KCTD1 (NM_001142730) Human Tagged ORF Clone

CAT#: RC227186

KCTD1 (Myc-DDK-tagged)-Human potassium channel tetramerisation domain containing 1 (KCTD1), transcript variant 3

Product Images

Circular map for RC227186

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCTD1
• Synonyms: C18orf5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227186 representing NM_001142730
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGAATGCCTGGCAGCGGGGACTGTAACACCAGCGCGGGCGGCAGCGCCAGCGCTGCCGCCGCCG
  CCGCCGAGAACAATGGGGAGCGGGGCGAGGGCGAGCGCGGCGCGGGGGGCCGCGGCCGCCGCCACAGCCG
  TCCGCACTACTGCAGCGCGGGCGAGGAGGAGGAGGAAGAGGAGGAGGAGGACGAGATCCAGGAGGTGCAG
  ATAACGGGGGACGAGGAGGAGGAGGAGGACGGAGGTGGGGGGCTGGAGGAGGACGAGGAGGAGGAGGAAG
  AGGAGGAGATGGGGCTGGACTGGGACGAGCCCCTGGAGCCCGAGGACTCGGCCGGGGAGGAGCTGGAGCC
  CGAGCCGGTCCATATGATCAATATGGACCAGAGCGCCGCGCTGGAGCCCGAGGCGCCGCCGCGACTGCTG
  GCGCCCCGGGCCCGCGGTGGGCCGCCCGGGGACGGCTCCGAGCTGGACCCCGACGTGCTGCAGCGCCCCG
  AGCGGGCCCGGCTGAGCGAGAACACCCGGCTGGCCACCCGCTACGCCGTGCGCATCTTCCGGGAGTACCT
  GAGCGAGAAGGCGCAGAGCCCGGACTTCGAGACCATGGACAAGGGGGCGCTGTGCCGCGTGCTGCGCTCC
  TTCTATGCCGAGGCCCGCTCCAAAAGCGGCCAGCTCTACAGCAAGTCGTCGCTCATCAGCATCCGCAGCT
  CCCTCAACCGCTACCTCAATGAGCCCCCGTACTGCCGCACGCTCGACCTCACCAAGGACCCCGAGCTGCG
  CAGCGCCAACCTGACGCTGGCCGCGGTCATCCGCAAGCTCGAGGAGCAGGGCGCCGGGCCGGTGGTGCAG
  AAGCAAGCCATCACGCGCGCCGACCTGCGCAAGCTGTACACCTCCAGCGTCTTCAGCACCAACACGCCCT
  TCGGGCTGCTCAACAAGGTCTGGTTCGAGACGTGCATGTACTTCTGTACCCGCGGCCGCGAGAACCAGCG
  TGAGTTGGAGGAGGACTCTTTTGGGCTGGCCATGGACGAGGACGGTCGCAAGTTCGTCTACTTCAAGTCC
  CTCGGGCCCTACCACAAGTCGCGCTCGTCGTCGTGGAGCAAGAAGCGCGCCGAGAGCAGCGACGAGGAGA
  ACTTGCCCCGCATGTATGAGACGGGCACCGAGTTCTGCCCCTACGCCAGCTTCGTCAAGTACCTGTCGAA
  ACGCAACCCTCTCTGCAAGGCGTTCTTCCAGCGGCCCCGGGACCACTGCAGCGAGGGCGATGTGACCTGG
  TACGAGAACAAAGCCATCGGCAAGAACTTGCTAGGCACTCGGATGCAGATGCTCTCCAAGGCGGCCAAGC
  TCTCCAAGACCTACACCAACCACTGCATCGGCGCCGTCTCCATCGCCACGCTCAACAGCATCGCGGGCAT
  TGGCACCAAGCTGGGCTCGCCCGCCCCGCAGGGCTGCTACGCCGAGGCTCTGAACGGGGCGGCACGGCAC
  CACTCCCACCACCCCCCCACCCATCCCTCCCACCACCACCGCCCCCAGCCGCCCTCGCTGGGGAACACTT
  ACATCCTCCCCAAAGACAGCCAGGTCGGGCCCGACGTGAAATCCGAGGCTGCGCCCAAGCGCGCCCTGTA
  CGAGTCTGTGTTCGGGTCGGGGGAAATCTGCGGCCCCACTTCCCCCAAAAGACTTTGTATCCGCCCCTCG
  GAGCCTGTGGATGCGGTGGTGGTGGTTTCCGTGAAACACGACCCCCTGCCTCTTCTTCCAGAAGCCAATG
  GGCACAGAAGCACCAATTCTCCCACAATAGTTTCACCTGCTATTGTTTCCCCCACCCAGGACAGTCGGCC
  CAATATGTCAAGACCTCTGATCACTAGATCCCCTGCATCTCCACTGAACAACCAAGGCATCCCTACTCCA
  GCACAACTCACAAAATCCAATGCGCCTGTCCACATTGATGTGGGCGGCCACATGTACACCAGCAGCCTGG
  CCACCCTCACCAAATACCCTGAATCCAGAATCGGAAGACTTTTTGATGGTACAGAGCCCATTGTTTTGGA
  CAGTCTCAAACAGCACTATTTCATTGACAGAGATGGACAGATGTTCAGATATATCTTGAATTTTCTACGA
  ACATCCAAACTCCTCATTCCTGATGATTTCAAGGACTACACTTTGTTATATGAAGAGGCAAAATATTTTC
  AGCTTCAGCCCATGTTGTTGGAGATGGAAAGATGGAAGCAGGACAGAGAAACTGGTCGATTTTCAAGGCC
  CTGTGAGTGCCTCGTCGTGCGTGTGGCCCCAGACCTCGGAGAAAGGATCACGCTAAGCGGTGACAAATCC
  TTGATAGAAGAAGTATTTCCAGAGATCGGCGACGTGATGTGTAACTCTGTCAATGCAGGCTGGAATCACG
  ACTCGACGCACGTCATCAGGTTTCCACTAAATGGCTACTGTCACCTCAACTCAGTCCAGGTCCTCGAGAG
  GTTGCAGCAAAGAGGATTTGAAATCGTGGGCTCCTGTGGGGGAGGAGTAGACTCGTCCCAGTTCAGCGAA
  TACGTCCTTCGGCGGGAACTGAGGCGGACGCCCCGTGTACCCTCCGTCATCCGGATAAAGCAAGAGCCTC
  TGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227186 representing NM_001142730
  Red=Cloning site Green=Tags(s)
  
