RPTP mu (PTPRM) (NM_001105244) Human Tagged ORF Clone

SKU RC226398

PTPRM (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, M (PTPRM), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: RPTP mu
• Synonyms: hR-PTPu; PTPRL1; R-PTP-MU; RPTPM; RPTPU
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226398 representing NM_001105244; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGGGGACTTGGGACTTGCCTGGCGACTTTGGCCGGACTTTTGCTAACTGCGGCGGGCGAGACGTTCT; CAGGTGGCTGCCTCTTTGATGAGCCGTATAGCACATGTGGATATAGTCAATCTGAAGGTGATGACTTCAA; TTGGGAGCAAGTGAACACCTTGACTAAACCGACTTCTGATCCATGGATGCCATCAGGTTCTTTCATGCTG; GTGAATGCCTCTGGGAGACCTGAGGGGCAGAGAGCCCACCTGCTCTTACCCCAACTTAAAGAAAATGACA; CCCACTGCATCGATTTTCACTATTTTGTGTCCAGCAAGAGTAATTCTCCTCCGGGGTTACTCAATGTCTA; CGTGAAGGTCAATAACGGGCCACTGGGGAATCCTATCTGGAATATATCTGGAGACCCAACACGTACATGG; AACAGGGCAGAACTGGCCATTAGTACTTTCTGGCCTAACTTTTATCAGGTGATTTTTGAAGTGATAACTT; CTGGACATCAAGGCTATCTCGCTATCGATGAGGTGAAGGTGTTAGGACATCCATGTACCAGGACTCCTCA; CTTCCTGCGGATTCAGAATGTGGAAGTTAATGCTGGCCAGTTTGCTACCTTCCAGTGCAGTGCCATCGGC; AGGACCGTGGCAGGAGACAGGCTCTGGTTACAGGGCATTGATGTGCGAGATGCTCCTCTGAAGGAAATCA; AGGTGACCAGCTCCCGACGCTTCATTGCTTCATTTAATGTTGTGAATACCACCAAACGAGATGCTGGAAA; GTACCGCTGCATGATTCGCACTGAAGGAGGTGTTGGAATATCAAACTATGCAGAGTTGGTAGTTAAAGAA; CCACCCGTTCCTATTGCCCCACCTCAGCTCGCCTCTGTAGGAGCCACCTACCTGTGGATACAGCTCAACG; CCAACTCCATCAATGGGGATGGGCCCATTGTGGCCCGAGAGGTGGAGTACTGCACGGCCAGTGGGAGCTG; GAATGACCGGCAGCCAGTCGATTCCACGAGCTATAAAATTGGACACCTTGACCCAGATACAGAATATGAG; ATTAGTGTGCTCCTGACCAGGCCAGGGGAGGGTGGCACTGGCTCTCCTGGTCCAGCTCTCAGGACAAGAA; CAAAGTGTGCTGATCCCATGCGAGGCCCAAGAAAACTAGAAGTAGTGGAGGTCAAATCTCGGCAAATCAC; TATCCGCTGGGAGCCATTTGGATATAATGTAACTCGTTGCCACAGTTATAATCTCACTGTCCACTACTGT; TACCAAGTTGGAGGACAAGAACAAGTGCGAGAAGAAGTAAGCTGGGATACAGAAAACTCACACCCTCAAC; ACACGATCACTAACCTGTCACCATACACCAATGTCAGTGTGAAACTGATCCTCATGAACCCAGAGGGCCG; GAAGGAAAGCCAAGAACTCATAGTGCAGACAGATGAAGACCTCCCAGGTGCTGTTCCCACTGAATCCATA; CAAGGAAGTACCTTTGAAGAGAAGATATTTCTTCAGTGGAGAGAACCAACTCAAACATATGGTGTAATCA; CTTTATATGAGATCACCTACAAAGCAGTCAGTTCCTTTGACCCAGAAATAGATTTATCCAATCAGAGTGG; AAGAGTTTCAAAGCTGGGAAATGAAACCCATTTTCTGTTTTTTGGACTGTATCCGGGGACCACATACTCC; TTTACCATCCGAGCTAGCACAGCTAAGGGTTTTGGGCCTCCAGCAACAAACCAGTTCACCACCAAAATAT; CAGCACCCTCTATGCCAGCTTATGAACTTGAGACACCTTTGAATCAAACTGACAATACCGTGACAGTCAT; GCTGAAACCTGCCCACAGCAGAGGAGCACCTGTCAGTGTCTATCAAATAGTTGTTGAGGAAGAACGTCCT; CGAAGAACTAAAAAGACGACAGAAATCTTAAAGTGCTACCCAGTGCCAATTCACTTCCAGAATGCTTCTC; TGCTGAACTCACAGTACTACTTTGCTGCAGAATTTCCTGCAGACAGCCTCCAAGCTGCGCAGCCTTTTAC; AATTGGTGATAATAAGACATATAATGGATACTGGAACACTCCCCTTCTCCCCTATAAAAGCTACAGAATT; TATTTCCAAGCTGCTAGTAGAGCCAATGGGGAAACCAAAATAGACTGTGTCCAAGTGGCCACAAAAGGAG; CTGCCACTCCGAAACCAGTCCCAGAACCCGAGAAACAGACAGACCATACAGTTAAAATTGCTGGAGTCAT; CGCGGGCATCTTGCTGTTCGTGATTATATTTCTTGGAGTTGTGTTGGTAATGAAGAAAAGGAAACTGGCC; AAGAAGCGGAAAGAGACCATGAGCAGCACCCGACAGGAGATGACTGTGATGGTGAACTCAATGGACAAGA; GCTATGCTGAGCAGGGCACAAACTGCGACGAGGCTTTCTCATTCATGGACACGCACAATCTGAATGGGAG; ATCTGTGTCTTCACCATCGTCCTTCACAATGAAAACAAATACACTGAGCACATCGGTGCCTAATTCCTAT; TACCCAGACCCATTTGTGCCAACTGCAATTTTAGTGCCAATAAATGATGAAACCCACACAATGGCCAGCG; ATACCAGCAGCCTGGTGCAGTCCCATACTTACAAGAAGCGAGAGCCGGCCGACGTGCCCTATCAGACTGG; GCAGCTCCACCCCGCCATCCGGGTGGCAGACCTCCTTCAGCACATCACACAGATGAAGTGTGCGGAGGGC; TACGGCTTCAAGGAGGAATACGAGAGCTTCTTTGAAGGGCAGTCTGCACCATGGGACTCGGCTAAGAAAG; ATGAGAACAGAATGAAGAACAGATACGGGAATATCATTGCATACGATCATTCCCGAGTGAGGCTGCAGAC; AATAGAAGGAGACACAAACTCAGACTATATCAATGGCAATTATATCGATGGTTATCATCGACCCAATCAT; TACATTGCTACCCAAGGGCCAATGCAGGAAACCATCTATGACTTCTGGAGGATGGTGTGGCACGAAAACA; CTGCAAGTATCATCATGGTGACCAATCTTGTGGAAGTGGGAAGGGTCAAATGCTGCAAATACTGGCCAGA; TGACACAGAGATATATAAAGACATTAAAGTTACCCTAATAGAAACAGAACTACTGGCAGAATATGTGATA; AGAACATTTGCTGTTGAAAAGAGAGGTGTGCATGAAATCCGAGAGATCAGACAGTTTCACTTCACTGGCT; GGCCGGATCATGGGGTCCCCTACCATGCCACCGGCCTGCTGGGATTCGTGCGGCAAGTCAAGTCCAAGAG; CCCGCCCAGTGCAGGCCCACTGGTGGTGCACTGCAGTGCTGGTGCAGGGAGGACTGGCTGTTTCATCGTC; ATTGATATCATGTTGGACATGGCCGAAAGGGAAGGGGTCGTAGACATCTACAACTGCGTCAGGGAGCTGC; GGTCACGGAGGGTGAACATGGTGCAAACAGAGGAGCAGTATGTGTTTATCCACGATGCGATCCTGGAAGC; CTGTCTTTGTGGGGACACCTCTGTGCCTGCTTCCCAAGTTAGGTCTCTGTATTATGACATGAACAAACTG; GATCCACAGACAAACTCAAGCCAGATTAAAGAGGAATTCCGGACGCTAAACATGGTGACACCAACGCTGC; GAGTAGAGGACTGCAGCATCGCACTGTTGCCCCGGAACCATGAGAAAAACCGGTGCATGGACATCCTGCC; CCCAGACCGCTGCCTGCCCTTCCTCATCACCATCGATGGGGAGAGCAGCAACTACATCAATGCTGCCCTC; ATGGACAGCTATAAACAGCCTTCAGCTTTTATAGTCACCCAGCATCCTTTGCCAAACACAGTGAAAGACT; TTTGGAGACTGGTCCTGGATTATCACTGCACATCCGTAGTTATGCTAAATGATGTGGATCCTGCCCAGTT; GTGTCCACAGTACTGGCCAGAAAACGGAGTACACAGACACGGCCCCATCCAGGTGGAATTTGTCTCTGCT; GACCTGGAAGAGGACATCATCAGCAGGATATTCCGCATTTACAATGCCGCCAGACCCCAAGATGGATATC; GGATGGTGCAGCAATTCCAGTTCCTGGGCTGGCCGATGTACAGGGACACACCAGTGTCTAAGCGCTCCTT; CTTGAAGCTCATTCGCCAGGTGGACAAGTGGCAAGAGGAGTACAATGGCGGGGAAGGCCGCACGGTTGTG; CACTGCTTGAACGGGGGAGGCCGCAGTGGGACGTTCTGCGCCATCAGCATCGTATGTGAGATGCTCCGGC; ACCAGAGAACCGTGGATGTCTTTCACGCTGTGAAGACACTGAGGAACAACAAGCCCAACATGGTCGACCT; CCTGGATCAGTACAAGTTCTGCTACGAGGTGGCCCTGGAATACTTGAATTCTGGC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226398 representing