ABCB8 (NM_007188) Human Tagged ORF Clone

CAT#: RC224948

ABCB8 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 8 (ABCB8), nuclear gene encoding mitochondrial protein

Product Images

Western blot validation of overexpression lysate (Cat# LY416139) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224948 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABCB8
• Synonyms: EST328128; M-ABC1; MABC1; MITOSUR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224948 representing NM_007188
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGTGCATTTATTTCGGGTCGGGATTCGGGGTGGCCCATTCCCAGGCAGGCTGCTACCGCCCCTCC
  GCTTCCAGACATTCTCAGCTGTCAGGTACTCTGATGGCTACCGCAGCTCCTCCCTCCTCCGGGCCGTGGC
  CCACCTGCGGTCCCAGCTCTGGGCCCACCTCCCTCGAGCCCCCCTAGCTCCCAGATGGAGCCCCTCTGCC
  TGGTGCTGGGTTGGGGGAGCCCTGCTAGGCCCCATGGTACTGAGTAAGCATCCCCACCTCTGCCTTGTGG
  CCCTGTGTGAGGCAGAAGAGGCCCCTCCTGCCAGCTCCACACCCCATGTCGTGGGGTCTCGCTTTAACTG
  GAAGCTCTTCTGGCAGTTTCTGCACCCCCACCTGCTGGTCCTGGGGGTAGCCGTCGTGCTGGCCTTGGGT
  GCGGCACTCGTGAATGTACAGATCCCCCTGCTCCTGGGCCAGCTGGTAAAGGTCGTGGCCAAGTACACAA
  GGGACCACGTAGGGAGTTTCATGACTGAGTCCCAGAATCTCAGCACCCACCTGCTTATCCTCTATGGTGT
  CCAGGGACTGCTGACCTTCGGGTACCTGGTGCTGCTGTCCCACGTTGGCGAGCGCATGGCTGTGGACATG
  CGGAGGGCCCTCTTCAGCTCCCTGCTCCGACAAAACATCACCTTCTTTGACGCCAATAAGACAGGGCAGC
  TGGTGAGCCGCTTGACAACTGACGTGCAGGAATTTAAGTCATCCTTCAAGCTTGTCATCTCCCAGGGGCT
  GCGAAGCTGCTCCCAGGTGGCAGGCTGCCTGGTGTCCCTGTCCATGCTGTCGACACGCCTCACGCTGCTG
  CTGATGGTGGCCACACCAGCCCTGATGGGAGTGGGCACCCTGATGGGCTCAGGCCTCCGAAAATTGTCTC
  GCCAGTGTCAGGAACATATCGCCAGGGCAATGGGCGTAGCAGACGAGGCCCTGGGCAATGTGCGGACTGT
  GCGAGCCTTGGCCATGGAGCAACGGGAAGAGGAGCGCTATGGGGCAGAGCTGGAAGCCTGCCGCTGCCGG
  GCAGAGGAGCTGGGCCGCGGCATCGCCTTGTTCCAAGGGCTTTCCAACATCGCCTTCAACTGCATGGTCT
  TGGGTACCCTATTTATTGGGGGCTCCCTTGTGGCCGGACAGCAGCTGACAGGGGGAGACCTCATGTCCTT
  CCTGGTGGCCTCCCAGACAGTGCAAAGGTCCATGGCCAACCTCTCTGTCCTGTTTGGGCAGGTGGTCCGG
  GGGCTGAGTGCAGGTGCCCGGGTCTTTGAGTACATGGCCCTGAACCCCTGCATCCCACTGTCTGGGGGCT
  GCTGCGTCCCCAAAGAGCAGCTGCGTGGCTCCGTTACATTTCAGAACGTCTGCTTCAGCTACCCCTGCCG
  CCCCGGCTTCGAGGTGCTGAAAGACTTCACCCTGACGCTGCCCCCTGGCAAGATCGTGGCCCTCGTGGGC
  CAGTCTGGCGGAGGAAAGACCACCGTGGCTTCCCTGCTGGAGCGCTTCTACGACCCCACGGCAGGCGTGG
  TGATGCTGGATGGGCGGGACCTGCGCACCCTTGACCCCTCCTGGCTCCGGGGCCAGGTTGTCGGTTTCAT
  CAGCCAGGAGCCCGTCCTGTTTGGGACGACCATCATGGAAAACATCCGCTTTGGGAAGCTGGAAGCTTCC
  GATGAAGAGGTGTACACAGCCGCCCGGGAAGCGAATGCTCACGAGTTCATCACCAGTTTCCCCGAGGGCT
  ACAACACGGTCGTCGGTGAACGGGGCACTACCCTGTCTGGGGGCCAGAAGCAGCGCCTGGCCATCGCCCG
  AGCCCTTATCAAGCAGCCCACGGTGCTGATACTGGATGAAGCTACCAGCGCGCTGGATGCAGAGTCCGAG
  CGGGTTGTACAGGAGGCCCTGGACCGGGCCAGTGCAGGCCGCACGGTGCTGGTAATTGCCCACCGGCTCA
  GCACTGTCCGTGGGGCCCACTGCATTGTCGTCATGGCCGATGGCCGTGTCTGGGAGGCTGGGACACATGA
  AGAGCTCCTGAAGAAAGGCGGGCTATACGCCGAGCTCATCCGGAGGCAGGCCCTGGATGCCCCGAGGACA
  GCGGCCCCACCGCCCAAAAAGCCAGAAGGCCCCAGGAGCCACCAGCACAAGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224948 representing NM_007188
  Red=Cloning site Green=Tags(s)
  
