Neurogenin3 (NEUROG3) (NM_020999) Human Tagged ORF Clone

CAT#: RC224767

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NEUROG3 (Myc-DDK-tagged)-Human neurogenin 3 (NEUROG3)



Reconstitution Protocol

USD 450.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol NEUROG3
Synonyms Atoh5; bHLHa7; Math4B; NGN-3; ngn3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC224767 representing NM_020999
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGCCTCAACCCTCGGGTGCGCCCACTGTCCAAGTGACCCGTGAGACGGAGCGGTCCTTCCCCAGAG
CCTCGGAAGACGAAGTGACCTGCCCCACGTCCGCCCCGCCCAGCCCCACTCGCACACGGGGGAACTGCGC
AGAGGCGGAAGAGGGAGGCTGCCGAGGGGCCCCGAGGAAGCTCCGGGCACGGCGCGGGGGACGCAGCCGG
CCTAAGAGCGAGTTGGCACTGAGCAAGCAGCGACGGAGTCGGCGAAAGAAGGCCAACGACCGCGAGCGCA
ATCGAATGCACAACCTCAACTCGGCACTGGACGCCCTGCGCGGTGTCCTGCCCACCTTCCCAGACGACGC
GAAGCTCACCAAGATCGAGACGCTGCGCTTCGCCCACAACTACATCTGGGCGCTGACTCAAACGCTGCGC
ATAGCGGACCACAGCTTGTACGCGCTGGAGCCGCCGGCGCCGCACTGCGGGGAGCTGGGCAGCCCAGGCG
GTTCCCCCGGGGACTGGGGGTCCCTCTACTCCCCAGTCTCCCAGGCTGGCAGCCTGAGTCCCGCCGCGTC
GCTGGAGGAGCGACCCGGGCTGCTGGGGGCCACCTCTTCCGCCTGCTTGAGCCCAGGCAGTCTGGCTTTC
TCAGATTTTCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC224767 representing NM_020999
Red=Cloning site Green=Tags(s)

MTPQPSGAPTVQVTRETERSFPRASEDEVTCPTSAPPSPTRTRGNCAEAEEGGCRGAPRKLRARRGGRSR
PKSELALSKQRRSRRKKANDRERNRMHNLNSALDALRGVLPTFPDDAKLTKIETLRFAHNYIWALTQTLR
IADHSLYALEPPAPHCGELGSPGGSPGDWGSLYSPVSQAGSLSPAASLEERPGLLGATSSACLSPGSLAF
SDFL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_020999
ORF Size 642 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_020999.4
RefSeq Size 1167 bp
RefSeq ORF 645 bp
Locus ID 50674
UniProt ID Q9Y4Z2
Cytogenetics 10q22.1
Protein Families ES Cell Differentiation/IPS
Protein Pathways Maturity onset diabetes of the young
MW 22.9 kDa
Gene Summary The protein encoded by this gene is a basic helix-loop-helix (bHLH) transcription factor involved in neurogenesis. The encoded protein likely acts as a heterodimer with another bHLH protein. Defects in this gene are a cause of congenital malabsorptive diarrhea 4 (DIAR4).[provided by RefSeq, May 2010]
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