ART3 (NM_001130016) Human Tagged ORF Clone

CAT#: RC225592

ART3 (Myc-DDK-tagged)-Human ADP-ribosyltransferase 3 (ART3), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY427105) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225592 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ART3
• Synonyms: ARTC3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225592 representing NM_001130016
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACGGGACATTTTGAAATAGTCACCATGCTGCTGGCAACCATGATTCTAGTGGACATTTTCCAGG
  TGAAGGCTGAAGTGTTAGACATGGCAGATAATGCATTTGATGATGAATACCTGAAATGTACGGACAGGAT
  GGAAATTAAATACGTTCCCCAACTGCTAAAGGAGGAAAAAGCAAGCCACCAGCAATTAGATACTGTGTGG
  GAAAATGCAAAAGCCAAATGGGCAGCCCGAAAGACTCAAATCTTTCTCCCTATGAATTTTAAGGATAACC
  ATGGAATAGCCCTGATGGCATATATTTCCGAAGCTCAAGAGCAAACTCCCTTTTACCATCTGTTCAGTGA
  AGCTGTGAAGATGGCTGGCCAATCTCGAGAAGATTATATCTATGGCTTCCAGTTCAAAGCTTTCCACTTT
  TACCTCACAAGAGCCCTGCAGTTGCTGAGAAAACCTTGTGAGGCCAGTTCCAAAACTGTGGTATATAGAA
  CAAGCCAGGGCACTTCATTTACATTTGGAGGGCTAAACCAAGCCAGGTTTGGCCATTTTACCTTGGCATA
  TTCAGCCAAACCTCAGGCTGCTAATGACCAGCTCACTGTGTTATCCATCTACACATGCCTTGGAGTTGAC
  ATTGAAAATTTTCTTGATAAAGAAAGTGAAAGAATTACTTTAATACCTCTGAATGAGGTTTTTCAAGTGT
  CACAGGAGGGGGCTGGCAATAACCTTATCCTTCAAAGCATAAACAAGACCTGCAGCCATTATGAGTGTGC
  ATTTCTAGGTGGACTAAAAACCGAAAACTGTATTGAGAACCTAGAATATTTTCAACCCATCTATGTCTAC
  AACCCTGGTGAGAAAAACCAGAAGCTTGAAGACCATAGTGAGAAAAACTGGAAGCTTGAAGACCATGGTG
  AGAAAAACCAGAAGCTTGAAGACCATGGTGTGAAAATCCTTGAACCCACCCAAATACCTGGAATGAAAAT
  TCCAGAACCTTTTCCACTACCTGAAGATAAAAGTCAAGGAAATATCAACAATCCTACTCCAGGTCCAGTT
  CCTGTTCCAGGTCCCAAAAGCCATCCTTCTGCATCCTTGGGCAAACTGCTGCTTCCACAGTTTGGGATGG
  TCATCATTTTAATCAGTGTTTCTGCTATAAATCTCTTTGTTGCTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225592 representing NM_001130016
  Red=Cloning site Green=Tags(s)
  
  MKTGHFEIVTMLLATMILVDIFQVKAEVLDMADNAFDDEYLKCTDRMEIKYVPQLLKEEKASHQQLDTVW
  ENAKAKWAARKTQIFLPMNFKDNHGIALMAYISEAQEQTPFYHLFSEAVKMAGQSREDYIYGFQFKAFHF
  YLTRALQLLRKPCEASSKTVVYRTSQGTSFTFGGLNQARFGHFTLAYSAKPQAANDQLTVLSIYTCLGVD
  IENFLDKESERITLIPLNEVFQVSQEGAGNNLILQSINKTCSHYECAFLGGLKTENCIENLEYFQPIYVY
  NPGEKNQKLEDHSEKNWKLEDHGEKNQKLEDHGVKILEPTQIPGMKIPEPFPLPEDKSQGNINNPTPGPV
  PVPGPKSHPSASLGKLLLPQFGMVIILISVSAINLFVAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130016
• ORF Size: 1167 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130016.3
• RefSeq ORF: 1170 bp
• Locus ID: 419
• UniProt ID: Q13508
• Cytogenetics: 4p15.1-p14
• Protein Families: Transmembrane
• MW: 43.7 kDa
• Gene Summary: This gene encodes an arginine-specific ADP-ribosyltransferase. The encoded protein catalyzes a reversible reaction which modifies proteins by the addition or removal of ADP-ribose to an arginine residue to regulate the function of the modified protein. An ADP-ribosyltransferase pseudogene is located on chromosome 11. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]