eNOS (NOS3) (NM_001160111) Human Tagged ORF Clone

CAT#: RC228510

NOS3 (Myc-DDK-tagged)-Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 4

Product Images

Circular map for RC228510

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: eNOS
• Synonyms: ECNOS; eNOS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228510 representing NM_001160111
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAACTTGAAGAGCGTGGCCCAGGAGCCTGGGCCACCCTGCGGCCTGGGGCTGGGGCTGGGCCTTG
  GGCTGTGCGGCAAGCAGGGCCCAGCCACCCCGGCCCCTGAGCCCAGCCGGGCCCCAGCATCCCTACTCCC
  ACCAGCGCCAGAACACAGCCCCCCGAGCTCCCCGCTAACCCAGCCCCCAGAGGGGCCCAAGTTCCCTCGT
  GTGAAGAACTGGGAGGTGGGGAGCATCACCTATGACACCCTCAGCGCCCAGGCGCAGCAGGATGGGCCCT
  GCACCCCAAGACGCTGCCTGGGCTCCCTGGTATTTCCACGGAAACTACAGGGCCGGCCCTCCCCCGGCCC
  CCCGGCCCCTGAGCAGCTGCTGAGTCAGGCCCGGGACTTCATCAACCAGTACTACAGCTCCATTAAGAGG
  AGCGGCTCCCAGGCCCACGAACAGCGGCTTCAAGAGGTGGAAGCCGAGGTGGCAGCCACAGGCACCTACC
  AGCTTAGGGAGAGCGAGCTGGTGTTCGGGGCTAAGCAGGCCTGGCGCAACGCTCCCCGCTGCGTGGGCCG
  GATCCAGTGGGGGAAGCTGCAGGTGTTCGATGCCCGGGACTGCAGGTCTGCACAGGAAATGTTCACCTAC
  ATCTGCAACCACATCAAGTATGCCACCAACCGGGGCAACCTTCGCTCGGCCATCACAGTGTTCCCGCAGC
  GCTGCCCTGGCCGAGGAGACTTCCGAATCTGGAACAGCCAGCTGGTGCGCTACGCGGGCTACCGGCAGCA
  GGATGGCTCTGTGCGGGGGGACCCAGCCAACGTGGAGATCACCGAGCTCTGCATTCAGCACGGCTGGACC
  CCAGGAAACGGTCGCTTCGACGTGCTGCCCCTGCTGCTGCAGGCCCCAGATGATCCCCCAGAACTCTTCC
  TTCTGCCCCCCGAGCTGGTCCTTGAGGTGCCCCTGGAGCACCCCACGCTGGAGTGGTTTGCAGCCCTGGG
  CCTGCGCTGGTACGCCCTCCCGGCAGTGTCCAACATGCTGCTGGAAATTGGGGGCCTGGAGTTCCCCGCA
  GCCCCCTTCAGTGGCTGGTACATGAGCACTGAGATCGGCACGAGGAACCTGTGTGACCCTCACCGCTACA
  ACATCCTGGAGGATGTGGCTGTCTGCATGGACCTGGATACCCGGACCACCTCGTCCCTGTGGAAAGACAA
  GGCAGCAGTGGAAATCAACGTGGCCGTGCTGCACAGTTACCAGCTAGCCAAAGTCACCATCGTGGACCAC
  CACGCCGCCACGGCCTCTTTCATGAAGCACCTGGAGAATGAGCAGAAGGCCAGGGGGGGCTGCCCTGCAG
  ACTGGGCCTGGATCGTGCCCCCCATCTCGGGCAGCCTCACTCCTGTTTTCCATCAGGAGATGGTCAACTA
  TTTCCTGTCCCCGGCCTTCCGCTACCAGCCAGACCCCTGGAAGGGGAGTGCCGCCAAGGGCACCGGCATC
  ACCAGGAAGAAGACCTTTAAAGAAGTGGCCAACGCCGTGAAGATCTCCGCCTCGCTCATGGGCACGGTGA
  TGGCGAAGCGAGTGAAGGCGACAATCCTGTATGGCTCCGAGACCGGCCGGGCCCAGAGCTACGCACAGCA
  GCTGGGGAGACTCTTCCGGAAGGCTTTTGATCCCCGGGTCCTGTGTATGGATGAGTATGACGTGGTGTCC
  CTCGAACACGAGACGCTGGTGCTGGTGGTAACCAGCACATTTGGGAATGGGGATCCCCCGGAGAATGGAG
  AGGGTCTCACTTTGTGGCCCAGGCTGGAGTGCAGTAGTACAATCACGGCTCACTGCAGCCTCAACCTCCT
  AGACTCAAGCAATCCTCCCACTTCAACCTCCCAAGTAGTTGGGACTACAGGCGCATGCCATGATGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228510 representing NM_001160111
  Red=Cloning site Green=Tags(s)
  
  MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPR
  VKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKR
  SGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTY
  ICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWT
  PGNGRFDVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPA
  APFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDH
  HAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGI
  TRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVS
  LEHETLVLVVTSTFGNGDPPENGEGLTLWPRLECSSTITAHCSLNLLDSSNPPTSTSQVVGTTGACHDA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001160111
• ORF Size: 1887 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001160111.1, NP_001153583.1
• RefSeq ORF: 1890 bp
• Locus ID: 4846
• UniProt ID: P29474
• Cytogenetics: 7q36.1
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer, VEGF signaling pathway
• MW: 68.8 kDa
• Gene Summary: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]