ARMC5 (NM_001105247) Human Tagged ORF Clone

CAT#: RC226267

ARMC5 (Myc-DDK-tagged)-Human armadillo repeat containing 5 (ARMC5), transcript variant 1

Product Images

Circular map for RC226267

• 

  Western blot validation of overexpression lysate (Cat# LY426234) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226267 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARMC5
• Synonyms: AIMAH2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226267 representing NM_001105247
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGCGAAGCCAACCCTCACGGACTCGCTCTCGTTCTGCCTCGCGCAGCTCGCGGCGGCGGCCG
  GGGAGGCTCTGGGTGGGGAAAAGGACCCAGCGACCAACGAGACACCCCTGAGCCGCGCGCTCCTAGCCCT
  CCGCACGCGCCACATCAAGGCAGCGGGGGGAATCGAGCGCTTCCGGGCACGCGGCGGGCTCCGCCCCCTA
  CTCGCGCTGCTACGGCGAGCGGCTGCAGCGGGTTCCGCCCCGTCCCAGGCAGGCCCCGGCTCCGCCCCCT
  CGTCGGCCGCGTCGGGAGCTTCTAGCCCCGCCCCCGCGTCGGGCCCCGCCCCCTCCGCTGTGTCGTCGTC
  TAGTCCTACGCCGCCAGTGCGCCTGCGCAAGACGCTGGACTTGGCGCTCAGCATCCTAGCCGATTGCTGT
  ACGGAAGGGGCGTGCCGGACCGAAGTGCGCAGACTCGGAGGCATACTCCCTTTGGTGACCATTCTTCAGT
  GCATGAAGACAGACAGCATCCAGAACCGAACGGCCCGTGCCCTGGGGAACTTAGCCATGGAACCTGAGAG
  CTGTGGGGACATCCACTGTGCTGGTGCTGTTCCCCTGCTTGTGGAGAGCCTGACAGCCTGCCAGGACTCG
  CAGTGCCTACAGAGCGTGGTGCGTGCCCTCCGTAACCTGGCAGACTCACCCCAGCACCGCCTGGCCTTGG
  CACAGCAGGGAGCAGTGCGTCCGCTGGCCGAGCTCCTGGCCACTGCCCCAGATGCTGCACTGACCTTAGC
  CCTCGTCCGTGCCCTCCTGGAACTCAGCCGAGGCTGCTCCCGGGCCTGTGCTGAGCAGCTAAGTCTGGGT
  GGGGGATTGGGCCCACTCGTCAGCCTGGCTTCCCACCCCAAGCGGGCAGTACGCGAGGGAACCATTCTGA
  TCCTCGCCAACCTGTGTGCCCAGGGCCTGATTCGGCCTGCACTGGGCAATGCTGGTGGCGTGGAGGTGCT
  GGTAGATGAGCTCCGGCAGCGCCGGGATCCTAATGGAGCTAGCCCAACCTCCCAGCAGCCCCTGGTGCGG
  GCTGTGTGCCTCCTATGTCGTGAGGCCATCAACCGGGCCCGACTGCGGGATGCTGGTGGCTTGGATCTAC
  TGATGGGCCTGCTGCGGGACCCTCGTGCAAGCGCATGGCACCCTCGTATTGTGGCTGCCCTTGTGGGGTT
  TCTGTATGACACTGGGGCCCTGGGCCGGCTGCAGGCTCTGGGACTTGTGCCTCTCCTGGCTGGGCAGCTG
  TGTGGTGAGGCTGGTGAGGAGGAAGAAGAGGGAAGAGAAGCTGCTTCCTGGGACTTTCCTGAGGAGAGGA
  CCCCTGAGCGGGCACAGGGTGGAAGCTTCCGGAGCCTCAGGTCGTGGCTGATCTCCGAGGGCTATGCCAC
  AGGCCCTGATGACATCTCCCCCGACTGGTCTCCTGAGCAGTGTCCGCCGGAGCCCATGGAGCCGGCCAGC
  CCCGCCCCGACCCCGACCTCGCTGCGGGCACCACGCACCCAACGCACTCCGGGCCGCAGCCCCGCCGCCG
  CCATCGAGGAGCCTTGGGGACGCGAAGGGCCAGCCCTGCTGCTGCTGTCGCGCTTTTCCCAGGCCCCTGA
  CCCAAGTGGGGCACTTGTGACCGGCCCGGCGCTGTACGGCCTGCTGACCTATGTGACCGGCGCACCGGGC
  CCGCCCAGCCCACGTGCACTGCGCATTCTGTCACGCCTCACCTGCAACCCTGCCTGCCTCGAGGCCTTCG
  TGCGCAGCTATGGCGCGGCGCTGCTGCGGGCCTGGCTGGTGCTGGGGGTGGCGCCTGACGATTGGCCGGC
  ACCACGTGCCCGGCCCACTCTCCACAGCCGGCACCGAGAGCTGGGGGAGAGGCTACTGCAGAACCTGACG
  GTTCAGGCTGAGTCGCCCTTTGGGGTTGGGGCCCTGACGCACCTGCTGCTCTCTGGGAGCCCTGAGGACC
  GAGTGGCCTGCGCGCTGACCCTGCCCTTCATCTGCCGGAAGCCCTCTCTGTGGCGCCGGCTGCTTCTGGA
  GCAGGGTGGTCTCCGGCTCCTCCTTGCGGCGCTGACCCGGCCGGCCCCACACCCGCTCTTCCTCTTCTTT
  GCCGCGGACTCCCTTTCCTGCCTCCAAGACCTGGTGTCTCCCACTGTGAGCCCAGCTGTCCCACAGGCAG
  TCCCCATGGACCTAGACTCACCTTCCCCTTGCCTCTATGAACCTCTGCTGGGCCCAGCCCCTGTCCCAGC
  TCCCGACCTGCACTTCCTGCTGGACTCAGGCCTCCAGCTCCCTGCCCAGCGAGCGGCCTCAGCCACCGCC
  TCCCCTTTCTTCCGGGCCCTGCTGTCAGGCAGCTTTGCAGAAGCCCAGATGGACCTGGTGCCCCTGCGAG
  GTCTGTCGCCTGGTGCAGCCTGGCCTGTCCTGCATCATTTGCATGGTTGTCGGGGGTGTGGGGCTGCCCT
  GGGGCCCGTGCCCCCACCAGGCCAGCCCCTGCTGGGTTCAGAGGCCGAGGAGGCACTGGAGGCTGCTGGC
  CGTTTCCTACTGCCTGGGCTGGAGGAGGAGCTGGAAGAGGCCGTGGGCCGCATCCACCTGGGACCCCAGG
  GTGGCCCGGAGTCAGTGGGTGAGGTGTTCCGCCTGGGCCGGCCCCGGCTGGCTGCCCACTGTGCCCGCTG
  GACACTGGGGTCAGAGCAGTGCCCGAGGAAGCGGGGTCTGGCCCTGGTGGGGCTTGTGGAGGCAGCAGGT
  GAAGAGGCAGGGCCCCTGACGGAGGCTTTGCTGGCTGTGGTGATGGGGATTGAGTTGGGGGCAAGGGTCC
  CTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226267 representing NM_001105247
  Red=Cloning site Green=Tags(s)
  
