SPART (NM_001142294) Human Tagged ORF Clone

CAT#: RC227155

SPG20 (Myc-DDK-tagged)-Human spastic paraplegia 20 (Troyer syndrome) (SPG20), transcript variant 4

Product Images

Circular map for RC227155

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  Western blot validation of overexpression lysate (Cat# LY428019) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227169 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SPART protein (Cat# TP327155). The protein was produced from HEK293T cells transfected with SPART cDNA clone (Cat# RC227155) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPART
• Synonyms: SPG20; TAHCCP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227155 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAAGAGCCACAAAATGGAGAACCTGCTGAAATTAAGATCATCAGAGAAGCATATAAGAAGGCCT
  TTTTATTTGTTAACAAAGGTCTGAATACAGATGAATTAGGTCAGAAGGAAGAAGCAAAGAACTACTATAA
  GCAAGGAATAGGACACCTGCTCAGAGGGATCAGCATTTCATCAAAAGAGTCTGAACACACAGGTACTGGG
  TGGGAATCTGCTAGACAGATGCAACAGAAAATGAAAGAAACTCTACAGAATGTACGCACCAGGCTGGAAA
  TTCTAGAGAAGGGTCTTGCCACTTCTCTGCAGAATGATCTTCAGGAGGTGCCCAAGTTATATCCAGAATT
  TCCACCTAAAGACATGTGTGAAAAATTACCAGAGCCTCAGTCTTTTAGTTCAGCTCCTCAGCATGCTGAA
  GTAAATGGAAACACCTCAACTCCAAGTGCAGGGGCAGTTGCTGCACCTGCTTCTCTGTCTTTACCATCAC
  AAAGTTGTCCAGCAGAAGCTCCTCCTGCTTATACTCCTCAAGCTGCTGAAGGTCACTACACTGTATCCTA
  TGGAACAGATTCTGGGGAGTTTTCATCAGTTGGAGAGGAGTTTTATAGGAATCATTCTCAGCCACCGCCT
  CTTGAGACCTTAGGGCTGGATGCAGATGAATTGATTTTGATACCAAATGGAGTACAGATTTTTTTTGTAA
  ATCCTGCAGGGGAGGTTAGTGCACCTTCGTATCCTGGGTACCTTCGAATTGTGAGGTTTTTGGATAATTC
  TCTCGATACGGTTCTAAACCGTCCTCCCGGGTTTCTTCAGGTTTGTGACTGGTTATATCCTCTAGTTCCT
  GATAGATCTCCGGTTCTGAAATGTACTGCGGGAGCCTACATGTTTCCTGATACAATGCTACAAGCAGCAG
  GATGCTTTGTGGGGGTCGTCCTGTCCTCTGAGTTACCAGAGGATGATAGAGAGCTCTTTGAGGATCTGTT
  AAGGCAAATGTCTGACCTTCGGCTCCAGGCCAACTGGAACAGAGCAGAAGAAGAAAATGAATTCCAAATC
  CCTGGAAGAACTAGACCCTCCTCTGACCAACTAAAAGAAGCCTCTGGCACTGATGTGAAACAGTTGGACC
  AAGGCAATAAGGATGTACGTCATAAAGGAAAACGTGGAAAAAGGGCTAAAGATACTTCAAGTGAAGAAGT
  TAACCTGAGTCACATTGTACCATGTGAGCCAGTTCCAGAAGAAAAGCCAAAAGAATTACATGAATGGAGT
  GAAAAAGTGGCTCACAACATTTTGTCAGGTGCTTCCTGGGTGAGTTGGGGTTTAGTCAAAGGTGCTGAGA
  TTACTGGTAAGGCAATCCAGAAAGGTGCTTCTAAACTCCGAGAGCGGATTCAACCAGAAGAAAAACCCGT
  GGAAGTTAGTCCAGCTGTCACCAAGGGACTTTATATAGCGAAGCAAGCTACAGGAGGAGCAGCAAAAGTC
  AGTCAGTTCCTGGTTGATGGAGTTTGCACTGTAGCAAATTGCGTTGGAAAAGAACTAGCTCCACATGTCA
  AGAAGCATGGAAGCAAACTTGTTCCAGAATCTCTTAAAAAAGACAAAGATGGGAAATCTCCTCTGGATGG
  TGCTATGGTTGTAGCAGCGAGTAGTGTTCAAGGATTTTCAACTGTCTGGCAAGGATTGGAATGTGCAGCT
  AAATGCATCGTTAACAATGTTTCAGCAGAAACTGTACAAACTGTCAGATACAAATACGGATATAATGCAG
  GAGAAGCTACCCACCATGCGGTGGATTCTGCGGTCAATGTTGGCGTAACTGCCTACAATATTAACAACAT
  TGGTATCAAAGCAATGGTGAAGAAAACTGCAACACAAACAGGACACACTCTCCTTGAGGACTATCAGATA
  GTTGATAATTCTCAGAGGGAAAATCAAGAAGGAGCAGCAAATGTCAACGTGAGAGGGGAGAAGGATGAGC
  AGACGAAGGAAGTAAAGGAGGCAAAGAAGAAAGATAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227155 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEQEPQNGEPAEIKIIREAYKKAFLFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISISSKESEHTGTG
  WESARQMQQKMKETLQNVRTRLEILEKGLATSLQNDLQEVPKLYPEFPPKDMCEKLPEPQSFSSAPQHAE
  VNGNTSTPSAGAVAAPASLSLPSQSCPAEAPPAYTPQAAEGHYTVSYGTDSGEFSSVGEEFYRNHSQPPP
  LETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVP
  DRSPVLKCTAGAYMFPDTMLQAAGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQANWNRAEEENEFQI
  PGRTRPSSDQLKEASGTDVKQLDQGNKDVRHKGKRGKRAKDTSSEEVNLSHIVPCEPVPEEKPKELHEWS
  EKVAHNILSGASWVSWGLVKGAEITGKAIQKGASKLRERIQPEEKPVEVSPAVTKGLYIAKQATGGAAKV
  SQFLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKKDKDGKSPLDGAMVVAASSVQGFSTVWQGLECAA
  KCIVNNVSAETVQTVRYKYGYNAGEATHHAVDSAVNVGVTAYNINNIGIKAMVKKTATQTGHTLLEDYQI
  VDNSQRENQEGAANVNVRGEKDEQTKEVKEAKKKDK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142294
• ORF Size: 1998 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142294.1, NP_001135766.1
• RefSeq Size: 4838 bp
• RefSeq ORF: 2001 bp
• Locus ID: 23111
• UniProt ID: Q8N0X7
• Cytogenetics: 13q13.3
• MW: 72.9 kDa
• Gene Summary: This gene encodes a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain, and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with microtubules. Stimulation with epidermal growth factor (EGF) results in protein translocation to the plasma membrane, and the protein functions in the degradation and intracellular trafficking of EGF receptor. Multiple alternatively spliced variants, encoding the same protein, have been identified. Mutations associated with this gene cause autosomal recessive spastic paraplegia 20 (Troyer syndrome). [provided by RefSeq, Nov 2008]