NEDD1 (NM_001135175) Human Tagged ORF Clone

CAT#: RC226079

NEDD1 (Myc-DDK-tagged)-Human neural precursor cell expressed, developmentally down-regulated 1 (NEDD1), transcript variant 1

Product Images

Circular map for RC226079

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NEDD1
• Synonyms: GCP-WD; TUBGCP7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226079 representing NM_001135175
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATTTTACAGGCGCAGTCATGCAGGAAAACCTCAGATTTGCTTCATCAGGAGATGATATTAAAATAT
  GGGATGCTTCATCTATGACATTGGTGGATAAATTCAACCCACACACATCACCACATGGAATCAGCTCAAT
  ATGTTGGAGCAGCAATAATAACTTTTTAGTAACAGCATCTTCCAGTGGCGACAAAATAGTTGTCTCAAGT
  TGCAAATGTAAACCTGTTCCACTTTTAGAGCTTGCTGAAGGGCAAAAGCAGACATGTGTCAATTTAAATT
  CTACATCTATGTATTTGGTAAGCGGAGGCCTAAATAACACTGTTAATATTTGGGATTTAAAATCAAAAAG
  AGTTCATCGATCTCTTAAGGATCATAAAGATCAAGTAACTTGTGTAACATACAATTGGAATGATTGCTAC
  ATTGCTTCTGGATCTCTTAGTGGTGAAATTATTTTACACAGTGTAACCACTAATTTATCTAGTACTCCTT
  TTGGCCATGGTAGTAACCAGTCTGTTCGGCACTTGAAGTACTCCTTGTTTAAGAAATCACTACTGGGCAG
  TGTTTCGGATAATGGAATAGTAACTCTCTGGGATGTAAATAGTCAGAGTCCATACCATAACTTTGACAGT
  GTACACAAAGCTCCAGCGTCAGGCATCTGTTTTTCTCCTGTCAATGAATTGCTCTTTGTAACCATAGGCT
  TGGATAAAAGAATCATCCTCTATGACACTTCAAGTAAGAAGCTAGTGAAAACTTTAGTGGCTGACACTCC
  TCTAACTGCGGTAGATTTCATGCCTGATGGAGCCACTTTGGCTATTGGATCTTCCCGGGGGAAAATATAT
  CAATATGATTTAAGAATGTTGAAATCACCAGTTAAGACCATCAGTGCTCACAAGACATCTGTGCAGTGTA
  TAGCATTTCAGTACTCCACTGTTCTTACTAAGTCAAGTTTAAATAAAGGCTGTTCAAATAAGCCCACAAC
  AGTGAACAAACGAAGTGTTAATGTGAATGCTGCTAGTGGAGGAGTTCAGAATTCCGGAATTGTCAGAGAA
  GCACCTGCCACGTCCATTGCCACAGTTCTACCACAACCTATGACATCAGCTATGGGGAAAGGAACAGTTG
  CTGTTCAAGAAAAAGCAGGTTTGCCTCGAAGCATAAACACAGACACTTTATCTAAGGAAACAGACAGTGG
  AAAAAATCAGGATTTCTCCAGCTTTGATGATACTGGGAAAAGTAGTTTAGGTGACATGTTCTCACCTATC
  AGAGATGATGCTGTAGTTAACAAGGGAAGTGATGAGTCCATAGGCAAAGGAGATGGCTTTGACTTTCTAC
  CGCAGTTGAACTCAGTGTTTCCTCCAAGAAAAAATCCAGTAACTTCAAGTACTTCAGTATTGCATTCTAG
  TCCTCTTAATGTTTTTATGGGATCTCCAGGGAAAGAGGAAAATGAAAACCGTGATCTAACAGCTGAGTCT
  AAGAAAATATATATGGGAAAACAGGAATCTAAAGACTCCTTCAAACAGTTAGCAAAGTTGGTCACATCTG
  GTGCTGAAAGTGGAAATCTAAATACCTCTCCATCATCTAACCAAACAAGAAATTCTGAGAAATTTGAAAA
  GCCAGAGAATGAAATTGAAGCCCAGTTGATATGTGAACCCCCAATCAATGGATCCTCAACTCCAAATCCA
  AAGATAGCATCTTCTGTCACTGCTGGAGTTGCCAGTTCACTCTCAGAAAAAATAGCCGACAGCATTGGAA
  ATAACCGGCAAAATGCACCATTGACTTCCATTCAAATTCGTTTTATTCAGAACATGATACAGGAAACGTT
  GGATGACTTTAGAGAAGCATGCCATAGGGACATTGTGAATTTGCAAGTGGAGATGATTAAACAGTTTCAT
  ATGCAACTGAATGAAATGCATTCTTTGCTGGAAAGATACTCAGTGAATGAAGGTTTAGTGGCTGAAATTG
  AAAGACTACGAGAAGAAAACAAAAGATTACGGGCCCACTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226079 representing NM_001135175
  Red=Cloning site Green=Tags(s)
  
  MHFTGAVMQENLRFASSGDDIKIWDASSMTLVDKFNPHTSPHGISSICWSSNNNFLVTASSSGDKIVVSS
  CKCKPVPLLELAEGQKQTCVNLNSTSMYLVSGGLNNTVNIWDLKSKRVHRSLKDHKDQVTCVTYNWNDCY
  IASGSLSGEIILHSVTTNLSSTPFGHGSNQSVRHLKYSLFKKSLLGSVSDNGIVTLWDVNSQSPYHNFDS
  VHKAPASGICFSPVNELLFVTIGLDKRIILYDTSSKKLVKTLVADTPLTAVDFMPDGATLAIGSSRGKIY
  QYDLRMLKSPVKTISAHKTSVQCIAFQYSTVLTKSSLNKGCSNKPTTVNKRSVNVNAASGGVQNSGIVRE
  APATSIATVLPQPMTSAMGKGTVAVQEKAGLPRSINTDTLSKETDSGKNQDFSSFDDTGKSSLGDMFSPI
  RDDAVVNKGSDESIGKGDGFDFLPQLNSVFPPRKNPVTSSTSVLHSSPLNVFMGSPGKEENENRDLTAES
  KKIYMGKQESKDSFKQLAKLVTSGAESGNLNTSPSSNQTRNSEKFEKPENEIEAQLICEPPINGSSTPNP
  KIASSVTAGVASSLSEKIADSIGNNRQNAPLTSIQIRFIQNMIQETLDDFREACHRDIVNLQVEMIKQFH
  MQLNEMHSLLERYSVNEGLVAEIERLREENKRLRAHF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001135175
• ORF Size: 2001 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001135175.1, NP_001128647.1
• RefSeq Size: 3805 bp
• RefSeq ORF: 2004 bp
• Locus ID: 121441
• UniProt ID: Q8NHV4
• Cytogenetics: 12q23.1
• MW: 72.7 kDa
• Gene Summary: Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.[UniProtKB/Swiss-Prot Function]