MSF (SEPT9) (NM_001113493) Human Tagged ORF Clone

USD 540.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name MSF (SEPT9) (NM_001113493) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SEPT9
Synonyms AF17q25; MSF; MSF1; NAPB; PNUTL4; SEPT9; SeptD1; SINT1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC225979 representing NM_001113493
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGACCCCGCGGTCAACGCGCAGCTGGATGGGATCATTTCGGACTTCGAAGCCTTGAAAAGATCTT
TTGAGGTCGAGGAGGTCGAGACACCCAACTCCACCCCACCCCGGAGGGTCCAGACTCCCCTACTCCGAGC
CACTGTGGCCAGCTCCACCCAGAAATTCCAGGACCTGGGCGTGAAGAACTCAGAACCCTCGGCCCGCCAT
GTGGACTCCCTAAGCCAACGCTCCCCCAAGGCGTCCCTGCGGAGGGTGGAGCTCTCGGGCCCCAAGGCGG
CCGAGCCGGTGTCCCGGCGCACTGAGCTGTCCATTGACATCTCGTCCAAGCAGGTGGAGAACGCCGGGGC
CATCGGCCCGTCCCGGTTCGGGCTCAAGAGGGCCGAGGTGTTGGGCCACAAGACGCCAGAACCGGCCCCT
CGGAGGACGGAGATCACCATCGTCAAACCCCAGGAGTCAGCCCACCGGAGGATGGAGCCCCCTGCCTCCA
AGGTCCCCGAGGTGCCCACTGCCCCTGCCACCGACGCAGCCCCCAAGAGGGTGGAGATCCAGATGCCCAA
GCCTGCTGAGGCGCCCACCGCCCCCAGCCCAGCCCAGACCTTGGAGAATTCAGAGCCTGCCCCTGTGTCT
CAGCTGCAGAGCAGGCTGGAGCCCAAGCCCCAGCCCCCTGTGGCTGAGGCTACACCCCGGAGCCAGGAGG
CCACTGAGGCGGCTCCCAGCTGCGTTGGCGACATGGCCGACACCCCCAGAGATGCCGGGCTCAAGCAGGC
GCCTGCATCACGGAACGAGAAGGCCCCGGTGGACTTCGGCTACGTGGGGATTGACTCCATCCTGGAGCAG
ATGCGCCGGAAGGCCATGAAGCAGGGCTTCGAGTTCAACATCATGGTGGTCGGGCAGAGCGGCTTGGGTA
AATCCACCTTAATCAACACCCTCTTCAAATCCAAAATCAGCCGGAAGTCGGTGCAGCCCACCTCAGAGGA
GCGCATCCCCAAGACCATCGAGATCAAGTCCATCACGCACGATATTGAGGAGAAAGGCGTCCGGATGAAG
CTGACAGTGATTGACACACCAGGGTTCGGGGACCACATCAACAACGAGAACTGCTGGCAGCCCATCATGA
AGTTCATCAATGACCAGTACGAGAAATACCTGCAGGAGGAGGTCAACATCAACCGCAAGAAGCGCATCCC
GGACACCCGCGTCCACTGCTGCCTCTACTTCATCCCCGCCACCGGCCACTCCCTCAGGCCCCTGGACATC
GAGTTTATGAAACGCCTGAGCAAGGTGGTCAACATCGTCCCTGTCATCGCCAAGGCGGACACACTCACCC
TGGAGGAGAGGGTCCACTTCAAACAGCGGATCACCGCAGACCTGCTGTCCAACGGCATCGACGTGTACCC
CCAGAAGGAATTTGATGAGGACTCGGAGGACCGGCTGGTGAACGAGAAGTTCCGGGAGATGATCCCATTT
GCTGTGGTGGGCAGTGACCACGAGTACCAGGTCAACGGCAAGAGGATCCTTGGGAGGAAGACCAAGTGGG
GTACCATCGAAGTTGAAAACACCACACACTGTGAGTTTGCCTACCTGCGGGACCTTCTCATCAGGACGCA
CATGCAGAACATCAAGGACATCACCAGCAGCATCCACTTCGAGGCGTACCGTGTGAAGCGCCTCAACGAG
GGCAGCAGCGCCATGGCCAACGGCATGGAGGAGAAGGAGCCAGAAGCCCCGGAGATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC225979 representing NM_001113493
Red=Cloning site Green=Tags(s)

MSDPAVNAQLDGIISDFEALKRSFEVEEVETPNSTPPRRVQTPLLRATVASSTQKFQDLGVKNSEPSARH
VDSLSQRSPKASLRRVELSGPKAAEPVSRRTELSIDISSKQVENAGAIGPSRFGLKRAEVLGHKTPEPAP
RRTEITIVKPQESAHRRMEPPASKVPEVPTAPATDAAPKRVEIQMPKPAEAPTAPSPAQTLENSEPAPVS
QLQSRLEPKPQPPVAEATPRSQEATEAAPSCVGDMADTPRDAGLKQAPASRNEKAPVDFGYVGIDSILEQ
MRRKAMKQGFEFNIMVVGQSGLGKSTLINTLFKSKISRKSVQPTSEERIPKTIEIKSITHDIEEKGVRMK
LTVIDTPGFGDHINNENCWQPIMKFINDQYEKYLQEEVNINRKKRIPDTRVHCCLYFIPATGHSLRPLDI
EFMKRLSKVVNIVPVIAKADTLTLEERVHFKQRITADLLSNGIDVYPQKEFDEDSEDRLVNEKFREMIPF
AVVGSDHEYQVNGKRILGRKTKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDITSSIHFEAYRVKRLNE
GSSAMANGMEEKEPEAPEM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_001113493
ORF Size 1737 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001113493.1, NP_001106965.1
RefSeq ORF 1740
Locus ID 10801
Cytogenetics 17q25.3
Protein Families Druggable Genome
MW 64.5 kDa
Gene Summary This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2009]

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