Nuclear Factor 1 (NFIA) (NM_001145512) Human Tagged ORF Clone

CAT#: RC226505

NFIA (Myc-DDK-tagged)-Human nuclear factor I/A (NFIA), transcript variant 4

Product Images

Circular map for RC226505

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nuclear Factor 1
• Synonyms: BRMUTD; CTF; NF-I/A; NF1-A; NFI-A; NFI-L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226505 representing NM_001145512
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAATGTGCCGCCCGGCCTCCTCCTCGGTTCTCTACGTGCCCACGCGGTGGCCCGGGGGGTGCGGGG
  CAACCTGGCAAAGTTGCCCAAGTCCTCCACCGAGACGCACACGCATACCCCAGCGCCCGGCAGTTATGTA
  TTCTCCGCTCTGTCTCACCCAGGATGAATTTCATCCTTTCATCGAAGCACTTCTGCCCCACGTCCGAGCC
  TTTGCCTACACATGGTTCAACCTGCAGGCCCGAAAACGAAAATACTTCAAAAAACATGAAAAGCGTATGT
  CAAAAGAAGAAGAGAGAGCCGTGAAGGATGAATTGCTAAGTGAAAAACCAGAGGTCAAGCAGAAGTGGGC
  ATCTCGACTTCTGGCAAAGTTGCGGAAAGATATCCGACCCGAATATCGAGAGGATTTTGTTCTTACAGTT
  ACAGGGAAAAAACCTCCATGTTGTGTTCTTTCCAACCCAGACCAGAAAGGCAAGATGCGAAGAATTGACT
  GCCTCCGCCAGGCAGATAAAGTCTGGAGGTTGGACCTTGTTATGGTGATTTTGTTTAAAGGTATTCCGCT
  GGAAAGTACTGATGGCGAGCGCCTTGTAAAGTCCCCACAATGCTCTAATCCAGGGCTCTGTGTCCAACCC
  CATCACATAGGGGTTTCTGTTAAGGAACTCGATTTATATTTGGCATACTTTGTGCATGCAGCAGATTCAA
  GTCAATCTGAAAGTCCCAGCCAGCCAAGTGACGCTGACATTAAGGACCAGCCAGAAAATGGACATTTGGG
  CTTCCAGGACAGTTTTGTCACATCAGGTGTTTTTAGTGTCACTGAGCTAGTAAGAGTGTCACAGACACCA
  ATAGCTGCAGGAACTGGCCCAAATTTTTCTCTCTCAGATTTGGAAAGTTCTTCATACTACAGCATGAGTC
  CAGGAGCAATGAGGAGGTCTTTACCCAGCACATCCTCTACGAGCTCCACAAAGCGCCTCAAGTCTGTGGA
  GGATGAAATGGACAGTCCTGGTGAGGAGCCATTTTATACAGGCCAAGGGCGCTCCCCAGGAAGTGGCAGT
  CAGTCAAGTGGATGGCATGAAGTGGAGCCAGGAATGCCATCTCCAACCACACTGAAGAAGTCGGAGAAGT
  CTGGTTTCAGCAGCCCCTCCCCTTCACAGACCTCCTCCCTGGGAACGGCGTTCACACAGCATCACCGACC
  TGTCATTACAGGACCCAGAGCAAGTCCGCATGCAACACCATCGACTCTTCATTTCCCGACATCACCCATT
  ATCCAGCAGCCTGGGCCTTACTTCTCACACCCAGCCATCCGCTATCACCCTCAGGAGACGCTGAAAGAAT
  TTGTCCAACTTGTCTGCCCTGATGCTGGTCAGCAGGCTGGACAGGTGGGGTTCCTCAATCCCAATGGGAG
  CAGCCAAGGCAAGGTGCACAACCCATTCCTTCCCACCCCAATGTTGCCACCGCCACCGCCACCACCGATG
  GCCAGGCCTGTGCCTCTGCCGGTGCCAGACACAAAGCCTCCAACCACGTCAACAGAAGGAGGTGCAGCCT
  CCCCCACGTCACCAACCTACTCGACACCCAGCACCTCCCCCGCAAACCGATTCGTCAGTGTTGGACCACG
  GGATCCAAGCTTTGTAAATATCCCTCAACAGACACAGTCCTGGTACCTGGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226505 representing NM_001145512
  Red=Cloning site Green=Tags(s)
  
  MQMCRPASSSVLYVPTRWPGGCGATWQSCPSPPPRRTRIPQRPAVMYSPLCLTQDEFHPFIEALLPHVRA
  FAYTWFNLQARKRKYFKKHEKRMSKEEERAVKDELLSEKPEVKQKWASRLLAKLRKDIRPEYREDFVLTV
  TGKKPPCCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKSPQCSNPGLCVQP
  HHIGVSVKELDLYLAYFVHAADSSQSESPSQPSDADIKDQPENGHLGFQDSFVTSGVFSVTELVRVSQTP
  IAAGTGPNFSLSDLESSSYYSMSPGAMRRSLPSTSSTSSTKRLKSVEDEMDSPGEEPFYTGQGRSPGSGS
  QSSGWHEVEPGMPSPTTLKKSEKSGFSSPSPSQTSSLGTAFTQHHRPVITGPRASPHATPSTLHFPTSPI
  IQQPGPYFSHPAIRYHPQETLKEFVQLVCPDAGQQAGQVGFLNPNGSSQGKVHNPFLPTPMLPPPPPPPM
  ARPVPLPVPDTKPPTTSTEGGAASPTSPTYSTPSTSPANRFVSVGPRDPSFVNIPQQTQSWYLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145512
• ORF Size: 1662 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145512.2
• RefSeq ORF: 1665 bp
• Locus ID: 4774
• UniProt ID: Q12857
• Cytogenetics: 1p31.3
• Protein Families: Transcription Factors
• MW: 60.7 kDa
• Gene Summary: This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]