RCOR3 (NM_001136224) Human Tagged ORF Clone

CAT#: RC226775

RCOR3 (Myc-DDK-tagged)-Human REST corepressor 3 (RCOR3), transcript variant 2

Product Images

Circular map for RC226775

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RCOR3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226775 representing NM_001136224
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGGCATGATGGAGAAAGGGCCCGAGTTACTGGGGAAGAACCGATCGGCCAACGGCAGCGCCAAGA
  GCCCGGCAGGCGGCGGCGGCAGCGGCGCCTCGTCCACCAACGGCGGGCTGCACTACTCAGAGCCCGAGAG
  CGGCTGCAGCAGCGACGACGAGCACGATGTTGGGATGAGAGTCGGAGCCGAATACCAAGCTCGGATCCCT
  GAATTTGATCCAGGTGCTACAAAGTACACAGATAAAGACAATGGAGGGATGCTTGTATGGTCTCCATATC
  ACAGTATCCCAGATGCCAAATTGGATGAATACATTGCAATTGCAAAGGAAAAGCATGGCTACAATGTGGA
  ACAGGCACTTGGCATGTTGTTCTGGCATAAACATAACATTGAGAAGTCCCTTGCTGATCTCCCTAATTTC
  ACTCCCTTTCCGGATGAGTGGACAGTGGAAGATAAAGTCCTATTTGAACAAGCCTTTAGTTTTCATGGAA
  AGAGCTTTCACAGGATTCAGCAAATGCTTCCAGATAAGACAATTGCAAGCCTTGTAAAATATTACTATTC
  TTGGAAAAAAACTCGCTCTAGGACAAGTTTGATGGATCGCCAGGCTCGTAAACTAGCTAATAGACATAAT
  CAGGGTGACAGTGATGATGATGTAGAAGAAACACATCCAATGGATGGGAATGATAGTGATTATGATCCCA
  AAAAAGAAGCCAAAAAAGAGGGTAATACTGAACAACCTGTCCAAACTAGCAAGATTGGACTTGGAAGAAG
  AGAGTATCAGAGTTTACAACATCGCCATCATTCTCAGCGTTCTAAGTGCCGTCCACCTAAGGGCATGTAT
  TTAACCCAGGAAGATGTGGTAGCAGTTTCCTGTAGTCCCAATGCAGCCAACACCATCCTGAGGCAACTGG
  ACATGGAGTTGATCTCTCTAAAACGTCAGGTTCAGAATGCTAAGCAAGTAAACAGTGCACTTAAACAGAA
  AATGGAAGGTGGAATTGAAGAATTCAAACCTCCTGAGTCAAATCAGAAAATTAATGCCCGTTGGACCACA
  GAGGAGCAGCTTCTAGCAGTGCAAGGCACAGACCCCACAGGCTCCTCGGACACTGGGTCCATCACCTCCT
  GCCCCATCATCCACTCCAACACCAACAGCCCCTATTGCCACTCTGAACCAGCCTCCACCACTTCTTCGTC
  CAACACTGCCTGCTGCCCCGGCTCTTCACCGGCAGCCTCCTCCACTCCAGCAGCAGGCTCGGTTCATCCA
  GCCCCGGCCAACTTTAAATCAGCCTCCACCACCTCTTATTCGCCCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226775 representing NM_001136224
  Red=Cloning site Green=Tags(s)
  
  MPGMMEKGPELLGKNRSANGSAKSPAGGGGSGASSTNGGLHYSEPESGCSSDDEHDVGMRVGAEYQARIP
  EFDPGATKYTDKDNGGMLVWSPYHSIPDAKLDEYIAIAKEKHGYNVEQALGMLFWHKHNIEKSLADLPNF
  TPFPDEWTVEDKVLFEQAFSFHGKSFHRIQQMLPDKTIASLVKYYYSWKKTRSRTSLMDRQARKLANRHN
  QGDSDDDVEETHPMDGNDSDYDPKKEAKKEGNTEQPVQTSKIGLGRREYQSLQHRHHSQRSKCRPPKGMY
  LTQEDVVAVSCSPNAANTILRQLDMELISLKRQVQNAKQVNSALKQKMEGGIEEFKPPESNQKINARWTT
  EEQLLAVQGTDPTGSSDTGSITSCPIIHSNTNSPYCHSEPASTTSSSNTACCPGSSPAASSTPAAGSVHP
  APANFKSASTTSYSPC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136224
• ORF Size: 1308 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136224.3
• RefSeq Size: 4232 bp
• RefSeq ORF: 1311 bp
• Locus ID: 55758
• UniProt ID: Q9P2K3
• Cytogenetics: 1q32.2-q32.3
• Protein Families: Transcription Factors
• MW: 47.8 kDa
• Gene Summary: May act as a component of a corepressor complex that represses transcription.[UniProtKB/Swiss-Prot Function]