L1CAM (NM_001143963) Human Tagged ORF Clone

CAT#: RC227350

L1CAM (Myc-DDK-tagged)-Human L1 cell adhesion molecule (L1CAM), transcript variant 3

Product Images

Circular map for RC227350

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1CAM
• Synonyms: CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227350 representing NM_001143963
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCGTGGCGCTGCGGTACGTGTGGCCTCTCCTCCTCTGCAGCCCCTGCCTGCTTATCCAGATCCCCG
  AGGAATTGATGGAGCCACCTGTCATCACGGAACAGTCTCCACGGCGCCTGGTTGTCTTCCCCACAGATGA
  CATCAGCCTCAAGTGTGAGGCCAGTGGCAAGCCCGAAGTGCAGTTCCGCTGGACGAGGGATGGTGTCCAC
  TTCAAACCCAAGGAAGAGCTGGGTGTGACCGTGTACCAGTCGCCCCACTCTGGCTCCTTCACCATCACGG
  GCAACAACAGCAACTTTGCTCAGAGGTTCCAGGGCATCTACCGCTGCTTTGCCAGCAATAAGCTGGGCAC
  CGCCATGTCCCATGAGATCCGGCTCATGGCCGAGGGTGCCCCCAAGTGGCCAAAGGAGACAGTGAAGCCC
  GTGGAGGTGGAGGAAGGGGAGTCAGTGGTTCTGCCTTGCAACCCTCCCCCAAGTGCAGAGCCTCTCCGGA
  TCTACTGGATGAACAGCAAGATCTTGCACATCAAGCAGGACGAGCGGGTGACGATGGGCCAGAACGGCAA
  CCTCTACTTTGCCAATGTGCTCACCTCCGACAACCACTCAGACTACATCTGCCACGCCCACTTCCCAGGC
  ACCAGGACCATCATTCAGAAGGAACCCATTGACCTCCGGGTCAAGGCCACCAACAGCATGATTGACAGGA
  AGCCGCGCCTGCTCTTCCCCACCAACTCCAGCAGCCACCTGGTGGCCTTGCAGGGGCAGCCATTGGTCCT
  GGAGTGCATCGCCGAGGGCTTTCCCACGCCCACCATCAAATGGCTGCGCCCCAGTGGCCCCATGCCAGCC
  GACCGTGTCACCTACCAGAACCACAACAAGACCCTGCAGCTGCTGAAAGTGGGCGAGGAGGATGATGGCG
  AGTACCGCTGCCTGGCCGAGAACTCACTGGGCAGTGCCCGGCATGCGTACTATGTCACCGTGGAGGCTGC
  CCCGTACTGGCTGCACAAGCCCCAGAGCCATCTATATGGGCCAGGAGAGACTGCCCGCCTGGACTGCCAA
  GTCCAGGGCAGGCCCCAACCAGAGGTCACCTGGAGAATCAACGGGATCCCTGTGGAGGAGCTGGCCAAAG
  ACCAGAAGTACCGGATTCAGCGTGGCGCCCTGATCCTGAGCAACGTGCAGCCCAGTGACACAATGGTGAC
  CCAATGTGAGGCCCGCAACCGGCACGGGCTCTTGCTGGCCAATGCCTACATCTACGTTGTCCAGCTGCCA
  GCCAAGATCCTGACTGCGGACAATCAGACGTACATGGCTGTCCAGGGCAGCACTGCCTACCTTCTGTGCA
  AGGCCTTCGGAGCGCCTGTGCCCAGTGTTCAGTGGCTGGACGAGGATGGGACAACAGTGCTTCAGGACGA
  ACGCTTCTTCCCCTATGCCAATGGGACCCTGGGCATTCGAGACCTCCAGGCCAATGACACCGGACGCTAC
  TTCTGCCTGGCTGCCAATGACCAAAACAATGTTACCATCATGGCTAACCTGAAGGTTAAAGATGCAACTC
  AGATCACTCAGGGGCCCCGCAGCACAATCGAGAAGAAAGGTTCCAGGGTGACCTTCACGTGCCAGGCCTC
  CTTTGACCCCTCCTTGCAGCCCAGCATCACCTGGCGTGGGGACGGTCGAGACCTCCAGGAGCTTGGGGAC
  AGTGACAAGTACTTCATAGAGGATGGGCGCCTGGTCATCCACAGCCTGGACTACAGCGACCAGGGCAACT
