACCTCTAAGACATGGCTGGATTCCGCCAAAGAAATAAAAAAGCAGGTTCGTGGTGTCCCTTGG
  AATTTTACATTTAATGTAAAGTTTTATCCACCTGACCCAGCACAGTTAACAGAAGACATAACAAGATATT
  ATTTATGTCTTCAGCTTCGGCAGGACATAGTTGCAGGACGTCTGCCCTGTTCCTTTGCAACCTTAGCATT
  ATTAGGTTCTTACACCATCCAGTCTGAACTGGGAGACTACGACCCAGAACTCCATGGCGTGGATTATGTT
  AGTGATTTTAAACTGGCCCCGAATCAGACCAAGGAACTTGAAGAGAAGGTCATGGAACTGCATAAGTCAT
  ACAGGTCCATGACTCCAGCTCAGGCTGACTTGGAGTTTCTTGAGAATGCCAAAAAGTTGTCTATGTATGG
  AGTTGATCTTCATAAAGCAAAGGACTTGGAAGGAGTAGATATCATCCTAGGTGTCTGCTCTAGTGGCCTT
  CTGGTTTACAAAGATAAGCTGAGAATTAACCGCTTCCCTTGGCCCAAAGTGCTGAAGATTTCTTATAAAC
  GTAGTAGCTTTTTCATCAAGATTCGGCCTGGAGAGCAAGAGCAGTATGAAAGTACCATCGGATTCAAACT
  TCCCAGTTACCGAGCAGCTAAGAAATTATGGAAAGTCTGTGTAGAACATCACACGTTTTTCAGATTGACA
  TCTACAGACACCATTCCCAAAAGCAAATTTCTTGCGCTAGGATCCAAATTTCGATACAGTGGCCGGACTC
  AAGCTCAGACCAGGCAAGCTAGTGCTCTAATTGACAGGCCTGCCCCACACTTCGAGCGTACAGCAAGTAA
  ACGGGCGTCCCGGAGCCTCGATGGAGCAGCAGCTGTCGATTCGGCAGACCGAAGTCCTCGGCCCACTTCT
  GCACCTGCCATTACTCAGGGTCAGGTTGCAGAAGGTGGCGTCCTAGATGCCTCTGCTAAAAAAACAGTGG
  TCCCTAAAGCACAGAAGGAAACAGTGAAGGCTGAAGTGAAAAAGGAAGACGAGCCACCTGAGCAAGCTGA
  GCCAGAGCCCACAGAAGCATGGAAGGTGGAAAAAACCCACATCGAGGTGACAGTACCCACCTCAAATGGT
  GACCAAACACAGAAGCTTGCAGAAAAAACTGAAGATCTGATAAGAATGAGGAAGAAAAAGAGAGAAAGAC
  TAGATGGTGAAAACATTTATATCAGACATAGCAATTTAATGTTGGAGGATTTAGACAAGAGTCAAGAGGA
  GATCAAAAAACATCATGCCAGCATCAGTGAGCTGAAAAAGAACTTCATGGAGTCTGTACCAGAACCACGG
  CCTAGTGAATGGGATAAACGCTTATCCACTCACTCACCCTTCCGAACTCTTAACATCAATGGGCAAATCC
  CCACAGGAGAAGGACCTCCCCTGGTGAAGACACAAACTGTCACCATCTCAGATAATGCCAATGCTGTGAA
  AAGTGAAATCCCAACCAAAGACGTCCCTATTGTCCACACTGAGACCAAGACCATCACTTATGAGGCTGCC
  CAGACTGACGACAACAGTGGAGACTTGGACCCAGGAGTCTTGCTGACAGCTCAAACTATCACATCTGAGA
  CCCCAAGCAGCACCACCACAACTCAAATTACCAAGACTGTAAAAGGTGGGATTTCAGAGACACGTATTGA
  AAAGAGAATTGTGATCACAGGAGATGCTGATATTGACCATGATCAGGTCCTTGTACAAGCCATCAAGGAG
  GCAAAGGAGCAGCACCCAGACATGTCAGTGACCAAGGTGGTCGTCCACCAGGAGACCGAGATTGCTGATG
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228600 representing NM_001166005
  Red=Cloning site Green=Tags(s)
  
  MTTEKSLVTEAENSQHQQKEEGEEAINSGQQEPQQEESCQTAAEGDNWCEQKLKASNGDTPTHEDLTKNK
  ERTSESRGLSRLFSSFLKRPKSQVSEEEGKEVESDKEKGEGGQKEIEFGTSLDEEIILKAPIAAPEPELK
  TDPSLDLHSLSSAETQPAQEELREDPDFEIKEGEGLEECSKIEVKEESPQSKAETELKASQKPIRKHRNM
  HCKVSLLDDTVYECVVEKHAKGQDLLKRVCEHLNLLEEDYFGLAIWDNATSKTWLDSAKEIKKQVRGVPW
  NFTFNVKFYPPDPAQLTEDITRYYLCLQLRQDIVAGRLPCSFATLALLGSYTIQSELGDYDPELHGVDYV
  SDFKLAPNQTKELEEKVMELHKSYRSMTPAQADLEFLENAKKLSMYGVDLHKAKDLEGVDIILGVCSSGL
  LVYKDKLRINRFPWPKVLKISYKRSSFFIKIRPGEQEQYESTIGFKLPSYRAAKKLWKVCVEHHTFFRLT
  STDTIPKSKFLALGSKFRYSGRTQAQTRQASALIDRPAPHFERTASKRASRSLDGAAAVDSADRSPRPTS
  APAITQGQVAEGGVLDASAKKTVVPKAQKETVKAEVKKEDEPPEQAEPEPTEAWKVEKTHIEVTVPTSNG
  DQTQKLAEKTEDLIRMRKKKRERLDGENIYIRHSNLMLEDLDKSQEEIKKHHASISELKKNFMESVPEPR
  PSEWDKRLSTHSPFRTLNINGQIPTGEGPPLVKTQTVTISDNANAVKSEIPTKDVPIVHTETKTITYEAA
  QTDDNSGDLDPGVLLTAQTITSETPSSTTTTQITKTVKGGISETRIEKRIVITGDADIDHDQVLVQAIKE
  AKEQHPDMSVTKVVVHQETEIADE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166005
• ORF Size: 2592 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166005.2
• RefSeq ORF: 2595 bp
• Locus ID: 2035
• UniProt ID: P11171
• Cytogenetics: 1p35.3
• Protein Families: Druggable Genome
• Protein Pathways: Tight junction
• MW: 96.8 kDa
• Gene Summary: The protein encoded by this gene, together with spectrin and actin, constitute the red cell membrane cytoskeletal network. This complex plays a critical role in erythrocyte shape and deformability. Mutations in this gene are associated with type 1 elliptocytosis (EL1). Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Oct 2009]