ZNF447 (ZSCAN18) (NM_001145543) Human Tagged ORF Clone

CAT#: RC227637

ZSCAN18 (Myc-DDK-tagged)-Human zinc finger and SCAN domain containing 18 (ZSCAN18), transcript variant 4

Product Images

Circular map for RC227637

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  Western blot validation of overexpression lysate (Cat# LY428934) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227637 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ZSCAN18 protein (Cat# TP327637). The protein was produced from HEK293T cells transfected with ZSCAN18 cDNA clone (Cat# RC227637) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF447
• Synonyms: ZNF447
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227637 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCCTTTGGAGAAGGCGTTTGCCTCCCCCAGGAGCTCCCCAGCCCCGCCGGATCTGCCCACGCCGG
  GGTCAGCAGCCGGAGTCCAGCAGGAAGAACCCGAGACCATCCCTGAGAGGACCCCTGCTGACCTGGAGTT
  CTCCCGCCTGCGTTTCCGGGAATTTGTCTACCAGGAGGCTGCCGGGCCCCACCAGACCCTGGCCCGGCTG
  CATGAGCTGTGCCGCCAGTGGCTGATGCCTGAGGCGCGCTCCAAGGAGCAGATGCTGGAGCTGCTGGTGC
  TGGAGCAGTTCCTGGGCATCCTGCCTGATAAGGTCCGGCCCTGGGTGGTGGCACAGTACCCTGAGAGCTG
  CAAGAAGGCAGCCTCCCTGGTGGAGGGCCTCGCTGATGTCCTGGAAGAGCCAGGGATGCTGCTGGGCTCC
  CCTGCGGGCTCATCCTCAATTCTTAGCGATGGAGTGTACGAGAGGCACATGGACCCTCTGCTGCTACCAG
  GCGAGCTCGCGAGCCCCAGCCAGGCCCTTGGAGCTGGGGAGATCCCGGCACCTTCTGAGACACCCTGGCT
  TTCTCCGGACCCCCTGTTTCTGGAACAGAGGAGGGTCAGAGAAGCAAAGACCGAAGAGGACGGCCCTGCC
  AACACCGAGCAGAAGCTGAAGTCCTTTCCAGAGGACCCTCAGCACCTGGGGGAGTGGGGCCACCTGGACC
  CTGCCGAGGAGAACCTGAAGAGCTACCGGAAGCTGCTCCTGTGGGGGTATCAGCTTTCCCAGCCTGACGC
  TGCCTCCAGGCTGGACACTGAGGAACTCCGGTTGGTGGAAAGAGATCCACAAGGAAGCAGCCTCCCAGAA
  GGCGGGAGGCGGCAGGAGAGCGCTGGGTGCGCCTGCGAGGAGGCCGCCCCCGCGGGGGTGCTGCCTGAGC
  TGCCTACGGAGGCGCCCCCTGGGGACGCCCTTGCCGATCCCCCGTCGGGCACCACTGAGGAGGAGGAAGA
  GCAGCCTGGGAAGGCCCCGGACCCGCAGGACCCCCAGGACGCGGAGTCCGACTCTGCCACCGGATCGCAG
  AGGCAGTCCGTCATCCAGCAGCCTGCCCCGGACAGGGGCACGGCGAAACTGGGAACCAAGAGGCCGCACC
  CCGAGGATGGGGACGAGCAGAGCCTCGAGGGCGTCTCTAGCTCCGGCGACAGCGCAGGGCTGGAGGCCGG
  GCAGGGCCCTGGGGCTGACGAGCCGGGCTTGTCCCGCGGGAAGCCCTATGCCTGCGGCGAGTGCGGGGAG
  GCCTTCGCGTGGCTCTCGCACCTGATGGAGCACCACAGCAGCCATGGCGGCCGGAAGCGCTACGCCTGTC
  AGGGCTGCTGGAAGACCTTCCACTTCAGCCTGGCCCTAGCCGAGCACCAGAAGACCCACGAGAAGGAGAA
  AAGCTACGCGCTGGGGGGCGCCCGGGGCCCCCAACCGTCCACCCGCGAAGCCCAGGCGGGGGCTAGGGCG
  GGCGGTCCCCCAGAGAGCGTGGAGGGCGAGGCTCCCCCCGCACCCCCAGAGGCGCAGAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227637 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLPLEKAFASPRSSPAPPDLPTPGSAAGVQQEEPETIPERTPADLEFSRLRFREFVYQEAAGPHQTLARL
  HELCRQWLMPEARSKEQMLELLVLEQFLGILPDKVRPWVVAQYPESCKKAASLVEGLADVLEEPGMLLGS
  PAGSSSILSDGVYERHMDPLLLPGELASPSQALGAGEIPAPSETPWLSPDPLFLEQRRVREAKTEEDGPA
  NTEQKLKSFPEDPQHLGEWGHLDPAEENLKSYRKLLLWGYQLSQPDAASRLDTEELRLVERDPQGSSLPE
  GGRRQESAGCACEEAAPAGVLPELPTEAPPGDALADPPSGTTEEEEEQPGKAPDPQDPQDAESDSATGSQ
  RQSVIQQPAPDRGTAKLGTKRPHPEDGDEQSLEGVSSSGDSAGLEAGQGPGADEPGLSRGKPYACGECGE
  AFAWLSHLMEHHSSHGGRKRYACQGCWKTFHFSLALAEHQKTHEKEKSYALGGARGPQPSTREAQAGARA
  GGPPESVEGEAPPAPPEAQR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145543
• ORF Size: 1530 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145543.1, NP_001139015.1
• RefSeq Size: 2716 bp
• RefSeq ORF: 1533 bp
• Locus ID: 65982
• UniProt ID: Q8TBC5
• Cytogenetics: 19q13.43
• Protein Families: Transcription Factors
• MW: 54.9 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]