FAM178A (SLF2) (NM_001136123) Human Tagged ORF Clone

CAT#: RC227146

FAM178A (Myc-DDK-tagged)-Human family with sequence similarity 178, member A (FAM178A), transcript variant 2

Product Images

Circular map for RC227146

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAM178A
• Synonyms: C10orf6; FAM178A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227146 representing NM_001136123
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAGGCGCTGCATGCCCGCTAGGCCAGGTTTCCCCTCATCCCCAGCCCCGGGGTCGTCGCCCCCGC
  GCTGCCATCTGAGACCCGGTAGTACCGCCCATGCTGCAGCGGGAAAGAGAACAGAGAGTCCTGGGGACAG
  GAAGCAGTCAATTATAGATTTCTTCAAACCAGCTTCAAAACAAGACAGACACATGTTGGATTCACCACAA
  AAATCAAACATCAAATATGGAGGAAGTAGATTGTCTATCACTGGGACAGAGCAGTTTGAAAGGAAACTAT
  CCTCACCAAAAGAATCTAAACCCAAAAGGGTGCCACCAGAAAAGAGCCCTATTATAGAAGCTTTCATGAA
  AGGTGTTAAAGAGCACCATGAAGATCATGGTATACATGAGTCACGTCGGCCTTGTCTGTCACTAGCCTCC
  AAATATTTAGCCAAAGGAACAAATATCTATGTTCCTTCTTCATATCACTTGCCAAAGGAGATGAAGTCAC
  TAAAGAAAAAACATCGATCCCCAGAGAGAAGGAAGTCACTATTCATTCATGAAAATAATGAGAAGAATGA
  TAGAGATCGAGGCAAAACCAATGCAGACTCCAAAAAGCAGACCACAGTGGCAGAAGCTGACATCTTCAAT
  AACAGCTCCAGAAGCCTTAGCAGCAGGAGCAGCCTGTCCAGGCACCACCCGGAAGAAAGCCCACTGGGAG
  CTAAATTCCAGTTGTCACTAGCTTCTTACTGCAGAGAACGAGAACTAAAGAGGTTGAGAAAGGAGCAAAT
  GGAGCAGAGAATCAACTCCGAGAATTCTTTCTCAGAAGCAAGCAGTCTTTCCTTAAAATCTAGTATAGAA
  AGAAAATATAAACCAAGGCAGGAACAAAGGAAACAGAATGACATCATACCTGGAAAAAATAATCTGTCAA
  ATGTGGAAAATGGACATCTCTCAAGAAAAAGATCCTCTTCTGATTCATGGGAACCTACTTCAGCAGGCTC
  TAAGCAGAATAAATTCCCTGAAAAAAGAAAAAGGAACTCTGTGGACTCAGATCTGAAAAGCACAAGAGAA
  TCTATGATACCAAAAGCAAGAGAGTCCTTCCTTGAGAAGCGTCCTGATGGACCACATCAGAAAGAAAAAT
  TTATAAAACATATTGCACTGAAGACACCTGGTGATGTGTTGCGCTTAGAAGATATATCCAAGGAACCGAG
  TGATGAAACTGATGGCTCTTCTGCAGGCTTGGCACCTTCAAATTCTGGCAATTCTGGCCACCATTCTACC
  AGGAATAGTGACCAAATCCAAGTGGCAGGTACCAAGGAGACTAAGATGCAGAAACCCCACTTACCTTTAT
  CTCAGGAAAAGTCTGCAATTAAAAAAGCTAGCAACCTTCAGAAAAATAAAACCGCTAGCTCCACGACAAA
  GGAGAAGGAGACAAAACTACCTTTACTTTCCCGTGTTCCAAGTGCTGGTTCCTCTCTAGTACCATTAAAT
  GCTAAAAATTGTGCTCTTCCAGTTTCTAAAAAAGATAAAGAGCGTTCCTCATCTAAAGAATGTTCTGGGC
  ATTCTACAGAATCCACCAAACACAAGGAACACAAAGCAAAGACTAATAAGGCCGATTCTAATGTATCTTC