  MAAAKPTLTDSLSFCLAQLAAAAGEALGGEKDPATNETPLSRALLALRTRHIKAAGGIERFRARGGLRPL
  LALLRRAAAAGSAPSQAGPGSAPSSAASGASSPAPASGPAPSAVSSSSPTPPVRLRKTLDLALSILADCC
  TEGACRTEVRRLGGILPLVTILQCMKTDSIQNRTARALGNLAMEPESCGDIHCAGAVPLLVESLTACQDS
  QCLQSVVRALRNLADSPQHRLALAQQGAVRPLAELLATAPDAALTLALVRALLELSRGCSRACAEQLSLG
  GGLGPLVSLASHPKRAVREGTILILANLCAQGLIRPALGNAGGVEVLVDELRQRRDPNGASPTSQQPLVR
  AVCLLCREAINRARLRDAGGLDLLMGLLRDPRASAWHPRIVAALVGFLYDTGALGRLQALGLVPLLAGQL
  CGEAGEEEEEGREAASWDFPEERTPERAQGGSFRSLRSWLISEGYATGPDDISPDWSPEQCPPEPMEPAS
  PAPTPTSLRAPRTQRTPGRSPAAAIEEPWGREGPALLLLSRFSQAPDPSGALVTGPALYGLLTYVTGAPG
  PPSPRALRILSRLTCNPACLEAFVRSYGAALLRAWLVLGVAPDDWPAPRARPTLHSRHRELGERLLQNLT
  VQAESPFGVGALTHLLLSGSPEDRVACALTLPFICRKPSLWRRLLLEQGGLRLLLAALTRPAPHPLFLFF
  AADSLSCLQDLVSPTVSPAVPQAVPMDLDSPSPCLYEPLLGPAPVPAPDLHFLLDSGLQLPAQRAASATA
  SPFFRALLSGSFAEAQMDLVPLRGLSPGAAWPVLHHLHGCRGCGAALGPVPPPGQPLLGSEAEEALEAAG
  RFLLPGLEEELEEAVGRIHLGPQGGPESVGEVFRLGRPRLAAHCARWTLGSEQCPRKRGLALVGLVEAAG
  EEAGPLTEALLAVVMGIELGARVPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001105247
• ORF Size: 2805 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001105247.2
• RefSeq ORF: 2808 bp
• Locus ID: 79798
• UniProt ID: Q96C12
• Cytogenetics: 16p11.2
• MW: 97.5 kDa
• Gene Summary: This gene encodes a member of the ARM (armadillo/beta-catenin-like repeat) superfamily. The ARM repeat is a tandemly repeated sequence motif with approximately 40 amino acid long. This repeat is implicated in mediating protein-protein interactions. The encoded protein contains seven ARM repeats. Mutations in this gene are associated with primary bilateral macronodular adrenal hyperplasia, which is also known as ACTH-independent macronodular adrenal hyperplasia 2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]