NCOA4 (NM_001145261) Human Tagged ORF Clone

CAT#: RC226676

NCOA4 (Myc-DDK-tagged)-Human nuclear receptor coactivator 4 (NCOA4), transcript variant 2

Product Images

Circular map for RC226676

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NCOA4
• Synonyms: ARA70; ELE1; PTC3; RFG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226676 representing NM_001145261
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGCCCAGTTGACCACTTTTGCTCTAGCTGGAGCAGTGAGGAGAATGAATACCTTCCAAGACCAGA
  GTGGCAGCTCCAGTAATAGAGAACCCCTTTTGAGGTGTAGTGATGCACGGAGGGACTTGGAGCTTGCTAT
  TGGTGGAGTTCTCCGGGCTGAACAGCAAATTAAAGATAACTTGCGAGAGGTCAAAGCTCAGATTCACAGT
  TGCATAAGCCGTCACCTGGAATGTCTTAGAAGCCGTGAGGTATGGCTGTATGAACAGGTGGACCTTATTT
  ATCAGCTTAAAGAGGAGACACTTCAACAGCAGGCTCAGCAGCTCTACTCGTTATTGGGCCAGTTCAATTG
  TCTTACTCATCAACTGGAGTGTACCCAAAACAAAGATCTAGCCAATCAAGTCTCTGTGTGCCTGGAGAGA
  CTGGGCAGTTTGACCCTTAAGCCTGAAGATTCAACTGTCCTGCTCTTTGAAGCTGACACAATTACTCTGC
  GCCAGACCATCACCACATTTGGGTCTCTCAAAACCATTCAAATTCCTGAGCACTTGATGGCTCATGCTAG
  TTCAGCAAATATTGGGCCCTTCCTGGAGAAGAGAGGCTGTATCTCCATGCCAGAGCAGAAGTCAGCATCC
  GGTATTGTAGCTGTCCCTTTCAGCGAATGGCTCCTTGGAAGCAAACCTGCCAGTGGTTATCAAGCTCCTT
  ACATACCCAGCACCGACCCCCAGGACTGGCTTACCCAAAAGCAGACCTTGGAGAACAGTCAGACTTCTTC
  CAGAGCCTGCAATTTCTTCAATAATGTCGGGGGAAACCTAAAGGGCTTAGAAAACTGGCTCCTCAAGAGT
  GAAAAATCAAGTTATCAAAAGTGTAACAGCCATTCCACTACTAGTTCTTTCTCCATTGAAATGGAAAAGG
  TTGGAGATCAAGAGCTTCCTGATCAAGATGAGATGGACCTATCAGATTGGCTAGTGACTCCCCAGGAATC
  CCATAAGCTGCGGAAGCCTGAGAATGGCAGTCGTGAAACCAGTGAGAAGTTTAAGCTCTTATTCCAGTCC
  TATAATGTGAATGATTGGCTTGTCAAGACTGACTCCTGTACCAACTGTCAGGGAAACCAGCCCAAAGGTG
  TGGAGATTGAAAACCTGGGCAATCTGAAGTGCCTGAATGACCACTTGGAGGCCAAGAAACCATTGTCCAC
  CCCCAGCATGGTTACAGAGGATTGGCTTGTCCAGAACCATCAGGACCCATGTAAGGTAGAGGAGGTGTGC
  AGAGCCAATGAGCCCTGCACAAGCTTTGCAGAGTGTGTGTGTGATGAGAATTGTGAGAAGGAGGCTCTGT
  ATAAGTGGCTTCTGAAGAAAGAAGGAAAGGATAAAAATGGGATGCCTGTGGAACCCAAACCTGAGCCTGA
  GAAGCATAAAGATTCCCTGAATATGTGGCTCTGTCCTAGAAAAGAAGTAATAGAACAAACTAAAGCACCA
  AAGGCAATGACTCCTTCTAGAATTGCTGATTCCTTCCAAGTCATAAAGAACAGCCCCTTGTCGGAGTGGC
  TTATCAGGCCCCCATACAAAGAAGGAAGTCCCAAGGAAGTGCCTGGTACTGAAGACAGAGCTGGCAAACA
  GAAGTTTAAAAGCCCCATGAATACTTCCTGGTGTTCCTTTAACACAGCTGACTGGGTCCTGCCAGGAAAG
  AAGATGGGCAACCTCAGCCAGTTATCTTCTGGAGAAGACAAGTGGCTGCTTCGAAAGAAGGCCCAGGAAG
  TATTACTTAATTCACCTCTACAGGAGGAACATAACTTCCCCCCAGACCATTATGGCCTCCCTGCAGTTTG
  TGATCTCTTTGCCTGTATGCAGCTTAAAGTTGATAAAGAGAAGTGGTTATATCGAACTCCTCTACAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226676 representing NM_001145261
  Red=Cloning site Green=Tags(s)
  
  MGAQLTTFALAGAVRRMNTFQDQSGSSSNREPLLRCSDARRDLELAIGGVLRAEQQIKDNLREVKAQIHS
  CISRHLECLRSREVWLYEQVDLIYQLKEETLQQQAQQLYSLLGQFNCLTHQLECTQNKDLANQVSVCLER
  LGSLTLKPEDSTVLLFEADTITLRQTITTFGSLKTIQIPEHLMAHASSANIGPFLEKRGCISMPEQKSAS
  GIVAVPFSEWLLGSKPASGYQAPYIPSTDPQDWLTQKQTLENSQTSSRACNFFNNVGGNLKGLENWLLKS
  EKSSYQKCNSHSTTSSFSIEMEKVGDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLFQS
  YNVNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDHLEAKKPLSTPSMVTEDWLVQNHQDPCKVEEVC
  RANEPCTSFAECVCDENCEKEALYKWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLCPRKEVIEQTKAP
  KAMTPSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTEDRAGKQKFKSPMNTSWCSFNTADWVLPGK
  KMGNLSQLSSGEDKWLLRKKAQEVLLNSPLQEEHNFPPDHYGLPAVCDLFACMQLKVDKEKWLYRTPLQM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145261
• ORF Size: 1890 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145261.1, NP_001138733.1
• RefSeq ORF: 1893 bp
• Locus ID: 8031
• UniProt ID: Q13772
• Cytogenetics: 10q11.22
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Thyroid cancer
• MW: 71.2 kDa
• Gene Summary: This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14. [provided by RefSeq, Feb 2009]