DBPA (YBX3) (NM_001145426) Human Tagged ORF Clone

CAT#: RC227614

CSDA (Myc-DDK-tagged)-Human cold shock domain protein A (CSDA), transcript variant 2

Product Images

Circular map for RC227614

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DBPA
• Synonyms: CSDA; CSDA1; DBPA; ZONAB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227614 representing NM_001145426
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGAGGCGGGCGAGGCCACCACCACCACCACCACCACCCTCCCGCAGGCTCCGACGGAGGCGGCCG
  CCGCGGCTCCCCAGGACCCCGCGCCCAAGAGCCCGGTGGGCAGCGGTGCGCCCCAGGCCGCGGCCCCGGC
  GCCCGCCGCCCACGTCGCAGGAAACCCCGGTGGGGACGCGGCCCCCGCAGCCACGGGCACCGCGGCCGCC
  GCCTCTTTAGCCGCCGCCGCCGGCAGCGAAGACGCGGAGAAAAAAGTTCTCGCCACCAAAGTCCTTGGCA
  CTGTCAAATGGTTCAACGTCAGAAATGGATATGGATTTATAAATCGAAATGACACCAAAGAAGATGTATT
  TGTACATCAGACTGCCATCAAGAAGAATAACCCACGGAAATATCTGCGCAGTCTAGGAGATGGAGAAACT
  GTAGAGTTTGATGTGGTTGAAGGAGAGAAGGGTGCAGAAGCTGCCAATGTGACTGGCCCGGATGGAGTTC
  CTGTGGAAGGGAGTCGTTACGCTGCAGATCGGCGCCGTTACAGACGTGGCTACTATGGAAGGCGCCGTGG
  CCCTCCCCGGAATGCTGGTGAGATTGGAGAGATGAAGGATGGAGTCCCAGAGGGAGCACAACTTCAGGGA
  CCGGTTCATCGAAATCCAACTTACCGCCCAAGGTACCGTAGCAGGGGACCTCCTCGCCCACGACCTGCCC
  CAGCAGTTGGAGAGGCTGAAGATAAAGAAAATCAGCAAGCCACCAGTGGTCCAAACCAGCCGTCTGTTCG
  CCGTGGATATCGGCGTCCCTACAATTACCGGCGTCGCCCGCGTCCTCCTAACGCTCCTTCACAAGATGGC
  AAAGAGGCCAAGGCAGGTGAAGCACCAACTGAGAACCCTGCTCCACCCACCCAGCAGAGCAGTGCTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227614 representing NM_001145426
  Red=Cloning site Green=Tags(s)
  
  MSEAGEATTTTTTTLPQAPTEAAAAAPQDPAPKSPVGSGAPQAAAPAPAAHVAGNPGGDAAPAATGTAAA
  ASLAAAAGSEDAEKKVLATKVLGTVKWFNVRNGYGFINRNDTKEDVFVHQTAIKKNNPRKYLRSLGDGET
  VEFDVVEGEKGAEAANVTGPDGVPVEGSRYAADRRRYRRGYYGRRRGPPRNAGEIGEMKDGVPEGAQLQG
  PVHRNPTYRPRYRSRGPPRPRPAPAVGEAEDKENQQATSGPNQPSVRRGYRRPYNYRRRPRPPNAPSQDG
  KEAKAGEAPTENPAPPTQQSSAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145426
• ORF Size: 909 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145426.1, NP_001138898.1
• RefSeq ORF: 912 bp
• Locus ID: 8531
• UniProt ID: P16989
• Cytogenetics: 12p13.2
• Protein Families: Transcription Factors
• Protein Pathways: Tight junction
• MW: 31.8 kDa
• Gene Summary: Binds to the GM-CSF promoter. Seems to act as a repressor. Binds also to full-length mRNA and to short RNA sequences containing the consensus site 5'-UCCAUCA-3'. May have a role in translation repression (By similarity).[UniProtKB/Swiss-Prot Function]