PSG5 (NM_001130014) Human Tagged ORF Clone

CAT#: RC225493

PSG5 (Myc-DDK-tagged)-Human pregnancy specific beta-1-glycoprotein 5 (PSG5), transcript variant 2

Product Images

Circular map for RC225493

• 

  Western blot validation of overexpression lysate (Cat# LY427103) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225493 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PSG5
• Synonyms: FL-NCA-3; PSG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225493 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCCCTCTCAGCCCCTCCCTGCACACAGCACATCACCTGGAAGGGGGTCCTGCTCACAGCATCAC
  TTTTAAACTTCTGGAACCTGCCTATCACTGCTCAAGTCACGATTGAAGCCCTGCCACCCAAAGTTTCCGA
  GGGGAAGGATGTTCTTCTACTTGTCCACAATTTGCCTCAGAATCTTGCTGGCTACATCTGGTACAAAGGA
  CAACTGATGGACCTCTACCATTACATTACATCATATGTAGTAGACGGTCAAATAAATATATATGGGCCTG
  CATACACTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCCAGAATGTCACCCGGGAAGACGC
  AGGATCCTATACCTTACACATCATAAAGCGAGGTGATAGGACTAGAGGAGTAACTGGATATTTCACCTTC
  AACTTATACCTGAAGCTGCCCAAGCCCTACATCACCATCAACAACTCAAAACCCAGGGAGAATAAGGATG
  TCTTAGCCTTCACCTGTGAACCTAAGAGTGAGAACTACACCTACATTTGGTGGCTAAATGGTCAGAGCCT
  CCCGGTCAGTCCCAGGGTAAAGCAACCCATTGAAAACAGGATCCTCATTCTACCCAGTGTCACGAGAAAT
  GAAACAGGACCCTATGAATGTGAAATACGGGACCGAGATGGTGGCATGCACAGTGACCCAGTCACCCTGA
  ATGTCCTCTATGGTCCAGACCTCCCCAGCATTTACCCTTCATTCACCTATTACCGTTCAGGAGAAAACCT
  CTACTTGTCCTGCTTCGCGGAATCTAACCCACCGGCAGAGTATTTTTGGACAATTAATGGGAAGTTTCAG
  CAATCAGGACAAAAGCTCTCTATCCCCCAAATTACTACAAAGCATAGAGGGCTCTATACTTGCTCTGTTC
  GTAACTCAGCCACTGGCAAGGAAAGCTCCAAATCCATGACAGTCGAAGTCTCTGCTCCTTCAGGAATAGG
  ACGTCTTCCTCTCCTTAATCCAATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225493 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGPLSAPPCTQHITWKGVLLTASLLNFWNLPITAQVTIEALPPKVSEGKDVLLLVHNLPQNLAGYIWYKG
  QLMDLYHYITSYVVDGQINIYGPAYTGRETVYSNASLLIQNVTREDAGSYTLHIIKRGDRTRGVTGYFTF
  NLYLKLPKPYITINNSKPRENKDVLAFTCEPKSENYTYIWWLNGQSLPVSPRVKQPIENRILILPSVTRN
  ETGPYECEIRDRDGGMHSDPVTLNVLYGPDLPSIYPSFTYYRSGENLYLSCFAESNPPAEYFWTINGKFQ
  QSGQKLSIPQITTKHRGLYTCSVRNSATGKESSKSMTVEVSAPSGIGRLPLLNPI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130014
• ORF Size: 1005 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130014.1, NP_001123486.1
• RefSeq Size: 1601 bp
• RefSeq ORF: 1008 bp
• Locus ID: 5673
• UniProt ID: Q15238
• Cytogenetics: 19q13.31
• Protein Families: Secreted Protein
• MW: 37.7 kDa
• Gene Summary: The human pregnancy-specific glycoproteins (PSGs) are a group of molecules that are mainly produced by the placental syncytiotrophoblasts during pregnancy. PSGs comprise a subgroup of the carcinoembryonic antigen (CEA) family, which belongs to the immunoglobulin superfamily. For additional general information about the PSG gene family, see PSG1 (MIM 176390).[supplied by OMIM, Oct 2009]