SH2B1 (NM_001145795) Human Tagged ORF Clone

CAT#: RC227881

SH2B1 (Myc-DDK-tagged)-Human SH2B adaptor protein 1 (SH2B1), transcript variant 1

Product Images

Circular map for RC227881

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  Western blot validation of overexpression lysate (Cat# LY429009) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227881 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SH2B1 protein (Cat# TP327881). The protein was produced from HEK293T cells transfected with SH2B1 cDNA clone (Cat# RC227881) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SH2B1
• Synonyms: PSM; SH2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227881 representing NM_001145795
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGTGCCCCTTCCCCAGAGGACGGGGCCTCCCCCTCGTCTCCCCCGCTGCCCCCACCCCCGCCCC
  CTAGTTGGCGGGAGTTCTGTGAGTCCCACGCCCGGGCTGCGGCTCTGGACTTTGCCCGCCGTTTTCGCCT
  CTACCTGGCCTCCCACCCCCAATATGCGGGGCCCGGGGCCGAGGCTGCCTTCTCCCGCCGTTTTGCTGAG
  CTCTTCCTGCAGCACTTTGAAGCCGAGGTGGCCCGGGCCTCTGGCTCCCTGTCGCCACCCATCCTGGCTC
  CCCTGAGCCCTGGTGCGGAGATTTCGCCACATGACCTGTCCCTTGAGAGCTGCAGGGTGGGTGGGCCCCT
  GGCTGTGCTGGGCCCTTCTCGATCATCTGAGGACCTGGCCGGCCCCCTCCCTTCCTCAGTCTCTTCCTCC
  TCTACAACCTCCTCCAAGCCGAAGCTCAAGAAGCGCTTTTCCCTGCGTTCAGTGGGTCGCTCTGTCCGAG
  GCTCAGTCCGTGGCATCCTGCAGTGGCGGGGGACCGTTGACCCTCCCTCCTCCGCTGGGCCCCTGGAGAC
  CTCGTCAGGCCCCCCAGTCTTAGGTGGAAACAGCAACTCCAACTCCTCTGGCGGGGCTGGGACCGTTGGT
  AGGGGACTGGTCAGTGATGGAACGTCCCCTGGGGAAAGATGGACTCACCGTTTTGAGAGGCTGAGACTCA
  GTCGGGGAGGGGGCGCCTTGAAGGATGGAGCAGGGATGGTGCAGAGGGAAGAGCTGCTGAGTTTCATGGG
  GGCTGAGGAGGCAGCCCCTGACCCAGCCGGAGTGGGCCGGGGAGGAGGGGTGGCTGGGCCTCCTTCAGGG
  GGAGGAGGGCAGCCTCAGTGGCAGAAGTGTCGCCTGCTGCTTCGAAGTGAAGGAGAAGGAGGAGGAGGAA
  GTCGCCTGGAGTTCTTTGTACCACCCAAGGCCTCTCGGCCCCGACTCAGCATCCCCTGCTCTTCTATCAC
  AGACGTCCGGACAACCACAGCCCTGGAGATGCCTGACCGGGAGAACACGTTTGTGGTTAAGGTGGAAGGT
  CCATCCGAGTATATCATGGAGACAGTGGATGCCCAGCATGTGAAGGCCTGGGTGTCTGACATCCAAGAAT
  GCCTGAGCCCAGGACCCTGCCCTGCTACCAGTCCCCGCCCCATGACCCTCCCTCTGGCCCCTGGGACCTC
  ATTCCTTACAAGGGAGAACACAGACAGCCTGGAGCTGTCCTGCCTGAATCACTCGGAGAGTCTACCCAGC
  CAGGACCTGCTGCTTGGACCCAGCGAGAGCAATGACCGCCTGTCGCAGGGGGCATATGGGGGCCTCTCAG
  ACCGCCCCTCGGCATCCATCTCCCCCAGCTCTGCCTCCATTGCCGCCTCCCATTTTGACTCGATGGAACT
  GCTTCCCCCAGAGTTGCCCCCCCGCATCCCCATTGAAGAGGGACCCCCAACAGGGACAGTTCATCCCCTC
  TCAGCCCCCTACCCTCCCTTGGACACTCCGGAAACAGCCACAGGGTCCTTCCTGTTCCAGGGGGAGCCAG
  AGGGCGGTGAGGGGGACCAGCCCCTCTCAGGGTATCCTTGGTTCCACGGGATGCTCTCTCGGCTCAAGGC
  TGCACAGTTGGTGCTGACTGGCGGCACTGGCTCCCACGGTGTCTTCCTGGTGCGCCAGAGTGAGACAAGG
  CGGGGTGAATACGTCCTCACCTTCAACTTCCAGGGCAAGGCCAAGCACCTGCGTTTGTCGCTGAACGAGG
  AGGGTCAGTGCCGGGTCCAGCACCTGTGGTTCCAGTCCATTTTCGATATGCTCGAGCACTTCCGGGTGCA
  CCCCATCCCTTTGGAGTCGGGAGGCTCCAGTGATGTTGTCCTTGTCAGCTATGTCCCATCCTCCCAGCGA
  CAGCAGGAACCGACCACCTCCCATGACCCACCCCAGCCCCCTGAACCCCCTTCATGGACAGATCCCCCAC
  AGCCTGGGGCAGAAGAGGCGTCGAGGGCGCCAGAAGTGGCGGCAGCAGCAGCCGCAGCAGCCAAAGAGAG
  GCAAGAGAAAGAGAAAGCGGGCGGTGGAGGGGTCCCGGAAGAGCTGGTCCCCGTGGTTGAGCTGGTCCCC
  GTGGTTGAATTGGAAGAGGCCATAGCCCCAGGCTCAGAGGCCCAGGGCGCTGGGTCTGGTGGGGACGCGG
  GGGTGCCCCCAATGGTGCAGCTGCAGCAGTCACCACTAGGGGGTGATGGAGAGGAAGGGGGCCACCCCAG
  GGCCATTAACAACCAGTACTCCTTCGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227881 representing NM_001145795
  Red=Cloning site Green=Tags(s)
  
