GAS1 (NM_002048) Human Tagged ORF Clone

CAT#: RC224804

GAS1 (Myc-DDK-tagged)-Human growth arrest-specific 1 (GAS1)

Product Images

Circular map for RC224804

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  Western blot validation of overexpression lysate (Cat# LY419569) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224804 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GAS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224804 representing NM_002048
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCCGCGCTGCTGGGCGGCGGCGGCGAGGCCCGCGGGGGGACAGTGCCGGGCGCCTGGCTGTGCC
  TGATGGCGCTGCTGCAGCTGCTGGGCTCGGCGCCGCGGGGATCGGGGCTGGCGCACGGCCGCCGCCTCAT
  CTGCTGGCAGGCGCTGCTGCAGTGCCAGGGGGAGCCGGAGTGCAGCTACGCCTACAACCAGTACGCCGAG
  GCGTGCGCGCCGGTGCTGGCGCAGCACGGCGGGGGCGACGCGCCCGGGGCCGCCGCCGCCGCTTTCCCGG
  CCTCGGCCGCCTCTTTCTCGTCGCGCTGGCGCTGCCCGAGTCACTGCATCTCGGCCCTCATTCAGCTCAA
  CCACACGCGCCGCGGGCCCGCCCTGGAGGACTGTGACTGCGCGCAGGACGAGAACTGCAAGTCCACCAAG
  CGCGCCATTGAGCCGTGCCTGCCCCGGACGAGCGGCGGCGGCGCGGGCGGCCCCGGCGCGGGCGGGGTCA
  TGGGCTGCACCGAGGCCCGGCGGCGCTGCGACCGCGACAGCCGCTGCAACCTGGCGCTGAGCCGCTACCT
  GACCTACTGCGGCAAAGTCTTCAACGGGCTGCGCTGCACGGACGAATGCCGCACCGTCATTGAGGACATG
  CTGGCTATGCCCAAGGCGGCGCTGCTCAACGACTGCGTGTGCGACGGCCTCGAGCGGCCCATCTGCGAGT
  CGGTCAAGGAGAACATGGCCCGCCTGTGCTTCGGCGCCGAGCTGGGCAACGGCCCCGGCAGCAGCGGCTC
  GGACGGGGGCCTGGACGACTACTACGATGAGGACTACGATGACGAGCAGCGCACCGGGGGCGCGGGTGGT
  GAGCAGCCGCTGGACGACGACGACGGCGTCCCGCACCCACCGCGCCCGGGCAGCGGCGCTGCTGCATCGG
  GCGGCCGCGGGGACCTGCCCTATGGGCCTGGGCGCAGGAGCAGCGGCGGCGGCGGCCGCTTGGCGCCCCG
  GGGCGCCTGGACCCCACTCGCCTCCATCTTGCTGCTGCTGCTTGGGCCGCTCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224804 representing NM_002048
  Red=Cloning site Green=Tags(s)
  
  MVAALLGGGGEARGGTVPGAWLCLMALLQLLGSAPRGSGLAHGRRLICWQALLQCQGEPECSYAYNQYAE
  ACAPVLAQHGGGDAPGAAAAAFPASAASFSSRWRCPSHCISALIQLNHTRRGPALEDCDCAQDENCKSTK
  RAIEPCLPRTSGGGAGGPGAGGVMGCTEARRRCDRDSRCNLALSRYLTYCGKVFNGLRCTDECRTVIEDM
  LAMPKAALLNDCVCDGLERPICESVKENMARLCFGAELGNGPGSSGSDGGLDDYYDEDYDDEQRTGGAGG
  EQPLDDDDGVPHPPRPGSGAAASGGRGDLPYGPGRRSSGGGGRLAPRGAWTPLASILLLLLGPLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002048
• ORF Size: 1035 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002048.3
• RefSeq Size: 2828 bp
• RefSeq ORF: 1038 bp
• Locus ID: 2619
• UniProt ID: P54826
• Cytogenetics: 9q21.33
• Protein Pathways: Hedgehog signaling pathway
• MW: 35.5 kDa
• Gene Summary: Growth arrest-specific 1 plays a role in growth suppression. GAS1 blocks entry to S phase and prevents cycling of normal and transformed cells. Gas1 is a putative tumor suppressor gene. [provided by RefSeq, Jul 2008]