Dynamin 3 (DNM3) (NM_001136127) Human Tagged ORF Clone

CAT#: RC227774

DNM3 (Myc-DDK-tagged)-Human dynamin 3 (DNM3), transcript variant 2

Product Images

Circular map for RC227774

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dynamin 3
• Synonyms: Dyna III
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227774 representing NM_001136127
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAACCGGGAGATGGAGGAGCTGATCCCGCTGGTGAACCGTCTGCAGGACGCGTTTTCGGCGCTGG
  GACAGAGCTGCCTGCTGGAGCTGCCGCAGATCGCCGTGGTGGGCGGCCAGAGCGCCGGCAAGAGCTCGGT
  GCTCGAGAACTTCGTGGGCAGGGACTTTCTCCCTCGAGGGTCGGGCATTGTAACAAGACGACCTCTTGTG
  CTGCAGCTTGTTACTTCTAAAGCAGAATATGCCGAGTTTCTACATTGCAAAGGAAAGAAATTTACAGATT
  TTGATGAAGTTCGCCTTGAGATTGAAGCAGAAACAGATCGCGTGACTGGAATGAATAAAGGCATTTCCTC
  CATACCCATTAATTTACGAGTCTATTCCCCACACGTGTTAAATCTAACCCTTATTGATCTACCTGGAATA
  ACTAAAGTGCCTGTGGGAGATCAGCCACCAGATATCGAGTATCAGATCAGAGAAATGATTATGCAGTTCA
  TCACGAGGGAGAACTGTCTGATTTTAGCTGTTACTCCAGCCAACACTGATCTTGCAAACTCAGATGCGCT
  GAAGCTAGCTAAAGAAGTTGATCCTCAAGGTCTGAGAACCATTGGAGTTATCACCAAACTGGACCTTATG
  GATGAAGGAACGGATGCCAGGGATGTTCTAGAGAACAAACTGTTGCCTCTTCGCAGGGGTTACGTGGGGG
  TGGTAAACAGAAGCCAGAAGGACATAGATGGGAAGAAGGACATAAAGGCAGCCATGCTGGCAGAGAGGAA
  GTTTTTCCTTTCCCACCCGGCTTACAGACATATCGCTGACCGAATGGGAACCCCACACCTGCAGAAGGTC
  CTTAATCAGCAACTTACCAACCACATTCGGGATACCCTACCAAACTTCAGGAACAAACTACAGGGACAGT
  TGCTCTCCATAGAACATGAAGTAGAAGCCTACAAAAATTTCAAACCAGAAGACCCAACAAGGAAGACCAA
  AGCATTGCTGCAGATGGTTCAGCAATTTGCTGTGGACTTTGAGAAGAGAATTGAAGGGTCAGGGGATCAA
  GTAGATACCCTGGAACTCTCAGGTGGTGCTAAAATCAATCGTATTTTTCATGAACGCTTTCCTTTTGAGA
  TAGTAAAGATGGAGTTCAATGAGAAAGAATTGCGAAGAGAAATAAGCTATGCAATCAAAAACATACATGG
  TATCAGGACAGGGTTGTTTACTCCAGACATGGCATTTGAAGCGATAGTCAAGAAACAGATTGTAAAGTTG
  AAAGGGCCTTCCTTGAAGAGTGTGGATCTGGTAATACAAGAATTAATCAACACTGTGAAGAAGTGTACCA
  AAAAACTGGCAAACTTCCCCAGACTCTGCGAGGAAACGGAAAGGATTGTTGCTAACCACATTCGTGAGCG
  AGAAGGGAAGACAAAGGACCAGGTATTGCTATTGATTGACATTCAAGTCTCTTACATCAACACCAACCAT
  GAAGACTTCATTGGCTTCGCAAATGCTCAGCAGAGGAGCAGTCAGGTTCACAAGAAAACCACAGTTGGAA
  ATCAGGTGATTCGCAAGGGGTGGCTCACCATCAGCAACATTGGCATCATGAAAGGCGGCTCGAAGGGATA
  CTGGTTCGTCCTTACTGCGGAAAGCTTGTCCTGGTATAAAGATGATGAGGAAAAAGAAAAGAAGTACATG
  CTTCCCTTGGACAACCTGAAAGTTCGGGATGTGGAAAAGAGCTTTATGTCTAGCAAGCACATCTTTGCAC
  TCTTTAATACAGAGCAAAGGAATGTATACAAAGACTATCGCTTCCTTGAGCTGGCATGTGATTCCCAGGA
  GGATGTCGACAGCTGGAAGGCATCTCTACTAAGAGCTGGGGTCTATCCTGACAAATCTGTAGCTGAAAAT
  GATGAGAATGGACAAGCAGAAAACTTTTCCATGGACCCACAATTGGAGAGGCAAGTGGAGACCATTCGCA
  ACCTCGTAGACTCCTACATGTCCATTATCAACAAATGTATCCGAGATCTAATTCCAAAAACAATAATGCA
  CCTTATGATCAATAACGTTAAAGATTTCATAAATTCCGAGCTCCTAGCACAGTTGTATTCTTCAGAGGAC
  CAAAATACCCTGATGGAGGAATCTGCTGAGCAGGCTCAGCGCCGGGATGAGATGCTTCGAATGTATCAAG
  CACTGAAAGAAGCCCTTGGGATAATTGGGGACATCAGCACAGCCACCGTGTCCACTCCGGCACCCCCTCC
  AGTGGATGACTCCTGGATACAGCACTCTCGCAGGTCACCTCCTCCAAGCCCCACAACCCAAAGGAGGCCA
  ACACTAAGTGCTCCCCTCGCAAGGCCCACATCCGGCCGAGGACCAGCTCCTGCCATTCCCTCTCCTGGCC
  CCCACTCTGGGGCTCCTCCAGTCCCATTCCGTCCAGGCCCATTACCTCCTTTCCCCAGCAGCAGTGACTC
  CTTCGGAGCCCCTCCACAAGTTCCATCTAGGCCTACGAGGGCCCCGCCCAGTGTCCCAAGCCGGAGACCA
  CCCCCATCACCAACTCGTCCCACTATAATCCGCCCACTAGAATCCTCCCTGTTAGAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227774 representing NM_001136127
  Red=Cloning site Green=Tags(s)
  
