MNX1 (NM_001165255) Human Tagged ORF Clone

CAT#: RC228123

MNX1 (Myc-DDK-tagged)-Human motor neuron and pancreas homeobox 1 (MNX1), transcript variant 2

Product Images

Circular map for RC228123

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MNX1
• Synonyms: HB9; HLXB9; HOXHB9; SCRA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228123 representing NM_001165255
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGGACTCTCAACAGTAGGTGCCTGCCCTGGAATCCTGGGCGCCCAACAAGCCCAGGCGCAGTCGA
  ACCTCCTGGGGAAGTGCCGCCGGCCGCGCACCGCCTTCACCAGCCAGCAGCTGCTGGAGCTGGAGCACCA
  GTTCAAGCTCAACAAGTACCTGTCGCGGCCCAAGCGCTTCGAGGTGGCCACCTCGCTCATGCTCACCGAG
  ACCCAGGTGAAGATTTGGTTCCAGAACCGGCGGATGAAATGGAAACGCAGCAAAAAGGCCAAAGAGCAGG
  CGGCGCAGGAAGCGGAGAAACAGAAGGGCGGCGGCGGGGGCGCGGGGAAGGGCGGCGCGGAGGAGCCGGG
  AGCCGAGGAGCTGCTGGGGCCGCCAGCGCCCGGAGACAAGGGCAGCGGACGCCGCCTGCGGGACTTGAGG
  GACAGTGACCCCGAGGAGGACGAGGACGAGGACGACGAGGACCATTTCCCCTACAGCAACGGCGCCAGCG
  TCCACGCCGCCTCCTCCGACTGCTCCTCGGAGGACGACTCGCCGCCCCCGCGGCCCAGCCACCAGCCCGC
  GCCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228123 representing NM_001165255
  Red=Cloning site Green=Tags(s)
  
  MGGLSTVGACPGILGAQQAQAQSNLLGKCRRPRTAFTSQQLLELEHQFKLNKYLSRPKRFEVATSLMLTE
  TQVKIWFQNRRMKWKRSKKAKEQAAQEAEKQKGGGGGAGKGGAEEPGAEELLGPPAPGDKGSGRRLRDLR
  DSDPEEDEDEDDEDHFPYSNGASVHAASSDCSSEDDSPPPRPSHQPAPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001165255
• ORF Size: 567 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001165255.1, NP_001158727.1
• RefSeq ORF: 570 bp
• Locus ID: 3110
• UniProt ID: P50219
• Cytogenetics: 7q36.3
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Maturity onset diabetes of the young
• MW: 20.4 kDa
• Gene Summary: This gene encodes a nuclear protein, which contains a homeobox domain and is a transcription factor. Mutations in this gene result in Currarino syndrome, an autosomic dominant congenital malformation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]