SIDT1 (NM_017699) Human Tagged ORF Clone

CAT#: RC224950

SIDT1 (Myc-DDK-tagged)-Human SID1 transmembrane family, member 1 (SIDT1)

Product Images

Western blot validation of overexpression lysate (Cat# LY413608) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224950 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SIDT1
• Synonyms: SID-1; SID1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224950 representing NM_017699
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGGCTGCCTGCGGCTCGCGCTGCTCTGCGCGCTGCCCTGGCTCCTGCTGGCGGCGTCGCCCGGGC
  ACCCGGCGAAATCCCCCAGGCAGCCCCCGGCACCGCGCCGCGACCCCTTCGACGCTGCCAGGGGCGCCGA
  TTTCGATCATGTCTACAGCGGGGTGGTGAACCTCAGCACCGAGAACATCTACTCTTTCAACTACACCAGC
  CAGCCCGACCAGGTGACAGCCGTGAGGGTGTATGTGAACAGTTCCTCTGAGAATCTCAACTACCCGGTCC
  TTGTTGTGGTTCGCCAGCAGAAAGAGGTGCTGTCCTGGCAGGTTCCTCTGCTCTTCCAAGGACTATACCA
  GAGGAGCTACAACTATCAAGAAGTGAGCCGCACCTTATGTCCCTCAGAAGCAACCAATGAGACGGGACCC
  TTGCAGCAACTGATATTTGTAGATGTCGCATCCATGGCACCCCTGGGTGCTCAGTACAAACTGCTAGTTA
  CCAAGCTGAAGCACTTCCAGCTCCGGACAAATGTTGCCTTTCACTTTACTGCCAGCCCCTCTCAACCTCA
  GTATTTTCTATACAAGTTTCCCAAAGACGTGGACTCAGTTATCATTAAAGTGGTGTCTGAAATGGCTTAT
  CCATGTTCTGTTGTCTCAGTCCAGAATATCATGTGCCCGGTGTATGATCTCGACCACAATGTGGAATTTA
  ATGGTGTCTATCAGTCCATGACCAAGAAAGCTGCCATCACGCTACAGAAGAAGGATTTTCCAGGCGAGCA
  GTTCTTCGTGGTATTTGTGATAAAGCCTGAAGATTATGCCTGTGGAGGATCTTTCTTCATCCAGGAAAAG
  GAAAACCAGACCTGGAATCTACAGCGAAAAAAGAACCTTGAAGTGACCATTGTCCCTTCCATTAAAGAAT
  CTGTTTATGTGAAATCCAGTCTTTTCAGTGTCTTCATCTTCCTGTCCTTCTACTTGGGATGCCTTCTTGT
  TGGGTTTGTTCATTATCTGAGGTTTCAGAGAAAATCCATTGATGGAAGCTTTGGGTCCAATGATGGCTCT
  GGAAATATGGTGGCATCTCATCCCATTGCTGCCAGCACACCCGAAGGGAGCAATTATGGGACAATAGATG
  AGTCAAGCTCCAGTCCTGGAAGGCAGATGTCCTCCTCCGATGGTGGGCCACCGGGCCAGTCAGACACAGA
  CAGCTCCGTGGAGGAGAGCGACTTCGACACCATGCCAGACATTGAGAGTGATAAAAACATCATCCGGACC
  AAGATGTTCCTTTACCTGTCAGATTTGTCCAGGAAGGACCGGAGAATTGTCAGCAAAAAATATAAAATTT
  ATTTTTGGAACATCATCACCATTGCTGTGTTTTACGCGCTGCCCGTGATCCAGCTGGTCATTACCTATCA
  GACAGTGGTAAATGTCACTGGCAACCAGGACATCTGTTACTACAACTTCCTCTGTGCTCACCCCTTGGGC
  GTCCTGAGTGCCTTCAACAACATTCTCAGCAATCTGGGCCACGTGCTTCTGGGCTTCCTCTTCCTGCTGA
  TAGTCTTGCGCCGCGACATCCTCCATCGGAGAGCCCTGGAAGCCAAGGACATCTTTGCTGTGGAGTACGG
  GATTCCCAAACACTTTGGTCTCTTCTACGCTATGGGCATTGCATTGATGATGGAAGGGGTGCTCAGTGCT
  TGCTACCATGTCTGCCCTAATTATTCCAACTTCCAATTCGACACCTCCTTCATGTACATGATCGCTGGCC
  TGTGCATGCTGAAGCTCTATCAGACCCGCCACCCAGACATCAATGCCAGCGCCTACTCTGCCTATGCCTC
  CTTTGCTGTGGTCATCATGGTCACCGTCCTTGGAGTGGTGTTTGGAAAAAATGACGTATGGTTCTGGGTC
  ATCTTCTCTGCAATCCACGTTCTGGCCTCGCTAGCCCTCAGCACCCAGATATATTATATGGGTCGTTTCA
  AGATAGATTTGGGAATTTTCCGGCGGGCTGCCATGGTGTTCTACACAGACTGTATCCAGCAGTGTAGCCG
  ACCTCTATATATGGATAGAATGGTGTTGCTGGTTGTGGGGAATCTGGTTAACTGGTCCTTCGCCCTCTTT
  GGATTGATATACCGCCCCAGGGACTTTGCTTCCTACATGCTGGGCATCTTCATCTGTAACCTTTTGCTGT
  ACCTGGCCTTTTACATCATCATGAAGCTCCGCAGCTCTGAAAAGGTCCTCCCAGTCCCGCTCTTCTGCAT
  CGTGGCCACCGCTGTGATGTGGGCTGCCGCCCTATATTTTTTCTTCCAGAATCTCAGCAGCTGGGAGGGA
  ACTCCGGCCGAATCCCGGGAGAAGAACCGCGAGTGCATTCTGCTGGATTTCTTCGATGACCATGACATCT
  GGCACTTCCTCTCTGCTACTGCTCTGTTTTTCTCATTCTTGGTTTTGTTAACTTTGGATGATGACCTTGA
  TGTGGTTCGGAGAGACCAGATCCCTGTCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224950 representing NM_017699
  Red=Cloning site Green=Tags(s)
  
