SPARCL1 (NM_001128310) Human Tagged ORF Clone

CAT#: RC226078

SPARCL1 (Myc-DDK-tagged)-Human SPARC-like 1 (hevin) (SPARCL1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY426947) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226078 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SPARCL1 protein (Cat# TP326078). The protein was produced from HEK293T cells transfected with SPARCL1 cDNA clone (Cat# RC226078) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPARCL1
• Synonyms: MAST 9; MAST9; PIG33; SC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226078 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACTGGGCTTTTTTTCCTATGTCTCTTGGGAACTGCAGCTGCAATCCCGACAAATGCAAGATTAT
  TATCTGATCATTCCAAACCAACTGCTGAAACGGTAGCACCCGACAACACTGCAATCCCCAGTTTAAGGGC
  TGAAGATGAAGAAAATGAAAAAGAAACAGCAGTATCCACAGAAGACGATTCCCACCATAAGGCTGAAAAA
  TCATCAGTACTAAAGTCAAAAGAGGAAAGCCATGAACAGTCAGCAGAACAGGGCAAGAGTTCTAGCCAAG
  AGCTGGGATTGAAGGATCAAGAGGACAGTGATGGTGACTTAAGTGTGAATTTGGAGTATGCACCAACTGA
  AGGTACATTGGACATAAAAGAAGATATGAGTGAGCCTCAGGAGAAAAAACTCTCAGAGAACACTGATTTT
  TTGGCTCCTGGTGTTAGTTCCTTCACAGATTCTAACCAACAAGAAAGTATCACAAAGAGAGAGGAAAACC
  AAGAACAACCTAGAAATTATTCACATCATCAGTTGAACAGGAGCAGTAAACATAGCCAAGGCCTAAGGGA
  TCAAGGAAACCAAGAGCAGGATCCAAATATTTCCAATGGAGAAGAGGAAGAAGAAAAAGAGCCAGGTGAA
  GTTGGTACCCACAATGATAACCAAGAAAGAAAGACAGAATTGCCCAGGGAGCATGCTAACAGCAAGCAGG
  AGGAAGACAATACCCAATCTGATGATATTTTGGAAGAGTCTGATCAACCAACTCAAGTAAGCAAGATGCA
  GGAGGATGAATTTGATCAGGGTAACCAAGAACAAGAAGATAACTCCAATGCAGAAATGGAAGAGGAAAAT
  GCATCGAACGTCAATAAGCACATTCAAGAAACTGAATGGCAGAGTCAAGAGGGTAAAACTGGCCTAGAAG
  CTATCAGCAACCACAAAGAGACAGAAGAAAAGACTGTTTCTGAGGCTCTGCTCATGGAACCTACTGATGA
  TGGTAATACCACGCCCAGAAATCATGGAGTTGATGATGATGGCGATGATGATGGCGATGATGGCGGCACT
  GATGGCCCCAGGCACAGTGCAAGTGATGACTACTTCATCCCAAGCCAGGCCTTTCTGGAGGCCGAGAGAG
  CTCAATCCATTGCCTATCACCTCAAAATTGAGGAGCAAAGAGAAAAAGTACATGAAAATGAAAATATAGG
  TACCACTGAGCCTGGAGAGCACCAAGAGGCCAAGAAAGCAGAGAACTCATCAAATGAGGAGGAAACGTCA
  AGTGAAGGCAACATGAGGGTGCATGCTGTGGATTCTTGCATGAGCTTCCAGTGTAAAAGAGGCCACATCT
  GTAAGGCAGACCAACAGGGAAAACCTCACTGTGTCTGCCAGGATCCAGTGACTTGTCCTCCAACAAAACC
  CCTTGATCAAGTTTGTGGCACTGACAATCAGACCTATGCTAGTTCCTGTCATCTATTCGCTACTAAATGC
  AGACTGGAGGGGACCAAAAAGGGGCATCAACTCCAGCTGGATTATTTTGGAGCCTGCAAATCTATTCCTA
  CTTGTACGGACTTTGAAGTGATTCAGTTTCCTCTACGGATGAGAGACTGGCTCAAGAATATCCTCATGCA
  GCTTTATGAAGCCAACTCTGAACACGCTGGTTATCTAAATGAGAAGCAGAGAAATAAAGTCAAGAAAATT
  TACCTGGATGAAAAGAGGCTTTTGGCTGGGGACCATCCCATTGACCTTCTCTTAAGGGACTTTAAGAAAA
  ACTACCACATGTATGTGTATCCTGTGCACTGGCAGTTTAGTGAACTTGACCAACACCCTATGGATAGAGT
  CTTGACACATTCTGAACTTGCTCCTCTGCGAGCATCTCTGGTGCCCATGGAACACTGCATAACCCGTTTC
  TTTGAGGAGTGTGACCCCAACAAGGATAAGCACATCACCCTGAAGGAGTGGGGCCACTGCTTTGGAATTA
  AAGAAGAGGACATAGATGAAAATCTCTTGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226078 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKTGLFFLCLLGTAAAIPTNARLLSDHSKPTAETVAPDNTAIPSLRAEDEENEKETAVSTEDDSHHKAEK
  SSVLKSKEESHEQSAEQGKSSSQELGLKDQEDSDGDLSVNLEYAPTEGTLDIKEDMSEPQEKKLSENTDF
  LAPGVSSFTDSNQQESITKREENQEQPRNYSHHQLNRSSKHSQGLRDQGNQEQDPNISNGEEEEEKEPGE
  VGTHNDNQERKTELPREHANSKQEEDNTQSDDILEESDQPTQVSKMQEDEFDQGNQEQEDNSNAEMEEEN
  ASNVNKHIQETEWQSQEGKTGLEAISNHKETEEKTVSEALLMEPTDDGNTTPRNHGVDDDGDDDGDDGGT
  DGPRHSASDDYFIPSQAFLEAERAQSIAYHLKIEEQREKVHENENIGTTEPGEHQEAKKAENSSNEEETS
  SEGNMRVHAVDSCMSFQCKRGHICKADQQGKPHCVCQDPVTCPPTKPLDQVCGTDNQTYASSCHLFATKC
  RLEGTKKGHQLQLDYFGACKSIPTCTDFEVIQFPLRMRDWLKNILMQLYEANSEHAGYLNEKQRNKVKKI
  YLDEKRLLAGDHPIDLLLRDFKKNYHMYVYPVHWQFSELDQHPMDRVLTHSELAPLRASLVPMEHCITRF
  FEECDPNKDKHITLKEWGHCFGIKEEDIDENLLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001128310
• ORF Size: 1992 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128310.2
• RefSeq Size: 3012 bp
• RefSeq ORF: 1995 bp
• Locus ID: 8404
• UniProt ID: Q14515
• Cytogenetics: 4q22.1
• Protein Families: Druggable Genome, Secreted Protein
• MW: 75.2 kDa