  MLVHLFRVGIRGGPFPGRLLPPLRFQTFSAVRYSDGYRSSSLLRAVAHLRSQLWAHLPRAPLAPRWSPSA
  WCWVGGALLGPMVLSKHPHLCLVALCEAEEAPPASSTPHVVGSRFNWKLFWQFLHPHLLVLGVAVVLALG
  AALVNVQIPLLLGQLVKVVAKYTRDHVGSFMTESQNLSTHLLILYGVQGLLTFGYLVLLSHVGERMAVDM
  RRALFSSLLRQNITFFDANKTGQLVSRLTTDVQEFKSSFKLVISQGLRSCSQVAGCLVSLSMLSTRLTLL
  LMVATPALMGVGTLMGSGLRKLSRQCQEHIARAMGVADEALGNVRTVRALAMEQREEERYGAELEACRCR
  AEELGRGIALFQGLSNIAFNCMVLGTLFIGGSLVAGQQLTGGDLMSFLVASQTVQRSMANLSVLFGQVVR
  GLSAGARVFEYMALNPCIPLSGGCCVPKEQLRGSVTFQNVCFSYPCRPGFEVLKDFTLTLPPGKIVALVG
  QSGGGKTTVASLLERFYDPTAGVVMLDGRDLRTLDPSWLRGQVVGFISQEPVLFGTTIMENIRFGKLEAS
  DEEVYTAAREANAHEFITSFPEGYNTVVGERGTTLSGGQKQRLAIARALIKQPTVLILDEATSALDAESE
  RVVQEALDRASAGRTVLVIAHRLSTVRGAHCIVVMADGRVWEAGTHEELLKKGGLYAELIRRQALDAPRT
  AAPPPKKPEGPRSHQHKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007188
• ORF Size: 2154 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007188.2, NP_009119.1
• RefSeq Size: 2439 bp
• RefSeq ORF: 2157 bp
• Locus ID: 11194
• UniProt ID: Q9NUT2
• Cytogenetics: 7q36.1
• Domains: ABC_membrane, ABC_tran, AAA
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• MW: 77.9 kDa
• Gene Summary: This nuclear gene encodes a multi-pass membrane protein that is targeted to the mitochondrial inner membrane. The encoded protein is an ATP-dependent transporter that may mediate the passage of organic and inorganic molecules out of the mitochondria. Loss of function of the related gene in mouse results in a disruption of iron homeostasis between the mitochondria and cytosol. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]