GDF10 (NM_004962) Human Tagged ORF Clone

CAT#: RC224791

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GDF10 (Myc-DDK-tagged)-Human growth differentiation factor 10 (GDF10)



  "NM_004962" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 511.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol GDF10
Synonyms BIP; BMP-3b; BMP3B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC224791 representing NM_004962
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCATGTCCCCGCTCGGACCAGCCCGGGACCCGGGCCCCAGCTGCTGCTGCTGCTGCTGCCGTTGT
TTCTGCTGTTGCTCCGGGATGTGGCCGGCAGCCACAGGGCCCCCGCCTGGTCCGCACTGCCCGCGGCCGC
CGACGGCCTGCAGGGGGACAGGGATCTCCAGCGGCACCCTGGGGACGCGGCCGCCACGTTGGGCCCCAGC
GCCCAGGACATGGTCGCTGTCCACATGCACAGGCTCTATGAGAAGTACAGCCGGCAGGGCGCGCGGCCGG
GAGGGGGCAACACGGTCCGCAGCTTCAGGGCCAGGCTGGAAGTGGTCGACCAGAAGGCCGTGTATTTCTT
CAACCTGACTTCCATGCAAGACTCGGAAATGATCCTTACGGCCACTTTCCACTTCTACTCAGAGCCGCCT
CGGTGGCCTCGAGCGCTCGAGGTGCTATGCAAGCCGCGGGCCAAGAACGCTTCAGGCCGCCCGCTGCCCC
TGGGCCCGCCCACACGCCAGCACCTGCTCTTCCGCAGCCTCTCGCAGAACACGGCCACACAGGGGCTACT
CCGCGGGGCCATGGCCCTGGCGCCCCCACCGCGCGGCCTGTGGCAGGCCAAGGACATCTCCCCCATCGTC
AAGGCGGCCCGCCGGGATGGCGAGCTGCTCCTCTCCGCCCAGCTGGATTCTGAGGAGAGGGACCCGGGGG
TGCCCCGGCCCAGCCCCTATGCGCCCTACATCCTAGTCTATGCCAACGATCTGGCCATCTCGGAGCCCAA
CAGCGTGGCAGTGACGCTGCAGAGATACGACCCCTTCCCTGCCGGAGACCCCGAGCCCCGCGCAGCCCCC
AACAACTCAGCGGACCCCCGCGTGCGCCGAGCCGCGCAGGCCACTGGGCCCCTCCAGGACAACGAGCTGC
CGGGGCTGGATGAGAGGCCGCCGCGCGCCCACGCACAGCACTTCCACAAGCACCAGCTGTGGCCCAGCCC
CTTCCGGGCGCTGAAACCCCGGCCAGGGCGCAAAGACCGCAGGAAGAAGGGCCAGGAGGTGTTCATGGCC
GCCTCGCAGGTGCTGGACTTTGACGAGAAGACGATGCAGAAAGCCCGGAGGAAGCAGTGGGATGAGCCGA
GGGTGTGCTCCCGGAGGTACCTGAAGGTGGACTTCGCAGACATCGGCTGGAATGAATGGATAATCTCACC
GAAATCTTTTGATGCCTACTACTGCGCGGGAGCATGTGAGTTCCCCATGCCTAAGATCGTTCGTCCATCC
AACCATGCCACCATCCAGAGCATTGTCAGGGCTGTGGGCATCATCCCTGGCATCCCAGAGCCCTGCTGTG
TTCCCGATAAGATGAACTCCCTTGGGGTCCTCTTCCTGGATGAGAATCGGAATGTGGTTCTGAAGGTGTA
CCCCAACATGTCCGTGGACACCTGTGCCTGCCGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC224791 representing NM_004962
Red=Cloning site Green=Tags(s)

MAHVPARTSPGPGPQLLLLLLPLFLLLLRDVAGSHRAPAWSALPAAADGLQGDRDLQRHPGDAAATLGPS
AQDMVAVHMHRLYEKYSRQGARPGGGNTVRSFRARLEVVDQKAVYFFNLTSMQDSEMILTATFHFYSEPP
RWPRALEVLCKPRAKNASGRPLPLGPPTRQHLLFRSLSQNTATQGLLRGAMALAPPPRGLWQAKDISPIV
KAARRDGELLLSAQLDSEERDPGVPRPSPYAPYILVYANDLAISEPNSVAVTLQRYDPFPAGDPEPRAAP
NNSADPRVRRAAQATGPLQDNELPGLDERPPRAHAQHFHKHQLWPSPFRALKPRPGRKDRRKKGQEVFMA
ASQVLDFDEKTMQKARRKQWDEPRVCSRRYLKVDFADIGWNEWIISPKSFDAYYCAGACEFPMPKIVRPS
NHATIQSIVRAVGIIPGIPEPCCVPDKMNSLGVLFLDENRNVVLKVYPNMSVDTCACR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004962
ORF Size 1434 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004962.5
RefSeq Size 2674 bp
RefSeq ORF 1437 bp
Locus ID 2662
UniProt ID P55107
Cytogenetics 10q11.22
Protein Families Druggable Genome, Secreted Protein
MW 52.9 kDa
Gene Summary This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This promotes neural repair after stroke. Additionally, this protein may act as a tumor suppressor and reduced expression of this gene is associated with oral cancer. [provided by RefSeq, Jul 2016]

Other Versions

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