  AGGGAAAATTTCTGGGGGACCTTTGCGCTCAGAATATGGCACTCCTACAAAGTCTCCCCCTGCTGCTTTG
  GAAGTTGTGCCATGTATCCCAAGCCCTGCAGCACCTTCAGATAAAGCCCCTTCAGAAGGAGAGAGTTCAG
  GAAATTCCAATGCAGGTAGCAGTGCACTGAAAAGAAAACTAAGGGGTGATTTTGATAGTGATGAAGAAAG
  TTTAGGTTACAACCTAGACAGTGATGAGGAAGAGGAAACATTAAAGTCACTGGAAGAAATAATGGCTTTG
  AACTTCAATCAGACTCCTGCAGCTACAGGAAAGCCTCCTGCTCTTTCCAAGGGGCTTAGATCTCAGTCAT
  CAGACTATACAGGACATGTTCATCCTGGAACTTACACAAATACCTTAGAACGTCTAGTGAAGGAAATGGA
  AGACACACAAAGGCTAGATGAACTGCAGAAGCAACTACAAGAAGACATAAGGCAAGGCCGAGGCATTAAA
  TCCCCAATCAGAATTGGAGAAGAAGACAGTACAGATGATGAGGATGGCCTCTTAGAAGAGCACAAGGAAT
  TTCTAAAGAAATTTTCAGTTACAATTGATGCTATTCCTGATCATCATCCAGGTGAAGAAATATTTAATTT
  CCTCAATTCTGGAAAAATTTTCAATCAGTATACCTTGGATTTAAGAGACTCTGGTTTTATTGGACAAAGT
  GCTGTAGAAAAACTTATTCTTAAATCGGGAAAAACAGATCAGATTTTTTTGACAACACAAGGTTTCCTTA
  CGTCTGCTTATCACTATGTCCAGTGTCCTGTCCCTGTGTTAAAGTGGCTGTTTCGGATGATGTCAGTTCA
  TACAGACTGTATTGTGTCAGTGCAGATTTTAAGTACATTGATGGAAATAACAATTAGAAATGATACCTTC
  AGTGACTCACCAGTTTGGCCATGGATCCCATCATTGTCTGATGTAGCAGCTGTGTTTTTCAATATGGGGA
  TTGATTTTAGATCTTTGTTTCCCCTGGAGAATCTTCAGCCAGACTTTAATGAAGACTATCTAGTTTCTGA
  AACACAGACAACATCAAGGGGGAAAGAAAGTGAAGATTCATCTTATAAGCCAATTTTTTCAACACTTCCT
  GAAACCAACATTTTAAATGTGGTTAAGTTTCTAGGCTTGTGTACATCTATACATCCAGAAGGTTACCAGG
  ATCGTGAAATAATGTTGCTGATTTTAATGTTATTTAAAATGAGTTTGGAAAAACAGCTGAAACAGATTCC
  TTTAGTAGACTTTCAAAGCCTCCTGATAAACCTGATGAAAAACATCAGAGATTGGAACACAAAGGTGCCT
  GAACTCTGTCTGGGCATAAATGAACTCTCCAGTCATCCCCACAACCTCCTGTGGTTGGTACAGCTGGTCC
  CTAATTGGACATCACGTGGAAGGCAACTGAGACAGTGCCTCAGTCTAGTGATTATTTCAAAGCTTTTGGA
  TGAGAAACACGAAGATGTTCCTAATGCCAGTAATCTGCAGGTATCAGTCCTACATCGCTATCTTGTGCAG
  ATGAAGCCTTCTGATTTGTTAAAGAAAATGGTCTTGAAGAAAAAGGCTGAACAACCAGATGGCATTATTG
  ATGACAGTCTTCATTTAGAACTTGAAAAGCAGGCATATTACCTGACCTACATTCTTCTTCATTTAGTCGG
  TGAAGTTAGTTGTTCTCATTCTTTTTCTTCTGGACAACGGAAACACTTTGTGCTACTCTGTGGGGCTTTG
  GAAAAGCATGTTAAATGTGATATTAGGGAAGATGCAAGACTTTTTTACAGAACTAAGGTGAAAGACTTGG
  TCGCCAGGATACATGGAAAATGGCAGGAAATAATCCAGAACTGTCGGCCTACTCAGGTGTCATTTTGTTA
  TACAATTTCATGTATTCTTAATAGTTTTGCTGAATGGCACTCTTCATACTGTTTGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227146 representing NM_001136123
  Red=Cloning site Green=Tags(s)
  
