CD51 (ITGAV) (NM_001145000) Human Tagged ORF Clone

CAT#: RC227617

ITGAV (Myc-DDK-tagged)-Human integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) (ITGAV), transcript variant 3

Product Images

Circular map for RC227617

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CD51
• Synonyms: CD51; MSK8; VNRA; VTNR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227617 representing NM_001145000
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTTCCGCCGCGGCGACGGCTGCGCCTCGGTCCCCGCGGCCTCCCGCTTCTTCTCTCGGGACTCC
  TGCTACCTCTGTGCCGCGCCTTCAACCTAGACGTGGACAGTCCTGCCGAGTACTCTGGCCCCGAGGGAAG
  TTACTTCGGCTTCGCCGTGGATTTCTTCGTGCCCAGCGCGTCTTCCCGGATGTTTCTTCTCGTGGGAGCT
  CCCAAAGCAAACACCACCCAGCCTGGGATTGTGGAAGGAGGGCAGGTCCTCAAATGTGACTGGTCTTCTA
  CCCGCCGGTGCCAGCCAATTGAATTTGATGCAACAGGCAATAGAGATTATGCCAAGGATGATCCATTGGA
  ATTTAAGTCCCATCAGTGGTTTGGAGCATCTGTGAGGTCGAAACAGGATAAAATTTTGGCCTGTGCCCCA
  TTGTACCATTGGAGAACTGAGATGAAACAGGAGCGAGAGCCTGTTGGAACATGCTTTCTTCAAGATGGAA
  CAAAGACTGTTGAGTATGCTCCATGTAGATCACGTCAGCTTATTTCGGATCAAGTGGCAGAAATCGTATC
  TAAATACGACCCCAATGTTTACAGCATCAAGTATAATAACCAATTAGCAACTCGGACTGCACAAGCTATT
  TTTGATGACAGCTATTTGGGTTATTCTGTGGCTGTCGGAGATTTCAATGGTGATGGCATAGATGACTTTG
  TTTCAGGAGTTCCAAGAGCAGCAAGGACTTTGGGAATGGTTTATATTTATGATGGGAAGAACATGTCCTC
  CTTATACAATTTTACTGGCGAGCAGATGGCTGCATATTTCGGATTTTCTGTAGCTGCCACTGACATTAAT
  GGAGATGATTATGCAGATGTGTTTATTGGAGCACCTCTCTTCATGGATCGTGGCTCTGATGGCAAACTCC
  AAGAGGTGGGGCAGGTCTCAGTGTCTCTACAGAGAGCTTCAGGAGACTTCCAGACGACAAAGCTGAATGG
  ATTTGAGGTCTTTGCACGGTTTGGCAGTGCCATAGCTCCTTTGGGAGATCTGGACCAGGATGGTTTCAAT
  GATATTGCAATTGCTGCTCCATATGGGGGTGAAGATAAAAAAGGAATTGTTTATATCTTCAATGGAAGAT
  CAACAGGCTTGAACGCAGTCCCATCTCAAATCCTTGAAGGGCAGTGGGCTGCTCGAAGCATGCCACCAAG
  CTTTGGCTATTCAATGAAAGGAGCCACAGATATAGACAAAAATGGATATCCAGACTTAATTGTAGGAGCT
  TTTGGTGTAGATCGAGCTATCTTATACAGGGCCAGACCAGTTATCACTGTAAATGCTGGTCTTGAAGTGT
  ACCCTAGCATTTTAAATCAAGACAATAAAACCTGCTCACTGCCTGGAACAGCTCTCAAAGTTTCCTGTTT
  TAATGTTAGGTTCTGCTTAAAGGCAGATGGCAAAGGAGTACTTCCCAGGAAACTTAATTTCCAGGTGGAA
  CTTCTTTTGGATAAACTCAAGCAAAAGGGAGCAATTCGACGAGCACTGTTTCTCTACAGCAGGTCCCCAA
  GTCACTCCAAGAACATGACTATTTCAAGGGGGGGACTGATGCAGTGTGAGGAATTGATAGCGTATCTGCG
  GGATGAATCTGAATTTAGAGACAAACTCACTCCAATTACTATTTTTATGGAATATCGGTTGGATTATAGA
  ACAGCTGCTGATACAACAGGCTTGCAACCCATTCTTAACCAGTTCACGCCTGCTAACATTAGTCGACAGG
  CTCACATTCTACTTGACTGTGGTGAAGACAATGTCTGTAAACCCAAGCTGGAAGTTTCTGTAGATAGTGA
  TCAAAAGAAGATCTATATTGGGGATGACAACCCTCTGACATTGATTGTTAAGGCTCAGAATCAAGGAGAA
  GGTGCCTACGAAGCTGAGCTCATCGTTTCCATTCCACTGCAGGCTGATTTCATCGGGGTTGTCCGAAACA
  ATGAAGCCTTAGCAAGACTTTCCTGTGCATTTAAGACAGAAAACCAAACTCGCCAGGTGGTATGTGACCT
  TGGAAACCCAATGAAGGCTGGAACTCAACTCTTAGCTGGTCTTCGTTTCAGTGTGCACCAGCAGTCAGAG
  ATGGATACTTCTGTGAAATTTGACTTACAAATCCAAAGCTCAAATCTATTTGACAAAGTAAGCCCAGTTG
  TATCTCACAAAGTTGATCTTGCTGTTTTAGCTGCAGTTGAGATAAGAGGAGTCTCGAGTCCTGATCATAT
  CTTTCTTCCGATTCCAAACTGGGAGCACAAGGAGAACCCTGAGACTGAAGAAGATGTTGGGCCAGTTGTT
  CAGCACATCTATGAGCTGAGAAACAATGGTCCAAGTTCATTCAGCAAGGCAATGCTCCATCTTCAGTGGC
  CTTACAAATATAATAATAACACTCTGTTGTATATCCTTCATTATGATATTGATGGACCAATGAACTGCAC
  TTCAGATATGGAGATCAACCCTTTGAGAATTAAGATCTCATCTTTGCAAACAACTGAAAAGAATGACACG
  GTTGCCGGGCAAGGTGAGCGGGACCATCTCATCACTAAGCGGGATCTTGCCCTCAGTGAAGGAGATATTC
  ACACTTTGGGTTGTGGAGTTGCTCAGTGCTTGAAGATTGTCTGCCAAGTTGGGAGATTAGACAGAGGAAA
  GAGTGCAATCTTGTACGTAAAGTCATTACTGTGGACTGAGACTTTTATGAATAAAGAAAATCAGAATCAT
  TCCTATTCTCTGAAGTCGTCTGCTTCATTTAATGTCATAGAGTTTCCTTATAAGAATCTTCCAATTGAGG
  ATATCACCAACTCCACATTGGTTACCACTAATGTCACCTGGGGCATTCAGCCAGCGCCCATGCCTGTGCC
  TGTGTGGGTGATCATTTTAGCAGTTCTAGCAGGATTGTTGCTACTGGCTGTTTTGGTATTTGTAATGTAC
  AGGATGGGCTTTTTTAAACGGGTCCGGCCACCTCAAGAAGAACAAGAAAGGGAGCAGCTTCAACCTCATG
  AAAATGGTGAAGGAAACTCAGAAACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227617 representing NM_001145000
  Red=Cloning site Green=Tags(s)
  