  MNGAPSPEDGASPSSPPLPPPPPPSWREFCESHARAAALDFARRFRLYLASHPQYAGPGAEAAFSRRFAE
  LFLQHFEAEVARASGSLSPPILAPLSPGAEISPHDLSLESCRVGGPLAVLGPSRSSEDLAGPLPSSVSSS
  STTSSKPKLKKRFSLRSVGRSVRGSVRGILQWRGTVDPPSSAGPLETSSGPPVLGGNSNSNSSGGAGTVG
  RGLVSDGTSPGERWTHRFERLRLSRGGGALKDGAGMVQREELLSFMGAEEAAPDPAGVGRGGGVAGPPSG
  GGGQPQWQKCRLLLRSEGEGGGGSRLEFFVPPKASRPRLSIPCSSITDVRTTTALEMPDRENTFVVKVEG
  PSEYIMETVDAQHVKAWVSDIQECLSPGPCPATSPRPMTLPLAPGTSFLTRENTDSLELSCLNHSESLPS
  QDLLLGPSESNDRLSQGAYGGLSDRPSASISPSSASIAASHFDSMELLPPELPPRIPIEEGPPTGTVHPL
  SAPYPPLDTPETATGSFLFQGEPEGGEGDQPLSGYPWFHGMLSRLKAAQLVLTGGTGSHGVFLVRQSETR
  RGEYVLTFNFQGKAKHLRLSLNEEGQCRVQHLWFQSIFDMLEHFRVHPIPLESGGSSDVVLVSYVPSSQR
  QQEPTTSHDPPQPPEPPSWTDPPQPGAEEASRAPEVAAAAAAAAKERQEKEKAGGGGVPEELVPVVELVP
  VVELEEAIAPGSEAQGAGSGGDAGVPPMVQLQQSPLGGDGEEGGHPRAINNQYSFV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145795
• ORF Size: 2268 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145795.1, NP_001139267.1
• RefSeq ORF: 2271 bp
• Locus ID: 25970
• UniProt ID: Q9NRF2
• Cytogenetics: 16p11.2
• Protein Pathways: Neurotrophin signaling pathway
• MW: 79.2 kDa
• Gene Summary: This gene encodes a member of the SH2-domain containing mediators family. The encoded protein mediates activation of various kinases and may function in cytokine and growth factor receptor signaling and cellular transformation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009]