  MTRRCMPARPGFPSSPAPGSSPPRCHLRPGSTAHAAAGKRTESPGDRKQSIIDFFKPASKQDRHMLDSPQ
  KSNIKYGGSRLSITGTEQFERKLSSPKESKPKRVPPEKSPIIEAFMKGVKEHHEDHGIHESRRPCLSLAS
  KYLAKGTNIYVPSSYHLPKEMKSLKKKHRSPERRKSLFIHENNEKNDRDRGKTNADSKKQTTVAEADIFN
  NSSRSLSSRSSLSRHHPEESPLGAKFQLSLASYCRERELKRLRKEQMEQRINSENSFSEASSLSLKSSIE
  RKYKPRQEQRKQNDIIPGKNNLSNVENGHLSRKRSSSDSWEPTSAGSKQNKFPEKRKRNSVDSDLKSTRE
  SMIPKARESFLEKRPDGPHQKEKFIKHIALKTPGDVLRLEDISKEPSDETDGSSAGLAPSNSGNSGHHST
  RNSDQIQVAGTKETKMQKPHLPLSQEKSAIKKASNLQKNKTASSTTKEKETKLPLLSRVPSAGSSLVPLN
  AKNCALPVSKKDKERSSSKECSGHSTESTKHKEHKAKTNKADSNVSSGKISGGPLRSEYGTPTKSPPAAL
  EVVPCIPSPAAPSDKAPSEGESSGNSNAGSSALKRKLRGDFDSDEESLGYNLDSDEEEETLKSLEEIMAL
  NFNQTPAATGKPPALSKGLRSQSSDYTGHVHPGTYTNTLERLVKEMEDTQRLDELQKQLQEDIRQGRGIK
  SPIRIGEEDSTDDEDGLLEEHKEFLKKFSVTIDAIPDHHPGEEIFNFLNSGKIFNQYTLDLRDSGFIGQS
  AVEKLILKSGKTDQIFLTTQGFLTSAYHYVQCPVPVLKWLFRMMSVHTDCIVSVQILSTLMEITIRNDTF
  SDSPVWPWIPSLSDVAAVFFNMGIDFRSLFPLENLQPDFNEDYLVSETQTTSRGKESEDSSYKPIFSTLP
  ETNILNVVKFLGLCTSIHPEGYQDREIMLLILMLFKMSLEKQLKQIPLVDFQSLLINLMKNIRDWNTKVP
  ELCLGINELSSHPHNLLWLVQLVPNWTSRGRQLRQCLSLVIISKLLDEKHEDVPNASNLQVSVLHRYLVQ
  MKPSDLLKKMVLKKKAEQPDGIIDDSLHLELEKQAYYLTYILLHLVGEVSCSHSFSSGQRKHFVLLCGAL
  EKHVKCDIREDARLFYRTKVKDLVARIHGKWQEIIQNCRPTQVSFCYTISCILNSFAEWHSSYCLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136123
• ORF Size: 3558 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136123.2
• RefSeq ORF: 3561 bp
• Locus ID: 55719
• UniProt ID: Q8IX21
• Cytogenetics: 10q24.31
• MW: 133.2 kDa
• Gene Summary: Plays a role in the DNA damage response (DDR) pathway by regulating postreplication repair of UV-damaged DNA and genomic stability maintenance (PubMed:25931565). The SLF1-SLF2 complex acts to link RAD18 with the SMC5-SMC6 complex at replication-coupled interstrand cross-links (ICL) and DNA double-strand breaks (DSBs) sites on chromatin during DNA repair in response to stalled replication forks (PubMed:25931565). Promotes the recruitment of the SMC5-SMC6 complex to DNA lesions (PubMed:25931565).[UniProtKB/Swiss-Prot Function]