DISC1 (NM_001164555) Human Tagged ORF Clone

CAT#: RC228285

DISC1 (Myc-DDK-tagged)-Human disrupted in schizophrenia 1 (DISC1), transcript variant r

Product Images

Circular map for RC228285

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DISC1
• Synonyms: C1orf136; SCZD9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228285 representing NM_001164555
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGGCGGGGGTCCTCAGGGCGCCCCAGCCGCCGCCGGCGGCGGCGGCGTGAGCCACCGCGCAGGCA
  GCCGGGATTGCTTACCACCTGCAGCGTGCTTTCGGAGGCGGCGGCTGGCACGGAGGCCGGGCTACATGAG
  AAGCTCGACAGGGCCTGGGATCGGGTTCCTTTCCCCAGCAGTGGGCACACTGTTCCGGTTCCCAGGAGGG
  GTGTCTGGCGAGGAGTCCCACCACTCGGAGTCCAGGGCCAGACAGTGTGGCCTTGACTCGAGAGGCCTCT
  TGGTCCGGAGCCCTGTTTCCAAGAGTGCAGCAGCCCCTACTGTGACCTCTGTGAGAGGAACCTCGGCGCA
  CTTTGGGATTCAGCTCAGAGGTGGCACCAGATTGCCTGACAGGCTTAGCTGGCCGTGTGGCCCTGGGAGT
  GCTGGGTGGCAGCAAGAGTTTGCAGCCATGGATAGTTCTGAGACCCTGGACGCCAGCTGGGAGGCAGCCT
  GCAGCGATGGAGCAAGGCGTGTCCGGGCAGCAGGCTCTCTGCCATCAGCAGAGTTGAGTAGCAACAGCTG
  CAGCCCTGGCTGTGGCCCTGAGGTCCCCCCAACCCCTCCTGGCTCTCACAGTGCCTTTACCTCAAGCTTT
  AGCTTTATTCGGCTCTCGCTTGGCTCTGCCGGGGAACGTGGAGAAGCAGAAGGCTGCCCACCATCCAGAG
  AGGCTGAGTCCCATTGCCAGAGCCCCCAGGAGATGGGAGCCAAAGCTGCCAGCTTGGACGGGCCTCACGA
  GGACCCGCGATGTCTCTCTCGGCCCTTCAGTCTCTTGGCTACACGGGTCTCTGCAGACTTGGCCCAGGCC
  GCAAGGAACAGCTCCAGGCCAGAGCGTGACATGCATTCTTTACCAGACATGGACCCTGGCTCCTCCAGTT
  CTCTGGATCCCTCACTGGCTGGCTGTGGTGGTGATGGGAGCAGCGGCTCAGGGGATGCCCACTCTTGGGA
  CACCCTGCTCAGGAAATGGGAGCCAGTGCTGCGGGACTGCCTGCTGAGAAACCGGAGGCAGATGGAGCTG
  AGACGTTACAACAAAGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228285 representing NM_001164555
  Red=Cloning site Green=Tags(s)
  
  MPGGGPQGAPAAAGGGGVSHRAGSRDCLPPAACFRRRRLARRPGYMRSSTGPGIGFLSPAVGTLFRFPGG
  VSGEESHHSESRARQCGLDSRGLLVRSPVSKSAAAPTVTSVRGTSAHFGIQLRGGTRLPDRLSWPCGPGS
  AGWQQEFAAMDSSETLDASWEAACSDGARRVRAAGSLPSAELSSNSCSPGCGPEVPPTPPGSHSAFTSSF
  SFIRLSLGSAGERGEAEGCPPSREAESHCQSPQEMGAKAASLDGPHEDPRCLSRPFSLLATRVSADLAQA
  ARNSSRPERDMHSLPDMDPGSSSSLDPSLAGCGGDGSSGSGDAHSWDTLLRKWEPVLRDCLLRNRRQMEL
  RRYNKD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164555
• ORF Size: 1068 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164555.1, NP_001158027.1
• RefSeq ORF: 1071 bp
• Locus ID: 27185
• UniProt ID: Q9NRI5
• Cytogenetics: 1q42.2
• MW: 37 kDa
• Gene Summary: This gene encodes a protein with multiple coiled coil motifs which is located in the nucleus, cytoplasm and mitochondria. The protein is involved in neurite outgrowth and cortical development through its interaction with other proteins. This gene is disrupted in a t(1;11)(q42.1;q14.3) translocation which segregates with schizophrenia and related psychiatric disorders in a large Scottish family. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]