BAI2 (ADGRB2) (NM_001703) Human Tagged ORF Clone

CAT#: RC224664

BAI2 (Myc-DDK-tagged)-Human brain-specific angiogenesis inhibitor 2 (BAI2)

Product Images

Circular map for RC224664

• 

  Western blot validation of overexpression lysate (Cat# LY419803) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224664 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BAI2
• Synonyms: brain-specific angiogenesis inhibitor 2; Brain-specific angiongenesis inhibitor-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224664 representing NM_001703
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAATACAGGTTGGATGGGCAAGGGACATAGGATGACCCCAGCCTGTCCCCTCTTACTGTCTGTGA
  TTCTGTCCCTGCGCCTGGCCACCGCCTTCGACCCCGCCCCCAGTGCCTGCTCTGCCCTGGCCTCGGGTGT
  GCTCTACGGGGCCTTCTCGCTGCAGGACCTCTTTCCTACCATCGCCTCGGGCTGCTCCTGGACCCTGGAG
  AACCCTGACCCCACCAAGTACTCCCTCTACCTGCGCTTCAACCGCCAGGAGCAGGTGTGCGCACACTTTG
  CCCCCCGCCTGCTGCCCCTGGACCACTACCTGGTCAACTTTACCTGCCTGCGGCCTAGCCCCGAGGAGGC
  GGTGGCCCAGGCGGAGTCAGAGGTGGGGCGGCCAGAAGAGGAGGAGGCAGAGGCGGCAGCGGGGTTGGAG
  CTGTGCAGCGGCTCAGGCCCCTTTACCTTCCTGCACTTCGACAAGAACTTCGTGCAGCTGTGCCTGTCGG
  CTGAGCCCTCCGAGGCCCCGCGCCTGCTGGCGCCCGCTGCCCTAGCCTTCCGCTTTGTCGAGGTCTTGCT
  CATCAACAACAACAACTCTAGCCAATTCACCTGTGGTGTGCTCTGCCGCTGGAGTGAGGAGTGTGGCCGC
  GCTGCCGGCAGGGCCTGCGGCTTTGCTCAGCCAGGCTGCAGCTGCCCTGGAGAGGCGGGGGCCGGCTCCA
  CCACCACCACATCTCCAGGCCCTCCTGCTGCCCACACCCTGTCCAATGCCCTGGTGCCCGGGGGCCCAGC
  CCCACCTGCTGAGGCCGATTTGCACTCGGGGAGCAGCAATGATCTGTTCACAACCGAGATGAGATATGGT
  GAGGAGCCGGAAGAGGAACCGAAAGTGAAAACCCAGTGGCCGAGGTCTGCAGATGAGCCTGGGCTATACA
  TGGCGCAGACAGGCGACCCGGCGGCTGAGGAGTGGTCCCCGTGGAGCGTGTGTTCCCTGACGTGTGGGCA
  GGGTCTGCAGGTGCGGACCCGCTCCTGTGTGTCCTCCCCCTATGGGACCCTGTGCAGCGGGCCCCTGCGG
  GAGACCAGGCCCTGCAACAATTCAGCCACCTGCCCAGTGCACGGCGTGTGGGAGGAGTGGGGGTCCTGGA
  GCCTGTGCTCCCGCAGCTGCGGGCGGGGGTCCCGGAGCCGGATGCGGACCTGCGTGCCCCCCCAGCACGG
  CGGCAAGGCCTGCGAGGGTCCTGAGCTGCAGACTAAGCTCTGCAGTATGGCTGCCTGCCCGGTGGAAGGC
  CAGTGGTTAGAATGGGGTCCCTGGGGCCCATGCTCCACGTCCTGTGCCAATGGGACCCAACAGCGCAGCC
  GGAAGTGCAGCGTGGCGGGCCCAGCCTGGGCCACATGCACGGGTGCCCTCACTGACACCCGGGAGTGCAG
  CAACCTCGAGTGCCCGGCCACTGATAGCAAGTGGGGGCCATGGAATGCGTGGAGCCTGTGCTCTAAGACG
  TGTGACACAGGCTGGCAGCGCCGCTTCCGCATGTGCCAGGCCACGGGCACGCAGGGCTACCCCTGCGAGG