  MAFPPRRRLRLGPRGLPLLLSGLLLPLCRAFNLDVDSPAEYSGPEGSYFGFAVDFFVPSASSRMFLLVGA
  PKANTTQPGIVEGGQVLKCDWSSTRRCQPIEFDATGNRDYAKDDPLEFKSHQWFGASVRSKQDKILACAP
  LYHWRTEMKQEREPVGTCFLQDGTKTVEYAPCRSRQLISDQVAEIVSKYDPNVYSIKYNNQLATRTAQAI
  FDDSYLGYSVAVGDFNGDGIDDFVSGVPRAARTLGMVYIYDGKNMSSLYNFTGEQMAAYFGFSVAATDIN
  GDDYADVFIGAPLFMDRGSDGKLQEVGQVSVSLQRASGDFQTTKLNGFEVFARFGSAIAPLGDLDQDGFN
  DIAIAAPYGGEDKKGIVYIFNGRSTGLNAVPSQILEGQWAARSMPPSFGYSMKGATDIDKNGYPDLIVGA
  FGVDRAILYRARPVITVNAGLEVYPSILNQDNKTCSLPGTALKVSCFNVRFCLKADGKGVLPRKLNFQVE
  LLLDKLKQKGAIRRALFLYSRSPSHSKNMTISRGGLMQCEELIAYLRDESEFRDKLTPITIFMEYRLDYR
  TAADTTGLQPILNQFTPANISRQAHILLDCGEDNVCKPKLEVSVDSDQKKIYIGDDNPLTLIVKAQNQGE
  GAYEAELIVSIPLQADFIGVVRNNEALARLSCAFKTENQTRQVVCDLGNPMKAGTQLLAGLRFSVHQQSE
  MDTSVKFDLQIQSSNLFDKVSPVVSHKVDLAVLAAVEIRGVSSPDHIFLPIPNWEHKENPETEEDVGPVV
  QHIYELRNNGPSSFSKAMLHLQWPYKYNNNTLLYILHYDIDGPMNCTSDMEINPLRIKISSLQTTEKNDT
  VAGQGERDHLITKRDLALSEGDIHTLGCGVAQCLKIVCQVGRLDRGKSAILYVKSLLWTETFMNKENQNH
  SYSLKSSASFNVIEFPYKNLPIEDITNSTLVTTNVTWGIQPAPMPVPVWVIILAVLAGLLLLAVLVFVMY
  RMGFFKRVRPPQEEQEREQLQPHENGEGNSET
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145000
• ORF Size: 3036 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145000.2
• RefSeq ORF: 3039 bp
• Locus ID: 3685
• UniProt ID: P06756
• Cytogenetics: 2q32.1
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cell adhesion molecules (CAMs), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Pathways in cancer, Regulation of actin cytoskeleton, Small cell lung cancer
• MW: 112.1 kDa
• Gene Summary: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha V subunit. This subunit associates with beta 1, beta 3, beta 5, beta 6 and beta 8 subunits. The heterodimer consisting of alpha V and beta 3 subunits is also known as the vitronectin receptor. This integrin may regulate angiogenesis and cancer progression. Alternative splicing results in multiple transcript variants. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes. [provided by RefSeq, Oct 2015]