  MARMPGSGDCNTSAGGSASAAAAAAENNGERGEGERGAGGRGRRHSRPHYCSAGEEEEEEEEEDEIQEVQ
  ITGDEEEEEDGGGGLEEDEEEEEEEEMGLDWDEPLEPEDSAGEELEPEPVHMINMDQSAALEPEAPPRLL
  APRARGGPPGDGSELDPDVLQRPERARLSENTRLATRYAVRIFREYLSEKAQSPDFETMDKGALCRVLRS
  FYAEARSKSGQLYSKSSLISIRSSLNRYLNEPPYCRTLDLTKDPELRSANLTLAAVIRKLEEQGAGPVVQ
  KQAITRADLRKLYTSSVFSTNTPFGLLNKVWFETCMYFCTRGRENQRELEEDSFGLAMDEDGRKFVYFKS
  LGPYHKSRSSSWSKKRAESSDEENLPRMYETGTEFCPYASFVKYLSKRNPLCKAFFQRPRDHCSEGDVTW
  YENKAIGKNLLGTRMQMLSKAAKLSKTYTNHCIGAVSIATLNSIAGIGTKLGSPAPQGCYAEALNGAARH
  HSHHPPTHPSHHHRPQPPSLGNTYILPKDSQVGPDVKSEAAPKRALYESVFGSGEICGPTSPKRLCIRPS
  EPVDAVVVVSVKHDPLPLLPEANGHRSTNSPTIVSPAIVSPTQDSRPNMSRPLITRSPASPLNNQGIPTP
  AQLTKSNAPVHIDVGGHMYTSSLATLTKYPESRIGRLFDGTEPIVLDSLKQHYFIDRDGQMFRYILNFLR
  TSKLLIPDDFKDYTLLYEEAKYFQLQPMLLEMERWKQDRETGRFSRPCECLVVRVAPDLGERITLSGDKS
  LIEEVFPEIGDVMCNSVNAGWNHDSTHVIRFPLNGYCHLNSVQVLERLQQRGFEIVGSCGGGVDSSQFSE
  YVLRRELRRTPRVPSVIRIKQEPLD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142730
• ORF Size: 2595 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142730.3
• RefSeq Size: 3438 bp
• RefSeq ORF: 2598 bp
• Locus ID: 284252
• UniProt ID: Q719H9
• Cytogenetics: 18q11.2
• Protein Families: Ion Channels: Other
• MW: 96.4 kDa
• Gene Summary: This gene encodes a protein containing a BTB (Broad-complex, tramtrack and bric a brac), also known as a POZ (POxvirus and zinc finger) protein-protein interaction domain. The encoded protein negatively regulates the AP-2 family of transcription factors and the Wnt signaling pathway. A mechanism for the modulation of Wnt signaling has been proposed in which the encoded protein enhances ubiquitination and degradation of the beta-catenin protein. Mutations in this gene have been identified in Scalp-ear-nipple (SEN) syndrome. [provided by RefSeq, May 2017]