  GCACCGGAGAGGAGGTGAAGCCTTGTAGTGAGAAGAGGTGTCCAGCCTTCCATGAGATGTGCAGGGATGA
  GTACGTGATGCTGATGACGTGGAAGAAGGCAGCTGCTGGCGAGATCATCTACAACAAGTGCCCCCCGAAT
  GCCTCAGGGTCTGCCAGCCGCCGCTGTCTCCTCAGTGCCCAAGGCGTGGCGTACTGGGGGCTGCCCAGCT
  TTGCTCGCTGCATCTCCCATGAGTACCGCTACCTGTATCTGTCACTTAGGGAGCACCTGGCCAAGGGGCA
  GCGCATGCTGGCAGGCGAGGGCATGTCGCAGGTGGTGCGCAGCCTGCAGGAGCTACTGGCCCGGCGCACC
  TACTATAGTGGGGACCTGCTCTTCTCTGTGGACATTCTGAGGAATGTCACTGACACCTTTAAGAGGGCCA
  CCTACGTGCCCTCGGCTGATGATGTGCAGCGCTTCTTCCAGGTGGTGAGCTTCATGGTGGATGCGGAAAA
  CAAGGAGAAGTGGGACGATGCTCAGCAGGTGTCCCCTGGCTCTGTGCACCTGCTCCGTGTCGTGGAGGAC
  TTCATTCACCTGGTGGGCGATGCTCTCAAGGCCTTCCAGAGCTCTCTGATTGTCACAGATAATCTAGTGA
  TCAGCATTCAGCGAGAGCCCGTCTCAGCTGTGTCCAGTGACATCACGTTCCCCATGCGGGGCCGCCGGGG
  CATGAAGGACTGGGTGCGGCACTCAGAGGACCGCCTCTTCCTGCCCAAGGAGGTGCTCAGCCTCTCCTCC
  CCAGGGAAGCCAGCCACATCTGGGGCAGCAGGCAGCCCTGGCAGGGGGAGGGGCCCAGGAACGGTGCCTC
  CTGGCCCAGGCCACTCCCACCAGCGCCTCCTCCCAGCAGACCCTGATGAGTCCTCCTACTTTGTGATCGG
  TGCTGTACTCTACCGCACCCTTGGCCTCATCCTGCCGCCTCCCAGGCCCCCGCTGGCCGTCACATCCCGG
  GTGATGACAGTGACTGTGCGCCCCCCTACCCAGCCTCCAGCTGAGCCCCTCATCACTGTGGAGCTCTCCT
  ACATCATCAATGGGACCACGGATCCCCATTGCGCCAGCTGGGACTACTCCAGAGCAGATGCCAGCTCAGG
  AGACTGGGACACTGAAAATTGCCAGACCCTGGAGACCCAGGCAGCTCACACCCGCTGCCAGTGCCAGCAC
  CTGTCCACCTTTGCTGTACTAGCCCAGCCGCCCAAGGACCTGACCCTGGAGCTGGCGGGCTCCCCCTCGG
  TCCCCCTGGTGATCGGCTGTGCAGTGTCGTGCATGGCGCTGCTCACCCTGCTCGCCATCTATGCCGCCTT
  TTGGAGGTTCATAAAATCTGAACGCTCCATCATCTTGCTGAACTTCTGCCTGTCCATCTTGGCATCCAAC
  ATCCTGATCCTCGTGGGCCAGTCCCGGGTGCTGAGCAAGGGCGTGTGCACCATGACGGCTGCCTTCCTGC
  ACTTCTTCTTTCTCTCCTCCTTTTGCTGGGTGCTTACCGAGGCCTGGCAGTCCTACCTGGCTGTCATTGG
  GCGGATGCGCACCCGCCTCGTTCGCAAGCGCTTCCTCTGCCTGGGCTGGGGTCTGCCTGCCCTGGTGGTG
  GCCGTGTCTGTTGGCTTTACCCGAACGAAAGGATACGGTACATCCAGCTACTGCTGGCTCTCCCTGGAGG
  GCGGCCTGCTCTACGCCTTTGTGGGCCCTGCAGCCGTCATTGTCCTGGTGAACATGCTCATCGGAATCAT
  CGTCTTCAACAAGCTCATGGCACGTGATGGCATCTCCGACAAATCCAAGAAGCAGAGGGCCGGGTCGGAG
  CGGTGCCCCTGGGCCAGCCTGCTCCTCCCCTGCTCAGCGTGTGGAGCGGTCCCCAGCCCCCTGCTCAGCT
  CAGCCTCGGCCAGGAACGCCATGGCCTCACTCTGGAGCTCCTGCGTGGTGCTGCCCCTGCTGGCGCTCAC
  CTGGATGTCTGCCGTCCTGGCTATGACAGACCGCCGTTCCGTCCTCTTCCAGGCCCTCTTTGCTGTCTTC
  AACTCCGCGCAGGGCTTTGTCATCACTGCTGTGCACTGCTTCCTGCGCCGAGAGGTCCAGGATGTGGTGA
  AGTGCCAGATGGGGGTGTGCCGGGCTGATGAGAGCGAAGACTCCCCTGACTCGTGTAAGAACGGGCAGCT
