Angiomotin (AMOT) (NM_001113490) Human Tagged ORF Clone

CAT#: RC226319

AMOT (Myc-DDK-tagged)-Human angiomotin (AMOT), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AMOT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226319 representing NM_001113490
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAAATTCTGAAGAACAGCCAAGTGGAGGGACCACGGTATTGCAGCGTTTGCTACAAGAGCAGCTTC
  GCTATGGCAATCCTAGTGAGAATCGCAGCCTGCTTGCCATACACCAGCAAGCCACAGGGAATGGCCCTCC
  TTTCCCCAGTGGCAGTGGGAACCCGGGCCCTCAGAGTGATGTGTTGAGTCCCCAAGACCACCACCAACAG
  CTTGTGGCTCATGCTGCTCGACAAGAACCCCAGGGGCAGGAAATCCAGTCAGAAAACCTCATCATGGAGA
  AGCAGCTGTCTCCTCGAATGCAAAATAATGAAGAACTCCCGACCTATGAAGAAGCCAAGGTCCAGTCCCA
  GTACTTTCGGGGCCAACAGCATGCCAGTGTTGGAGCTGCCTTCTATGTCACTGGAGTCACCAACCAGAAG
  ATGAGGACTGAGGGACGCCCATCAGTTCAGCGGCTCAATCCTGGAAAGATGCACCAAGATGAGGGACTCA
  GAGACCTTAAGCAAGGGCATGTCCGTTCCTTGAGTGAACGACTAATGCAGATGTCACTGGCCACCAGTGG
  AGTTAAGGCCCATCCACCTGTTACCAGTGCTCCCCTCTCCCCACCACAACCCAATGACCTCTACAAGAAT
  CCCACAAGTTCCAGTGAATTCTACAAGGCCCAAGGGCCACTTCCTAACCAGCATAGCCTGAAGGGCATGG
  AACACCGAGGCCCCCCACCAGAATATCCCTTCAAGGGCATGCCACCCCAATCTGTAGTGTGCAAGCCCCA
  AGAGCCAGGGCACTTCTATAGTGAGCATCGCCTGAACCAGCCAGGGAGAACAGAGGGGCAACTGATGAGG
  TATCAGCATCCCCCTGAGTATGGAGCAGCCAGGCCAGCGCAGGACATCTCATTGCCATTGTCAGCCAGGA
  ACTCGCAGCCTCACAGCCCTACTTCTTCTCTGACCTCTGGGGGGTCCTTGCCCTTGCTACAATCTCCACC
  ATCCACTAGATTGTCTCCTGCCCGACACCCCTTGGTCCCAAACCAGGGAGACCATTCAGCTCACCTGCCT
  AGGCCGCAGCAGCATTTCCTTCCTAATCAGGCTCACCAGGGGGATCATTACCGTCTCTCCCAACCTGGCC
  TGAGTCAGCAGCAGCAGCAACAGCAGCAGCAGCACCATCATCACCATCACCACCAACAACAGCAGCAGCA
  GCAGCCACAGCAGCAGCCAGGAGAAGCCTATTCAGCTATGCCTCGGGCTCAGCCATCCTCTGCTTCTTAT
  CAGCCAGTGCCAGCAGACCCTTTTGCCATTGTTTCCAGAGCCCAGCAGATGGTTGAGATCCTCTCAGACG
  AGAACCGGAACTTGAGGCAAGAGTTGGAAGGATGCTATGAGAAGGTGGCAAGACTGCAGAAGGTGGAGAC
  AGAAATCCAGCGCGTCTCGGAGGCATATGAGAACCTCGTGAAGTCATCCTCCAAAAGAGAGGCCCTAGAG
  AAAGCCATGAGAAACAAGCTAGAGGGCGAGATTCGGAGGATGCATGATTTCAACAGGGATCTGAGAGAGC
  GTCTAGAGACTGCCAACAAGCAGCTTGCAGAGAAGGAATATGAGGGGTCAGAGGACACCAGAAAAACCAT
  CTCGCAGCTCTTTGCAAAAAATAAAGAAAGCCAGCGTGAGAAGGAGAAGCTGGAAGCGGAGCTGGCCACT
  GCCCGTTCTACCAATGAGGACCAAAGACGACACATCGAAATCCGAGATCAGGCCCTGAGTAATGCCCAGG
  CCAAGGTGGTAAAGCTGGAAGAAGAGCTGAAAAAGAAGCAAGTGTACGTTGACAAGGTGGAGAAGATGCA
  GCAGGCCCTTGTACAGCTCCAGGCAGCATGTGAAAAACGTGAGCAGCTAGAGCACCGTCTCCGGACACGA
  CTGGAGAGGGAACTGGAATCCCTGAGAATCCAGCAGCGTCAGGGCAACTGTCAGCCCACCAACGTTTCAG
  AATACAATGCTGCTGCACTGATGGAGCTCCTTCGGGAGAAAGAGGAGAGGATTCTGGCTCTGGAAGCTGA
  TATGACAAAGTGGGAGCAGAAATATTTGGAGGAGAATGTGATGAGACATTTTGCTCTGGATGCTGCTGCA
  ACTGTGGCTGCTCAGAGGGACACAACAGTCATCAGTCACTCTCCTAACACCAGCTATGACACAGCTCTAG
  AAGCTCGCATCCAGAAAGAGGAGGAAGAAATCTTGATGGCCAATAAGCGTTGCCTTGACATGGAGGGCAG
  GATTAAGACCCTCCATGCCCAGATTATTGAGAAGGATGCCATGATCAAAGTACTCCAGCAGCGTTCCCGG
  AAGGAGCCGAGCAAGACAGAGCAGCTGTCGTGCATGCGGCCAGCGAAGTCTCTGATGTCCATTTCCAATG
  CTGGATCAGGCTTGCTCTCCCACTCATCCACCCTGACTGGCTCCCCCATCATGGAAGAAAAGCGAGACGA
  CAAGAGCTGGAAGGGGAGCCTAGGCATTCTCCTGGGTGGAGACTACCGTGCTGAATATGTCCCTTCCACA
  CCCTCGCCTGTGCCACCCTCGACTCCCCTGCTCTCGGCTCACTCCAAGACAGGCAGCCGAGACTGCAGTA
  CCCAAACTGAACGTGGGACGGAATCGAACAAAACTGCAGCTGTTGCTCCCATCTCTGTTCCTGCTCCAGT
  TGCTGCTGCCGCCACTGCTGCCGCCATCACTGCCACTGCTGCCACCATCACCACCACCATGGTAGCTGCT
  GCTCCAGTTGCTGTTGCTGCTGCTGCTGCTCCAGCTGCTGCTGCTGCCCCGTCTCCAGCCACTGCCGCTG
  CTACTGCTGCTGCTGTTTCTCCAGCTGCTGCTGGTCAGATTCCAGCTGCTGCCTCTGTTGCCTCAGCTGC
  TGCCGTTGCTCCTTCTGCTGCTGCTGCTGCTGCTGTTCAGGTTGCTCCAGCTGCTCCGGCTCCAGTTCCA
  GCTCCGGCTCTGGTTCCGGTTCCAGCTCCAGCAGCGGCTCAGGCTTCTGCTCCTGCTCAGACTCAGGCAC
  CAACTTCAGCTCCGGCTGTGGCTCCAACTCCAGCTCCAACTCCAACTCCAGCTGTGGCTCAGGCTGAGGT
  TCCTGCAAGTCCAGCTACCGGTCCTGGACCACATCGTTTGTCTATACCAAGTTTGACCTGCAATCCAGAC
  AAAACAGATGGGCCTGTGTTCCACTCCAATACTCTGGAAAGAAAAACTCCCATTCAGATCCTGGGACAAG
  AGCCTGATGCAGAGATGGTGGAATATCTCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226319 representing NM_001113490
  Red=Cloning site Green=Tags(s)
  