  ACAGCTGCGTGGCCAGTACCGAACTGGATGTGGTGGAGAGTAGGGCACAGCTCTTGGTGGTGGGGAGCCC
  TGGGCCGGTGCCACGGCTGGTGCTGTCCGACCTGCACCTGCTGACGCAGAGCCAGGTGCGCGTGTCCTGG
  AGTCCTGCAGAAGACCACAATGCCCCCATTGAGAAATATGACATTGAATTTGAGGACAAGGAAATGGCGC
  CTGAAAAATGGTACAGTCTGGGCAAGGTTCCAGGGAACCAGACCTCTACCACCCTCAAGCTGTCGCCCTA
  TGTCCACTACACCTTTAGGGTTACTGCCATAAACAAATATGGCCCCGGGGAGCCCAGCCCGGTCTCTGAG
  ACTGTGGTCACACCTGAGGCAGCCCCAGAGAAGAACCCTGTGGATGTGAAGGGGGAAGGAAATGAGACCA
  CCAATATGGTCATCACGTGGAAGCCGCTCCGGTGGATGGACTGGAACGCCCCCCAGGTTCAGTACCGCGT
  GCAGTGGCGCCCTCAGGGGACACGAGGGCCCTGGCAGGAGCAGATTGTCAGCGACCCCTTCCTGGTGGTG
  TCCAACACGTCCACCTTCGTGCCCTATGAGATCAAAGTCCAGGCCGTCAACAGCCAGGGCAAGGGACCAG
  AGCCCCAGGTCACTATCGGCTACTCTGGAGAGGACTACCCCCAGGCAATCCCTGAGCTGGAAGGCATTGA
  AATCCTCAACTCAAGTGCCGTGCTGGTCAAGTGGCGGCCGGTGGACCTGGCCCAGGTCAAGGGCCACCTC
  CGCGGATACAATGTGACGTACTGGAGGGAGGGCAGTCAGAGGAAGCACAGCAAGAGACATATCCACAAAG
  ACCATGTGGTGGTGCCCGCCAACACCACCAGTGTCATCCTCAGTGGCTTGCGGCCCTATAGCTCCTACCA
  CCTGGAGGTGCAGGCCTTTAACGGGCGAGGATCGGGGCCCGCCAGCGAGTTCACCTTCAGCACCCCAGAG
  GGAGTGCCTGGCCACCCCGAGGCGTTGCACCTGGAGTGCCAGTCGAACACCAGCCTGCTGCTGCGCTGGC
  AGCCCCCACTCAGCCACAACGGCGTGCTCACCGGCTACGTGCTCTCCTACCACCCCCTGGATGAGGGGGG
  CAAGGGGCAACTGTCCTTCAACCTTCGGGACCCCGAACTTCGGACACACAACCTGACCGATCTCAGCCCC
  CACCTGCGGTACCGCTTCCAGCTTCAGGCCACCACCAAAGAGGGCCCTGGTGAAGCCATCGTACGGGAAG
  GAGGCACTATGGCCTTGTCTGGGATCTCAGATTTTGGCAACATCTCAGCCACAGCGGGTGAAAACTACAG
  TGTCGTCTCCTGGGTCCCCAAGGAGGGCCAGTGCAACTTCAGGTTCCATATCTTGTTCAAAGCCTTGGGA
  GAAGAGAAGGGTGGGGCTTCCCTTTCGCCACAGTATGTCAGCTACAACCAGAGCTCCTACACGCAGTGGG
  ACCTGCAGCCTGACACTGACTACGAGATCCACTTGTTTAAGGAGAGGATGTTCCGGCACCAAATGGCTGT
  GAAGACCAATGGCACAGGCCGCGTGAGGCTCCCTCCTGCTGGCTTCGCCACTGAGGGCTGGTTCATCGGC
  TTTGTGAGTGCCATCATCCTCCTGCTCCTCGTCCTGCTCATCCTCTGCTTCATCAAGCGCAGCAAGGGCG
  GCAAATACTCAGTGAAGGATAAGGAGGACACCCAGGTGGACTCTGAGGCCCGACCGATGAAAGATGAGAC
  CTTCGGCGAGTACAGTGACAACGAGGAGAAGGCCTTTGGCAGCAGCCAGCCATCGCTCAACGGGGACATC
  AAGCCCCTGGGCAGTGACGACAGCCTGGCCGATTATGGGGGCAGCGTGGATGTTCAGTTCAACGAGGATG
  GTTCGTTCATTGGCCAGTACAGTGGCAAGAAGGAGAAGGAGGCGGCAGGGGGCAATGACAGCTCAGGGGC
  CACTTCCCCCATCAACCCTGCCGTGGCCCTAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227350 representing NM_001143963
  Red=Cloning site Green=Tags(s)
  
