NFKBIL1 (NM_001144962) Human Tagged ORF Clone

CAT#: RC226786

NFKBIL1 (Myc-DDK-tagged)-Human nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1 (NFKBIL1), transcript variant 3

Product Images

Circular map for RC226786

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NFKBIL1
• Synonyms: IKBL; NFKBIL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226786 representing NM_001144962
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCACTTCCCGCCGCCAACGCCGAGAACGTCGCTTTCGTCGTTACTTGTCTGCAGGACGGCTGG
  TCCGGGCCCAGGCCCTCCTCCAGCGACACCCAGGCCTCGATGTAGATGCTGGGCAGCCCCCACCACTGCA
  CCGGGCCTGTGCCCGCCACGATGCCCCTGCCCTGTGCCTGCTGCTTCGGCTCGGGGCTGACCCTGCCCAC
  CAGGACCGCCATGGGGACACGGCACTGCATGCTGCTGCCCGCCAGGGCCCAGATGCCTACACCGATTTCT
  TCCTCCCGCTGCTAAGCCGCTGTCCCTCCGCCATGGGAATAAAGAATAAGGATGGGGAGACCCCTGGCCA
  AATTTTGGGCTGGGGACCCCCCTGGGATTCTGCTGAAGAGGAGGAAGAAGATGATGCCTCCAAGGAGCGG
  GAATGGAGACAGAAGCTCCAGGGTGAGCTGGAGGACGAGTGGCAGGAAGTCATGGGGAGGTTTGAAGGTG
  ATGCCTCCCATGAAACCCAGGAACCTGAGTCCTTCTCAGCCTGGTCAGATCGCCTGGCCCGGGAACATGC
  CCAGAAGTGCCAGCAGCAGCAGCGAGAAGCAGAGGGATCCCGTCGACCCCCACGTGCTGAGGGCTCCAGC
  CAGAGCTGGCGACAGCAGGAGGAGGAGCAGCGGCTCTTCAGGGAGCGAGCCCGGGCCAAGGAGGAAGAGC
  TGCGTGAGAGCCGAGCCAGGAGGGCGCAGGAGGCTCTAGGGGACCGAGAACCCAAGCCAACCAGGGCCGG
  GCCCAGGGAAGAGCACCCCAGAGGAGCGGGGAGGGGCAGCCTCTGGCGATTTGGTGATGTGCCCTGGCCC
  TGCCCTGGGGGAGGGGACCCAGAGGCCATGGCTGCAGCCCTGGTGGCCAGGGGCCCCCCTTTGGAGGAAC
  AGGGGGCTCTGAGGAGGTACTTGAGGGTCCAGCAGGTCCGCTGGCACCCTGACCGCTTCCTGCAGCGATT
  CCGAAGCCAGATTGAGACCTGGGAGCTGGGCCGTGTGATGGGAGCAGTGACAGCCCTTTCTCAGGCCCTG
  AATCGCCATGCAGAGGCCCTCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226786 representing NM_001144962
  Red=Cloning site Green=Tags(s)
  
  MASTSRRQRRERRFRRYLSAGRLVRAQALLQRHPGLDVDAGQPPPLHRACARHDAPALCLLLRLGADPAH
  QDRHGDTALHAAARQGPDAYTDFFLPLLSRCPSAMGIKNKDGETPGQILGWGPPWDSAEEEEEDDASKER
  EWRQKLQGELEDEWQEVMGRFEGDASHETQEPESFSAWSDRLAREHAQKCQQQQREAEGSRRPPRAEGSS
  QSWRQQEEEQRLFRERARAKEEELRESRARRAQEALGDREPKPTRAGPREEHPRGAGRGSLWRFGDVPWP
  CPGGGDPEAMAAALVARGPPLEEQGALRRYLRVQQVRWHPDRFLQRFRSQIETWELGRVMGAVTALSQAL
  NRHAEALK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001144962
• ORF Size: 1074 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001144962.2
• RefSeq Size: 1457 bp
• RefSeq ORF: 1077 bp
• Locus ID: 4795
• UniProt ID: Q9UBC1
• Cytogenetics: 6p21.33
• Protein Families: Transcription Factors
• MW: 40.8 kDa
• Gene Summary: This gene encodes a divergent member of the I-kappa-B family of proteins. Its function has not been determined. The gene lies within the major histocompatibility complex (MHC) class I region on chromosome 6. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]