FNDC7 (NM_001144937) Human Tagged ORF Clone

CAT#: RC227901

FNDC7 (Myc-DDK-tagged)-Human fibronectin type III domain containing 7 (FNDC7)

Product Images

Circular map for RC227901

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FNDC7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227901 representing NM_001144937
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGTGGACGAGAGACATGTTTGCCTTTGATTGGATTCATTCTTATCTGTCTTAAAATGGTTGCTT
  CAGCAAAATCAGCTCCTGAAATACCCACTATTGATCAGGCATATTCAAAACTCAGCAACAGTATCACTGT
  AGAATGGGCTACAGTGCCGGGTGCCACCAGTTACCTCCTCACGGCTGAAGACGGGGACACAGTCATTGAA
  ACCACGGTGGCCAATTCCCCAGGCACTGTGACGGGACTAAAGGCTGCAACCTGGTATGAAATCACCATCA
  GATCCATCAGCGCTGCTGGGAGAAGCCAGGCGTCACCTCCAAAGCAGGCAAAGACAGTGTTGGCTGCACC
  AATTCTAGAAGTAAGCTCTCCAAGTTCAGACTCCATTCTTGTGCAGTGGGAAGCTGTATATATGGCAATT
  GCATTCTCCGTGTCCATTATGCGAGCCAATGGCTTGGGGAGTATATGGAAAGAGAATACTACAAACACCT
  CCTTGACATTCACCAGTTTAGAAGCCGGAACTCTCTACACCATAAAGGCCTATGCATGGAATGCCAACAG
  AATCCCTGGGGATGACTCCACCTGCAATCAGAGAACAAGTCCTCGGGCCCCTGCCAACATTCAAGTCTCT
  TTCGATAGTGGAGCTCTGAAGGCATCTTTTTCCTGGGCACGGGCAGAAGGAGCTTTCAATTATACTGTGA
  TGGCTTTGAGCGACTCTTCAGAGCTGACCTGCAGTACAACTTTCAGTTCCTGCACCATCTCTTCCCTCCA
  GTGTGGAACTGAATACTTGATTTCAGTTTTAGCAAGTAATGATGCTGGATCTAGCAAATCATCTTCAGCA
  ATGACCCTGAAAACTGTTGCTTGTGCACCCGGAAGAGTGACGATCCAAGAAGATCCCCCTGGCCACCTGT
  CTGTGGCTTGGTCCAGTGTAGATCTGGGTGACTACTATGTGGTCTTTGTGAAGAGTGATGATGGCTTGGA
  AGTACACTGCAACACATCTCTCACTCAGTGCAACTTCTTATCTGAGTGTGGCTTCACTTATTTTATTAGT
  GTTTTTGTCTATAACAAGGCAGGGCAAAGTCCTTTGGGTGACATATTCAATTATACCACAGCTCCCTGTT
  GTCCTAGTGACATTAACCCCGTGTTGGTGTCCAGTGACAGAGTTGAGATTGTCTGGTCTCCTGTCCGTGG
  TGCCGAACTGTATGAAACCAAGGCTGTAGATGGGTACAACATGGTTGAGTGCAATGACACTACTCCTGCG
  TGCACCCTTTCGGCTCTAGAGTGTGACACCAAGTACAACATCACAGTGTATTCATTCAATGAAGTCCGAG
  GCAGCAATATGTCATGTACTCCCCAGTTCATAACCACAGCTCCTTGCAGTCCTGAAATAAAAAATGTTTC
  AAGGGATGCATTCTCCATGATTAATGTGCACTGGCGATCCACTAATGATGATGCTACTTACACGGTGACT
  GCCCAAGGGGAGAAAGGACTGTATCAGTGCAGCAGCACAGGAGAGTCCTGCACCATGCGGGGCTTGCCCT
  GTGGCTCAGTGTTCTCTGTCACTGCTGTGGCCGAAACACAGGCAGGACGGAGCCTGCCCAGCTACAGTGT
  GCCCCTGGAAACAGTGCCATGCTGTCCAACCGGTCTGACAGTAACTCAAATCACCCAGTCAGTAATCAAC
  GTGAGCTGGACTATTGGGAGAGTGGCTCAAACCCATGTTGCAGTTCTGGAGTCACACACTGGACAGTCTA
  AGTGTCACACTCATCAAAACCACTGCCTCCTAGGATGCATCACATGTGGCATCAATTACACAGTGACATT
  AAAAGCAATTAGTGCCACCGGGTTGACTGCAGATTGCTCCTACCAAAGTTATTTCTCTGGTGCCTGCTGC
  CCTTTGGGGGTGAAATTATATAGGCTGGGCCCTAATGGCATCCGGATCTACTGGCAAGCCTCCAGGGGCT
  CTGCCAATTACAGCACTGACCTCTATGGCTCCAAAGGCATTTTCACGTGCACCCCGAGTGCTGGCCTCAG
  TTTCTGTGATGTCACTGAGATACCCTGTGGGGATGTATACACTGTGATGGTCTCACCAGTTGCTAAAACA
  GGATTGAAGCTTACTTTCTGTCCAAAAAAAATATATTCAGTAACTTGCTCTGGAAGTACACTTGGAATGG
  TGATTTATAGAGGGAAGAGAAATGAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227901 representing NM_001144937
  Red=Cloning site Green=Tags(s)
  
  MAGGRETCLPLIGFILICLKMVASAKSAPEIPTIDQAYSKLSNSITVEWATVPGATSYLLTAEDGDTVIE
  TTVANSPGTVTGLKAATWYEITIRSISAAGRSQASPPKQAKTVLAAPILEVSSPSSDSILVQWEAVYMAI
  AFSVSIMRANGLGSIWKENTTNTSLTFTSLEAGTLYTIKAYAWNANRIPGDDSTCNQRTSPRAPANIQVS
  FDSGALKASFSWARAEGAFNYTVMALSDSSELTCSTTFSSCTISSLQCGTEYLISVLASNDAGSSKSSSA
  MTLKTVACAPGRVTIQEDPPGHLSVAWSSVDLGDYYVVFVKSDDGLEVHCNTSLTQCNFLSECGFTYFIS
  VFVYNKAGQSPLGDIFNYTTAPCCPSDINPVLVSSDRVEIVWSPVRGAELYETKAVDGYNMVECNDTTPA
  CTLSALECDTKYNITVYSFNEVRGSNMSCTPQFITTAPCSPEIKNVSRDAFSMINVHWRSTNDDATYTVT
  AQGEKGLYQCSSTGESCTMRGLPCGSVFSVTAVAETQAGRSLPSYSVPLETVPCCPTGLTVTQITQSVIN
  VSWTIGRVAQTHVAVLESHTGQSKCHTHQNHCLLGCITCGINYTVTLKAISATGLTADCSYQSYFSGACC
  PLGVKLYRLGPNGIRIYWQASRGSANYSTDLYGSKGIFTCTPSAGLSFCDVTEIPCGDVYTVMVSPVAKT
  GLKLTFCPKKIYSVTCSGSTLGMVIYRGKRNEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001144937
• ORF Size: 2199 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001144937.3
• RefSeq ORF: 2202 bp
• Locus ID: 163479
• UniProt ID: Q5VTL7
• Cytogenetics: 1p13.3
• MW: 78.1 kDa