FAM105B (OTULIN) (NM_138348) Human Tagged ORF Clone
FAM105B (Myc-DDK-tagged)-Human family with sequence similarity 105, member B (FAM105B)
|Type||Human Tagged ORF Clone|
|Synonyms||AIPDS; FAM105B; GUM|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC224840 representing NM_138348
Red=Cloning site Blue=ORF Green=Tags(s)
>RC224840 representing NM_138348
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1056 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||7896 bp|
|RefSeq ORF||1059 bp|
|Gene Summary||This gene encodes a member of the peptidase C65 family of ubiquitin isopeptidases. Members of this family remove ubiquitin from proteins. The encoded enzyme specifically recognizes and removes M1(Met1)-linked, or linear, ubiquitin chains from protein substrates. Linear ubiquitin chains are known to regulate the NF-kappa B signaling pathway in the context of immunity and inflammation. Mutations in this gene cause a potentially fatal autoinflammatory syndrome in human patients. [provided by RefSeq, Sep 2016]|
|cDNA Clone Resources|
|RC224840L1||Lenti ORF clone of Human family with sequence similarity 105, member B (FAM105B), Myc-DDK-tagged||
|RC224840L2||Lenti ORF clone of Human family with sequence similarity 105, member B (FAM105B), mGFP tagged||
|RC224840L3||Lenti ORF clone of Human family with sequence similarity 105, member B (FAM105B), Myc-DDK-tagged||
|RC224840L4||Lenti ORF clone of Human family with sequence similarity 105, member B (FAM105B), mGFP tagged||
|RG224840||FAM105B (GFP-tagged) - Human family with sequence similarity 105, member B (FAM105B)||
|SC100495||FAM105B (untagged)-Human family with sequence similarity 105, member B (FAM105B)||
|SC317556||FAM105B (untagged)-Human family with sequence similarity 105, member B (FAM105B)||