CLC7 (CLCN7) (NM_001114331) Human Tagged ORF Clone

CAT#: RC226163

CLCN7 (Myc-DDK-tagged)-Human chloride channel 7 (CLCN7), transcript variant 2

Product Images

Circular map for RC226163

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CLC7
• Synonyms: CLC-7; CLC7; HOD; OPTA2; OPTB4; PPP1R63
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226163 representing NM_001114331
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAACGTCTCTAAGAAGGTGTCCTGGTCCGGCCGGGACCGGGACGACGAGGAGGCGGCGCCGCTGC
  TGCGGAGGACGGCGCGGCCCGGCGGGGGGACGCCGCTGCTGAACGGGGCTGGGCCTGGGGCTGCGCGCCA
  GGATATGGACCCTCCACATCCCTTCCCCAAGGAGATCCCACACAACGAGAAGCTCCTGTCCCTCAAGTAT
  GAGAGCTTGGACTATGACAACAGTGAGAACCAGCTGTTCCTGGAGGAGGAGCGGCGGATCAATCACACGG
  CCTTCCGGACGGTGGAGATCAAGCGCTGGGTCATCTGCGCCCTCATTGGGATCCTCACGGGCCTCGTGGC
  CTGCTTCATTGACATCGTGGTGGAAAACCTGGCTGGCCTCAAGTACAGGGTCATCAAGGGCAATATCGAC
  AAGTTCACAGAGAAGGGCGGACTGTCCTTCTCCCTGTTGCTGTGGGCCACGCTGAACGCCGCCTTCGTGC
  TCGTGGGCTCTGTGATTGTGGCTTTCATAGAGCCGGTGGCTGCTGGCAGCGGAATCCCCCAGATCAAGTG
  CTTCCTCAACGGGGTGAAGATCCCCCACGTGGTGCGGCTCAAGACGTTGGTGATCAAAGTGTCCGGTGTG
  ATCCTGTCCGTGGTCGGGGGCCTGGCCGTGGGAAAGGAAGGGCCGATGATCCACTCAGGTTCAGTGATTG
  CCGCCGGGATCTCTCAGGGAAGGTCAACGTCACTGAAACGAGATTTCAAGATCTTCGAGTACTTCCGCAG
  AGACACAGAGAAGCGGGACTTCGTCTCCGCAGGGGCTGCGGCCGGAGTGTCAGCGGCGTTTGGAGCCCCC
  GTGGGTGGGGTCCTGTTCAGCTTGGAGGAGGGTGCGTCCTTCTGGAACCAGTTCCTGACCTGGAGGATCT
  TCTTTGCTTCCATGATCTCCACGTTCACCCTGAATTTTGTTCTGAGCATTTACCACGGGAACATGTGGGA
  CCTGTCCAGCCCAGGCCTCATCAACTTCGGAAGGTTTGACTCGGAGAAAATGGCCTACACGATCCACGAG
  ATCCCGGTCTTCATCGCCATGGGCGTGGTGGGCGGTGTGCTTGGAGCAGTGTTCAATGCCTTGAACTACT
  GGCTGACCATGTTTCGAATCAGGTACATCCACCGGCCCTGCCTGCAGGTGATTGAGGCCGTGCTGGTGGC
  CGCCGTCACGGCCACAGTTGCCTTCGTGCTGATCTACTCGTCGCGGGATTGCCAGCCCCTGCAGGGGGGC
  TCCATGTCCTACCCGCTGCAGCTCTTTTGTGCAGATGGCGAGTACAACTCCATGGCTGCGGCCTTCTTCA
  ACACCCCGGAGAAGAGCGTGGTGAGCCTCTTCCACGACCCGCCAGGCTCCTACAACCCCCTGACCCTCGG
  CCTGTTCACGCTGGTCTACTTCTTCCTGGCCTGCTGGACCTACGGGCTCACGGTGTCTGCCGGGGTCTTC
  ATCCCGTCCCTGCTCATCGGGGCTGCCTGGGGCCGGCTCTTTGGGATCTCCCTGTCCTACCTCACGGGGG
  CGGCGATCTGGGCGGACCCCGGCAAATACGCCCTGATGGGAGCTGCTGCCCAGCTGGGCGGGATTGTGCG
  GATGACACTGAGCCTGACCGTCATCATGATGGAGGCCACCAGCAACGTGACCTACGGCTTCCCCATCATG
  CTGGTGCTCATGACCGCCAAGATCGTGGGCGACGTCTTCATTGAGGGCCTGTACGACATGCACATTCAGC
  TGCAGAGTGTGCCCTTCCTGCACTGGGAGGCCCCGGTCACCTCACACTCACTCACTGCCAGGGAGGTGAT
  GAGCACACCAGTGACCTGCCTGAGGCGGCGTGAGAAGGTCGGCGTCATTGTGGACGTGCTGAGCGACACG
  GCGTCCAATCACAACGGCTTCCCCGTGGTGGAGCATGCCGATGACACCCAGCCTGCCCGGCTCCAGGGCC
  TGATCCTGCGCTCCCAGCTCATCGTTCTCCTAAAGCACAAGGTGTTTGTGGAGCGGTCCAACCTGGGCCT
  GGTACAGCGGCGCCTGAGGCTGAAGGACTTCCGAGACGCCTACCCGCGCTTCCCACCCATCCAGTCCATC
  CACGTGTCCCAGGACGAGCGGGAGTGCACCATGGACCTCTCCGAGTTCATGAACCCCTCCCCCTACACGG
  TGCCCCAGGAGGCGTCGCTCCCACGGGTGTTCAAGCTGTTCCGGGCCCTGGGCCTGCGGCACCTGGTGGT
  GGTGGACAACCGCAATCAGGTTGTCGGGTTGGTGACCAGGAAGGACCTCGCCAGGTACCGCCTGGGAAAG
  AGAGGCTTGGAGGAGCTCTCGCTGGCCCAGACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226163 representing NM_001114331
  Red=Cloning site Green=Tags(s)
  