  MRNSEEQPSGGTTVLQRLLQEQLRYGNPSENRSLLAIHQQATGNGPPFPSGSGNPGPQSDVLSPQDHHQQ
  LVAHAARQEPQGQEIQSENLIMEKQLSPRMQNNEELPTYEEAKVQSQYFRGQQHASVGAAFYVTGVTNQK
  MRTEGRPSVQRLNPGKMHQDEGLRDLKQGHVRSLSERLMQMSLATSGVKAHPPVTSAPLSPPQPNDLYKN
  PTSSSEFYKAQGPLPNQHSLKGMEHRGPPPEYPFKGMPPQSVVCKPQEPGHFYSEHRLNQPGRTEGQLMR
  YQHPPEYGAARPAQDISLPLSARNSQPHSPTSSLTSGGSLPLLQSPPSTRLSPARHPLVPNQGDHSAHLP
  RPQQHFLPNQAHQGDHYRLSQPGLSQQQQQQQQQHHHHHHHQQQQQQQPQQQPGEAYSAMPRAQPSSASY
  QPVPADPFAIVSRAQQMVEILSDENRNLRQELEGCYEKVARLQKVETEIQRVSEAYENLVKSSSKREALE
  KAMRNKLEGEIRRMHDFNRDLRERLETANKQLAEKEYEGSEDTRKTISQLFAKNKESQREKEKLEAELAT
  ARSTNEDQRRHIEIRDQALSNAQAKVVKLEEELKKKQVYVDKVEKMQQALVQLQAACEKREQLEHRLRTR
  LERELESLRIQQRQGNCQPTNVSEYNAAALMELLREKEERILALEADMTKWEQKYLEENVMRHFALDAAA
  TVAAQRDTTVISHSPNTSYDTALEARIQKEEEEILMANKRCLDMEGRIKTLHAQIIEKDAMIKVLQQRSR
  KEPSKTEQLSCMRPAKSLMSISNAGSGLLSHSSTLTGSPIMEEKRDDKSWKGSLGILLGGDYRAEYVPST
  PSPVPPSTPLLSAHSKTGSRDCSTQTERGTESNKTAAVAPISVPAPVAAAATAAAITATAATITTTMVAA
  APVAVAAAAAPAAAAAPSPATAAATAAAVSPAAAGQIPAAASVASAAAVAPSAAAAAAVQVAPAAPAPVP
  APALVPVPAPAAAQASAPAQTQAPTSAPAVAPTPAPTPTPAVAQAEVPASPATGPGPHRLSIPSLTCNPD
  KTDGPVFHSNTLERKTPIQILGQEPDAEMVEYLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113490
• ORF Size: 3252 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113490.1, NP_001106962.1
• RefSeq ORF: 3255 bp
• Locus ID: 154796
• UniProt ID: Q4VCS5
• Cytogenetics: Xq23
• MW: 117.9 kDa
• Gene Summary: This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]