  MVVALRYVWPLLLCSPCLLIQIPEELMEPPVITEQSPRRLVVFPTDDISLKCEASGKPEVQFRWTRDGVH
  FKPKEELGVTVYQSPHSGSFTITGNNSNFAQRFQGIYRCFASNKLGTAMSHEIRLMAEGAPKWPKETVKP
  VEVEEGESVVLPCNPPPSAEPLRIYWMNSKILHIKQDERVTMGQNGNLYFANVLTSDNHSDYICHAHFPG
  TRTIIQKEPIDLRVKATNSMIDRKPRLLFPTNSSSHLVALQGQPLVLECIAEGFPTPTIKWLRPSGPMPA
  DRVTYQNHNKTLQLLKVGEEDDGEYRCLAENSLGSARHAYYVTVEAAPYWLHKPQSHLYGPGETARLDCQ
  VQGRPQPEVTWRINGIPVEELAKDQKYRIQRGALILSNVQPSDTMVTQCEARNRHGLLLANAYIYVVQLP
  AKILTADNQTYMAVQGSTAYLLCKAFGAPVPSVQWLDEDGTTVLQDERFFPYANGTLGIRDLQANDTGRY
  FCLAANDQNNVTIMANLKVKDATQITQGPRSTIEKKGSRVTFTCQASFDPSLQPSITWRGDGRDLQELGD
  SDKYFIEDGRLVIHSLDYSDQGNYSCVASTELDVVESRAQLLVVGSPGPVPRLVLSDLHLLTQSQVRVSW
  SPAEDHNAPIEKYDIEFEDKEMAPEKWYSLGKVPGNQTSTTLKLSPYVHYTFRVTAINKYGPGEPSPVSE
  TVVTPEAAPEKNPVDVKGEGNETTNMVITWKPLRWMDWNAPQVQYRVQWRPQGTRGPWQEQIVSDPFLVV
  SNTSTFVPYEIKVQAVNSQGKGPEPQVTIGYSGEDYPQAIPELEGIEILNSSAVLVKWRPVDLAQVKGHL
  RGYNVTYWREGSQRKHSKRHIHKDHVVVPANTTSVILSGLRPYSSYHLEVQAFNGRGSGPASEFTFSTPE
  GVPGHPEALHLECQSNTSLLLRWQPPLSHNGVLTGYVLSYHPLDEGGKGQLSFNLRDPELRTHNLTDLSP
  HLRYRFQLQATTKEGPGEAIVREGGTMALSGISDFGNISATAGENYSVVSWVPKEGQCNFRFHILFKALG
  EEKGGASLSPQYVSYNQSSYTQWDLQPDTDYEIHLFKERMFRHQMAVKTNGTGRVRLPPAGFATEGWFIG
  FVSAIILLLLVLLILCFIKRSKGGKYSVKDKEDTQVDSEARPMKDETFGEYSDNEEKAFGSSQPSLNGDI
  KPLGSDDSLADYGGSVDVQFNEDGSFIGQYSGKKEKEAAGGNDSSGATSPINPAVALE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001143963
• ORF Size: 3744 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001143963.2
• RefSeq ORF: 3747 bp
• Locus ID: 3897
• UniProt ID: P32004
• Cytogenetics: Xq28
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Axon guidance, Cell adhesion molecules (CAMs)
• MW: 138.91 kDa
• Gene Summary: The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]