  MRGCLRLALLCALPWLLLAASPGHPAKSPRQPPAPRRDPFDAARGADFDHVYSGVVNLSTENIYSFNYTS
  QPDQVTAVRVYVNSSSENLNYPVLVVVRQQKEVLSWQVPLLFQGLYQRSYNYQEVSRTLCPSEATNETGP
  LQQLIFVDVASMAPLGAQYKLLVTKLKHFQLRTNVAFHFTASPSQPQYFLYKFPKDVDSVIIKVVSEMAY
  PCSVVSVQNIMCPVYDLDHNVEFNGVYQSMTKKAAITLQKKDFPGEQFFVVFVIKPEDYACGGSFFIQEK
  ENQTWNLQRKKNLEVTIVPSIKESVYVKSSLFSVFIFLSFYLGCLLVGFVHYLRFQRKSIDGSFGSNDGS
  GNMVASHPIAASTPEGSNYGTIDESSSSPGRQMSSSDGGPPGQSDTDSSVEESDFDTMPDIESDKNIIRT
  KMFLYLSDLSRKDRRIVSKKYKIYFWNIITIAVFYALPVIQLVITYQTVVNVTGNQDICYYNFLCAHPLG
  VLSAFNNILSNLGHVLLGFLFLLIVLRRDILHRRALEAKDIFAVEYGIPKHFGLFYAMGIALMMEGVLSA
  CYHVCPNYSNFQFDTSFMYMIAGLCMLKLYQTRHPDINASAYSAYASFAVVIMVTVLGVVFGKNDVWFWV
  IFSAIHVLASLALSTQIYYMGRFKIDLGIFRRAAMVFYTDCIQQCSRPLYMDRMVLLVVGNLVNWSFALF
  GLIYRPRDFASYMLGIFICNLLLYLAFYIIMKLRSSEKVLPVPLFCIVATAVMWAAALYFFFQNLSSWEG
  TPAESREKNRECILLDFFDDHDIWHFLSATALFFSFLVLLTLDDDLDVVRRDQIPVF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017699
• ORF Size: 2481 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017699.3
• RefSeq Size: 5021 bp
• RefSeq ORF: 2484 bp
• Locus ID: 54847
• UniProt ID: Q9NXL6
• Cytogenetics: 3q13.2
• Protein Families: Transmembrane
• MW: 93.7 kDa
• Gene Summary: The protein encoded by this gene belongs to SID1 family of transmembrane dsRNA-gated channels. Family members transport dsRNA into cells and are required for systemic RNA interference. [provided by RefSeq, May 2017]