  MGNREMEELIPLVNRLQDAFSALGQSCLLELPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLV
  LQLVTSKAEYAEFLHCKGKKFTDFDEVRLEIEAETDRVTGMNKGISSIPINLRVYSPHVLNLTLIDLPGI
  TKVPVGDQPPDIEYQIREMIMQFITRENCLILAVTPANTDLANSDALKLAKEVDPQGLRTIGVITKLDLM
  DEGTDARDVLENKLLPLRRGYVGVVNRSQKDIDGKKDIKAAMLAERKFFLSHPAYRHIADRMGTPHLQKV
  LNQQLTNHIRDTLPNFRNKLQGQLLSIEHEVEAYKNFKPEDPTRKTKALLQMVQQFAVDFEKRIEGSGDQ
  VDTLELSGGAKINRIFHERFPFEIVKMEFNEKELRREISYAIKNIHGIRTGLFTPDMAFEAIVKKQIVKL
  KGPSLKSVDLVIQELINTVKKCTKKLANFPRLCEETERIVANHIREREGKTKDQVLLLIDIQVSYINTNH
  EDFIGFANAQQRSSQVHKKTTVGNQVIRKGWLTISNIGIMKGGSKGYWFVLTAESLSWYKDDEEKEKKYM
  LPLDNLKVRDVEKSFMSSKHIFALFNTEQRNVYKDYRFLELACDSQEDVDSWKASLLRAGVYPDKSVAEN
  DENGQAENFSMDPQLERQVETIRNLVDSYMSIINKCIRDLIPKTIMHLMINNVKDFINSELLAQLYSSED
  QNTLMEESAEQAQRRDEMLRMYQALKEALGIIGDISTATVSTPAPPPVDDSWIQHSRRSPPPSPTTQRRP
  TLSAPLARPTSGRGPAPAIPSPGPHSGAPPVPFRPGPLPPFPSSSDSFGAPPQVPSRPTRAPPSVPSRRP
  PPSPTRPTIIRPLESSLLD
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136127
• ORF Size: 2577 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136127.3
• RefSeq ORF: 2580 bp
• Locus ID: 26052
• UniProt ID: Q9UQ16
• Cytogenetics: 1q24.3
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis
• MW: 96.5 kDa
• Gene Summary: This gene encodes a member of a family of guanosine triphosphate (GTP)-binding proteins that associate with microtubules and are involved in vesicular transport. The encoded protein functions in the development of megakaryocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]