FAM105B (OTULIN) (NM_138348) Human Tagged ORF Clone

CAT#: RC224840

FAM105B (Myc-DDK-tagged)-Human family with sequence similarity 105, member B (FAM105B)

Product Images

Circular map for RC224840

• 

  Western blot validation of overexpression lysate (Cat# LY408613) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224840 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAM105B
• Synonyms: AIPDS; FAM105B; GUM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224840 representing NM_138348
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCGGGGGACTATGCCCCAGCCCGAAGCGTGGCCAGGCGCGAGCTGCGCCGAGACGCCGGCGCGGG
  AGGCGGCGGCCACGGCGCGGGACGGCGGGAAGGCGGCGGCCAGCGGGCAGCCGCGGCCCGAGATGCAGTG
  CCCGGCCGAGCATGAGGAGGACATGTACCGTGCTGCAGATGAAATAGAAAAGGAGAAAGAATTGCTTATA
  CATGAAAGAGGGGCATCAGAACCGAGATTAAGCGTAGCTCCTGAAATGGATATCATGGACTACTGCAAAA
  AAGAATGGAGAGGAAATACACAGAAAGCAACGTGTATGAAAATGGGCTATGAAGAGGTTTCTCAGAAGTT
  CACCTCCATACGGCGAGTCCGTGGTGATAATTACTGTGCACTGAGGGCCACGCTGTTCCAGGCCATGAGC
  CAGGCTGTGGGGCTGCCGCCCTGGCTGCAGGACCCGGAGCTCATGCTGTTACCAGAAAAACTCATAAGCA
  AATACAACTGGATCAAGCAATGGAAACTTGGACTGAAATTTGATGGGAAGAATGAGGACCTGGTTGATAA
  AATTAAAGAGTCCCTTACTCTGCTGAGGAAGAAGTGGGCAGGCTTGGCTGAAATGAGAACTGCTGAAGCA
  AGACAGATAGCTTGTGATGAACTATTCACAAATGAGGCGGAGGAATATAGCCTCTATGAAGCTGTAAAAT
  TTCTAATGCTAAACAGAGCCATTGAACTATATAATGATAAAGAGAAAGGAAAGGAAGTACCATTTTTCTC
  TGTGCTTCTGTTTGCTCGGGACACATCAAATGACCCAGGACAGCTTCTGAGGAACCACCTCAACCAGGTG
  GGACACACTGGTGGTCTTGAACAGGTTGAAATGTTCCTTCTTGCCTATGCTGTGCGCCACACCATCCAGG
  TGTACCGGCTCTCCAAGTACAACACGGAAGAATTCATCACAGTCTACCCCACCGACCCACCCAAGGACTG
  GCCAGTGGTAACGCTCATTGCTGAGGACGATCGGCACTATAACATCCCCGTCAGAGTGTGTGAGGAGACC
  AGTCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224840 representing NM_138348
  Red=Cloning site Green=Tags(s)
  
  MSRGTMPQPEAWPGASCAETPAREAAATARDGGKAAASGQPRPEMQCPAEHEEDMYRAADEIEKEKELLI
  HERGASEPRLSVAPEMDIMDYCKKEWRGNTQKATCMKMGYEEVSQKFTSIRRVRGDNYCALRATLFQAMS
  QAVGLPPWLQDPELMLLPEKLISKYNWIKQWKLGLKFDGKNEDLVDKIKESLTLLRKKWAGLAEMRTAEA
  RQIACDELFTNEAEEYSLYEAVKFLMLNRAIELYNDKEKGKEVPFFSVLLFARDTSNDPGQLLRNHLNQV
  GHTGGLEQVEMFLLAYAVRHTIQVYRLSKYNTEEFITVYPTDPPKDWPVVTLIAEDDRHYNIPVRVCEET
  SL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138348
• ORF Size: 1056 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138348.6
• RefSeq Size: 7896 bp
• RefSeq ORF: 1059 bp
• Locus ID: 90268
• UniProt ID: Q96BN8
• Cytogenetics: 5p15.2
• MW: 40.1 kDa
• Gene Summary: This gene encodes a member of the peptidase C65 family of ubiquitin isopeptidases. Members of this family remove ubiquitin from proteins. The encoded enzyme specifically recognizes and removes M1(Met1)-linked, or linear, ubiquitin chains from protein substrates. Linear ubiquitin chains are known to regulate the NF-kappa B signaling pathway in the context of immunity and inflammation. Mutations in this gene cause a potentially fatal autoinflammatory syndrome in human patients. [provided by RefSeq, Sep 2016]