DACH2 (NM_001139515) Human Tagged ORF Clone

CAT#: RC227811

DACH2 (Myc-DDK-tagged)-Human dachshund homolog 2 (Drosophila) (DACH2), transcript variant 3

Product Images

Circular map for RC227811

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  Western blot validation of overexpression lysate (Cat# LY427976) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227811 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DACH2 protein (Cat# TP327811). The protein was produced from HEK293T cells transfected with DACH2 cDNA clone (Cat# RC227811) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DACH2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227811 representing NM_001139515
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAGAAAACAAGCTGTTAACAGTTCAAGACCCGGCAGGCCCCCTAAGCGTTCTTTGGGAGTGTTGC
  AGGAAAATGCCCGCCTTCTGACCCATGCAGTCCCAGGCCTCTTATCGCCAGGACTTATCACTCCGACAGG
  TATAACAGCTGCAGCGATGGCTGAGGCGATGAAACTTCAGAAGATGAAGCTTATGGCTATGAACACTCTT
  CAGGGAAATGGAAGCCAAAATGGGACCGAATCAGAGCCTGATGATCTTAATTCTAACACAGGTGGAAGTG
  AATCCTCCTGGGATAAAGATAAGATGCAGTCTCCATTTGCTGCACCTGGACCCCAACATGGAATTGCTCA
  TGCAGCCCTAGCTGGCCAGCCAGGCATTGGGGGTGCTCCAACCCTCAATCCACTGCAGCAGAACCACCTG
  CTAACCAATAGACTGGATCTGCCATTTATGATGATGCCTCATCCCCTACTTCCAGTCAGCTTACCTCCTG
  CATCAGTTGCCATGGCAATGAATCAGATGAACCATCTCAATACTATTGCCAACATGGCTGCTGCAGCACA
  GATTCACAGTCCACTCTCCAGAGCTGGTACCTCTGTTATAAAGGAGCGGATCCCAGAGAGTCCTTCTCCT
  GCTCCTTCTCTAGAAGAGAATCATCGTCCTGGGAGCCAGACCTCTTCCCACACCAGCAGCAGTGTGTCCA
  GCTCTCCCTCTCAGATGGATCATCATTTGGAAAGAATGGAAGAGGTACCAGTTCAAATTCCAATAATGAA
  GTCACCCTTGGACAAGATACAGCTGACTCCTGGGCAGGCATTGCCCGCTGGATTCCCTGGACCATTCATT
  TTTGCTGATAGTCTGTCCTCCGTGGAGACTCTGTTGACCAACATTCAGGGTCTGCTGAAAGTTGCTTTGG
  ATAATGCTCGCATCCAGGAGAAGCAGATTCAACAAGAAAAGAAGGAGCTGCGACTGGAGCTCTATAGAGA
  GAGAGAAATTAGAGAAAACCTTGAAAGACAACTTGCAGTTGAGCTTCAAAGCAGAACTACTATGCAAAAG
  CGCCTGAAGAAGGAGAAAAAAACCAAGAGAAAATTGCAGGAAGCCTTGGAATTTGAATCAAAGCGCCGGG
  AGCAAGTGGAGCAGGCACTTAAGCAAGCCACCACTAGTGACAGTGGCCTGAGGATGTTAAAAGATACTGG
  AATTCCAGATATTGAAATAGAAAACAATGGGACTCCTCATGATAGTGCTGCTATGCAAGGAGGTAACTAT
  TACTGTTTAGAAATGGCACAACAGTTGTATTCAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227811 representing NM_001139515
  Red=Cloning site Green=Tags(s)
  
  MTRKQAVNSSRPGRPPKRSLGVLQENARLLTHAVPGLLSPGLITPTGITAAAMAEAMKLQKMKLMAMNTL
  QGNGSQNGTESEPDDLNSNTGGSESSWDKDKMQSPFAAPGPQHGIAHAALAGQPGIGGAPTLNPLQQNHL
  LTNRLDLPFMMMPHPLLPVSLPPASVAMAMNQMNHLNTIANMAAAAQIHSPLSRAGTSVIKERIPESPSP
  APSLEENHRPGSQTSSHTSSSVSSSPSQMDHHLERMEEVPVQIPIMKSPLDKIQLTPGQALPAGFPGPFI
  FADSLSSVETLLTNIQGLLKVALDNARIQEKQIQQEKKELRLELYREREIRENLERQLAVELQSRTTMQK
  RLKKEKKTKRKLQEALEFESKRREQVEQALKQATTSDSGLRMLKDTGIPDIEIENNGTPHDSAAMQGGNY
  YCLEMAQQLYSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001139515
• ORF Size: 1296 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001139515.1, NP_001132987.1
• RefSeq ORF: 1299 bp
• Locus ID: 117154
• UniProt ID: Q96NX9
• Cytogenetics: Xq21.2
• Protein Families: Transcription Factors
• MW: 47 kDa
• Gene Summary: This gene is one of two genes which encode a protein similar to the Drosophila protein dachshund, a transcription factor involved in cell fate determination in the eye, limb and genital disc of the fly. The encoded protein contains two characteristic dachshund domains: an N-terminal domain responsible for DNA binding and a C-terminal domain responsible for protein-protein interactions. This gene is located on the X chromosome and is subject to inactivation by DNA methylation. The encoded protein may be involved in regulation of organogenesis and myogenesis, and may play a role in premature ovarian failure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]