  MANVSKKVSWSGRDRDDEEAAPLLRRTARPGGGTPLLNGAGPGAARQDMDPPHPFPKEIPHNEKLLSLKY
  ESLDYDNSENQLFLEEERRINHTAFRTVEIKRWVICALIGILTGLVACFIDIVVENLAGLKYRVIKGNID
  KFTEKGGLSFSLLLWATLNAAFVLVGSVIVAFIEPVAAGSGIPQIKCFLNGVKIPHVVRLKTLVIKVSGV
  ILSVVGGLAVGKEGPMIHSGSVIAAGISQGRSTSLKRDFKIFEYFRRDTEKRDFVSAGAAAGVSAAFGAP
  VGGVLFSLEEGASFWNQFLTWRIFFASMISTFTLNFVLSIYHGNMWDLSSPGLINFGRFDSEKMAYTIHE
  IPVFIAMGVVGGVLGAVFNALNYWLTMFRIRYIHRPCLQVIEAVLVAAVTATVAFVLIYSSRDCQPLQGG
  SMSYPLQLFCADGEYNSMAAAFFNTPEKSVVSLFHDPPGSYNPLTLGLFTLVYFFLACWTYGLTVSAGVF
  IPSLLIGAAWGRLFGISLSYLTGAAIWADPGKYALMGAAAQLGGIVRMTLSLTVIMMEATSNVTYGFPIM
  LVLMTAKIVGDVFIEGLYDMHIQLQSVPFLHWEAPVTSHSLTAREVMSTPVTCLRRREKVGVIVDVLSDT
  ASNHNGFPVVEHADDTQPARLQGLILRSQLIVLLKHKVFVERSNLGLVQRRLRLKDFRDAYPRFPPIQSI
  HVSQDERECTMDLSEFMNPSPYTVPQEASLPRVFKLFRALGLRHLVVVDNRNQVVGLVTRKDLARYRLGK
  RGLEELSLAQT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001114331
• ORF Size: 2343 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001114331.2, NP_001107803.1
• RefSeq Size: 4164 bp
• RefSeq ORF: 2346 bp
• Locus ID: 1186
• UniProt ID: P51798
• Cytogenetics: 16p13.3
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• MW: 86 kDa
• Gene Summary: The product of this gene belongs to the CLC chloride channel family of proteins. Chloride channels play important roles in the plasma membrane and in intracellular organelles. This gene encodes chloride channel 7. Defects in this gene are the cause of osteopetrosis autosomal recessive type 4 (OPTB4), also called infantile malignant osteopetrosis type 2 as well as the cause of autosomal dominant osteopetrosis type 2 (OPTA2), also called autosomal dominant Albers-Schonberg disease or marble disease autosoml dominant. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. OPTA2 is the most common form of osteopetrosis, occurring in adolescence or adulthood. [provided by RefSeq, Jul 2008]