PATL2 (NM_001145112) Human Tagged ORF Clone

CAT#: RC226641

PATL2 (Myc-DDK-tagged)-Human protein associated with topoisomerase II homolog 2 (yeast) (PATL2)

Product Images

Circular map for RC226641

• 

  Western blot validation of overexpression lysate (Cat# LY428698) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226641 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PATL2
• Synonyms: hPat1a; OOMD4; Pat1a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226641 representing NM_001145112
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTGCCTTGAAGGGCCAGGTAAGACCTGTGGCCCCTTGGCTTCTGAGGAGGAGCTGGTGTCTGCCT
  GCCAGTTGGAAAAAGAAGAAGAGAATGAAGGGGAGGAGGAGGAAGAGGAGGAGGACGAGGAGGATCTGGA
  CCCAGATCTGGACCCAGACCTAGAGGAGGAAGAGAATGATCTTGGGGATCCAGCTGTACTTGGTGCTGTC
  CACAACACCCAGAGAGCTCTGCTTAGCTCCCCTGGAGTCAAGGCCCCTGGTATGCTGGGAATGTCACTTG
  CCTCCTTGCATTTTCTGTGGCAGACCTTGGACTACCTGTCGCCCATCCCTTTCTGGCCTACATTTCCCAG
  CACCAGCTCTCCAGCACAGCACTTTGGACCTCGGCTGCCCTCACCAGACCCAACTCTCTTCTGCAGCCTG
  CTGACCTCGTGGCCCCCTAGGTTCAGTCATCTGACCCAGCTCCACCCTCGGCACCAACGAATCTTGCAGC
  AGCAGCAGCATAGTCAAACACCAAGTCCCCCAGCCAAGAAGCCTTGGTCTCAGCAGCCAGACCCCTATGC
  TAACCTCATGACCAGAAAAGAGAAGGACTGGGTGATAAAAGTGCAGATGGTGCAGCTGCAGAGTGCAAAA
  CCCCGCCTGGATGACTACTATTACCAGGAATATTACCAGAAGCTAGAGAAGAAGCAGGCAGACGAAGAGC
  TACTTGGACGAAGAAACCGGGTTGAGTCCCTCAAGCTGGTAACGCCTTACATTCCGAAGGCAGAGGCTTA
  TGAGTCCGTGGTCCGAATCGAGGGTTCCCTGGGCCAGGTAGCTGTGTCGACATGCTTCAGCCCTCGCCGA
  GCTATTGATGCGGTACCCCATGGAACTCAAGAGCAGGATATAGAAGCTGCAAGCAGTCAGAGGCTTCGGG
  TATTATACCGGATTGAGAAGATGTTCCTTCAGTTACTAGAAATAGAGGAAGGCTGGAAGTATAGGCCTCC
  ACCGCCCTGCTTTTCTGAGCAGCAAAGCAACCAGGTTGAGAAGCTCTTCCAGACCTTAAAGACCCAGGAG
  CAGAACAACCTGGAAGAGGCAGCAGATGGCTTCCTGCAGGTGCTCTCTGTGAGGAAGGGGAAGGCCCTGG
  TGGCCCGGCTGCTCCCCTTCCTGCCCCAGGATCAGGCTGTTACCATTCTTTTGGCTATCACCCACCATCT
  GCCCCTCCTGGTCCGGAGGGATGTGGCTGATCAGGCCCTACAAATGTTATTCAAACCTCTGGGCAAATGT
  ATTAGTCACTTGACCCTCCACGAACTCCTCCAAGGACTTCAGGGATTAACGCTGTTGCCACCTGGCTCCT
  CAGAGCGGCCAGTCACCGTGGTGCTTCAGAATCAGTTTGGAATATCTTTGCTCTATGCCCTGCTGAGCCA
  TGGGGAGCAACTGGTATCGCTGCATTCTTCCCTAGAGGAACCCAACAGTGACCATACAGCTTGGACAGAC
  ATGGTGGTTCTGATTGCCTGGGAGATAGCCCAAATGCCTACAGCCTCTCTGGCAGAACCCCTAGCTTTCC
  CCAGCAACCTACTTCCCCTGTTCTGTCACCACGTGGACAAACAATTGGTTCAGCAGCTGGAGGCCAGGAT
  GGAGTTTGCCTGGATTTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226641 representing NM_001145112
  Red=Cloning site Green=Tags(s)
  
  MNCLEGPGKTCGPLASEEELVSACQLEKEEENEGEEEEEEEDEEDLDPDLDPDLEEEENDLGDPAVLGAV
  HNTQRALLSSPGVKAPGMLGMSLASLHFLWQTLDYLSPIPFWPTFPSTSSPAQHFGPRLPSPDPTLFCSL
  LTSWPPRFSHLTQLHPRHQRILQQQQHSQTPSPPAKKPWSQQPDPYANLMTRKEKDWVIKVQMVQLQSAK
  PRLDDYYYQEYYQKLEKKQADEELLGRRNRVESLKLVTPYIPKAEAYESVVRIEGSLGQVAVSTCFSPRR
  AIDAVPHGTQEQDIEAASSQRLRVLYRIEKMFLQLLEIEEGWKYRPPPPCFSEQQSNQVEKLFQTLKTQE
  QNNLEEAADGFLQVLSVRKGKALVARLLPFLPQDQAVTILLAITHHLPLLVRRDVADQALQMLFKPLGKC
  ISHLTLHELLQGLQGLTLLPPGSSERPVTVVLQNQFGISLLYALLSHGEQLVSLHSSLEEPNSDHTAWTD
  MVVLIAWEIAQMPTASLAEPLAFPSNLLPLFCHHVDKQLVQQLEARMEFAWIY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145112
• ORF Size: 1629 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145112.1, NP_001138584.1
• RefSeq ORF: 1632 bp
• Locus ID: 197135
• UniProt ID: C9JE40
• Cytogenetics: 15q21.1
• MW: 61.3 kDa
• Gene Summary: RNA-binding protein that acts as a translational repressor.[UniProtKB/Swiss-Prot Function]