NM_001105244; Red=Cloning site Green=Tags(s); ; MRGLGTCLATLAGLLLTAAGETFSGGCLFDEPYSTCGYSQSEGDDFNWEQVNTLTKPTSDPWMPSGSFML; VNASGRPEGQRAHLLLPQLKENDTHCIDFHYFVSSKSNSPPGLLNVYVKVNNGPLGNPIWNISGDPTRTW; NRAELAISTFWPNFYQVIFEVITSGHQGYLAIDEVKVLGHPCTRTPHFLRIQNVEVNAGQFATFQCSAIG; RTVAGDRLWLQGIDVRDAPLKEIKVTSSRRFIASFNVVNTTKRDAGKYRCMIRTEGGVGISNYAELVVKE; PPVPIAPPQLASVGATYLWIQLNANSINGDGPIVAREVEYCTASGSWNDRQPVDSTSYKIGHLDPDTEYE; ISVLLTRPGEGGTGSPGPALRTRTKCADPMRGPRKLEVVEVKSRQITIRWEPFGYNVTRCHSYNLTVHYC; YQVGGQEQVREEVSWDTENSHPQHTITNLSPYTNVSVKLILMNPEGRKESQELIVQTDEDLPGAVPTESI; QGSTFEEKIFLQWREPTQTYGVITLYEITYKAVSSFDPEIDLSNQSGRVSKLGNETHFLFFGLYPGTTYS; FTIRASTAKGFGPPATNQFTTKISAPSMPAYELETPLNQTDNTVTVMLKPAHSRGAPVSVYQIVVEEERP; RRTKKTTEILKCYPVPIHFQNASLLNSQYYFAAEFPADSLQAAQPFTIGDNKTYNGYWNTPLLPYKSYRI; YFQAASRANGETKIDCVQVATKGAATPKPVPEPEKQTDHTVKIAGVIAGILLFVIIFLGVVLVMKKRKLA; KKRKETMSSTRQEMTVMVNSMDKSYAEQGTNCDEAFSFMDTHNLNGRSVSSPSSFTMKTNTLSTSVPNSY; YPDPFVPTAILVPINDETHTMASDTSSLVQSHTYKKREPADVPYQTGQLHPAIRVADLLQHITQMKCAEG; YGFKEEYESFFEGQSAPWDSAKKDENRMKNRYGNIIAYDHSRVRLQTIEGDTNSDYINGNYIDGYHRPNH; YIATQGPMQETIYDFWRMVWHENTASIIMVTNLVEVGRVKCCKYWPDDTEIYKDIKVTLIETELLAEYVI; RTFAVEKRGVHEIREIRQFHFTGWPDHGVPYHATGLLGFVRQVKSKSPPSAGPLVVHCSAGAGRTGCFIV; IDIMLDMAEREGVVDIYNCVRELRSRRVNMVQTEEQYVFIHDAILEACLCGDTSVPASQVRSLYYDMNKL; DPQTNSSQIKEEFRTLNMVTPTLRVEDCSIALLPRNHEKNRCMDILPPDRCLPFLITIDGESSNYINAAL; MDSYKQPSAFIVTQHPLPNTVKDFWRLVLDYHCTSVVMLNDVDPAQLCPQYWPENGVHRHGPIQVEFVSA; DLEEDIISRIFRIYNAARPQDGYRMVQQFQFLGWPMYRDTPVSKRSFLKLIRQVDKWQEEYNGGEGRTVV; HCLNGGGRSGTFCAISIVCEMLRHQRTVDVFHAVKTLRNNKPNMVDLLDQYKFCYEVALEYLNSG; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001105244
• ORF Size: 4395 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001105244.2
• RefSeq ORF: 4398 bp
• Locus ID: 5797
• UniProt ID: P28827
• Cytogenetics: 18p11.23
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Protein Pathways: Adherens junction, Cell adhesion molecules (CAMs)
• MW: 165.06 kDa
• Summary: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains a meprin-A5 antigen-PTP mu (MAM) domain, an Ig-like domain and four fibronectin type III-like repeats. This PTP has been shown to mediate cell-cell aggregation through the interaction with another molecule of this PTP on an adjacent cell. This PTP can interact with scaffolding protein RACK1/GNB2L1, which may be necessary for the downstream signaling in response to cell-cell adhesion. Alternative splicing results in multiple transcripts encoding distinct isoforms. [provided by RefSeq, Jul 2008]