  GCAGATCCTGTCAGACTTTGAAAAGGATGTGGATCTGGCTTGTCAAACAGTGCTGTTCAAGGAGGTCAAC
  ACTTGCAACCCGTCCACCATCACGGGCACACTATCCCGCCTGTCCCTGGATGAGGATGAGGAGCCCAAGT
  CCTGCCTCGTGGGCCCTGAGGGCAGCCTCAGCTTCTCACCACTGCCTGGGAATATCCTGGTGCCCATGGC
  AGCCTCACCAGGGCTGGGGGAGCCTCCGCCCCCACAGGAGGCCAACCCTGTTTACATGTGTGGGGAGGGT
  GGCCTGCGGCAGCTGGACCTCACATGGCTGCGGCCCACTGAGCCAGGCTCTGAGGGAGACTACATGGTGC
  TGCCCCGGCGGACTTTGAGCCTGCAGCCTGGCGGTGGGGGTGGAGGTGGTGAGGATGCCCCCAGGGCCCG
  GCCGGAGGGGACCCCCCGGCGAGCTGCCAAGACAGTGGCCCACACTGAAGGCTACCCCAGCTTCCTGTCC
  GTGGACCACTCGGGCCTGGGGCTGGGCCCTGCCTATGGATCTCTCCAGAATCCCTATGGAATGACCTTCC
  AACCGCCACCGCCGACACCCAGCGCCCGCCAAGTGCCCGAGCCAGGGGAGCGCAGCCGGACCATGCCTCG
  CACCGTGCCCGGCTCTACCATGAAGATGGGCTCCCTGGAGCGAAAGAAATTACGGTATTCAGACCTGGAC
  TTTGAGAAGGTGATGCACACCCGGAAACGGCATTCAGAACTCTACCACGAGCTCAACCAGAAGTTCCACA
  CTTTCGACCGCTACCGCAGCCAGTCCACGGCCAAGAGGGAGAAGCGGTGGAGTGTGTCCTCGGGTGGGGC
  AGCCGAGCGGAGCGTGTGCACCGATAAGCCCAGCCCTGGGGAGCGCCCCAGCTTGTCCCAACATCGGCGC
  CATCAGAGCTGGAGCACCTTCAAATCTATGACACTGGGCTCGCTGCCCCCCAAGCCCCGAGAACGGCTGA
  CTCTGCACCGGGCAGCAGCCTGGGAGCCCACAGAACCACCGGATGGTGACTTCCAGACAGAGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224664 representing NM_001703
  Red=Cloning site Green=Tags(s)
  
  MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLE
  NPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQAESEVGRPEEEEAEAAAGLE
  LCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGR
  AAGRACGFAQPGCSCPGEAGAGSTTTTSPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYG
  EEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLR
  ETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEG
  QWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGPWNAWSLCSKT
  CDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPN
  ASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRT
  YYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVED
  FIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSS
  PGKPATSGAAGSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSR
  VMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQAAHTRCQCQH
  LSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASN
  ILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVV
  AVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSE
  RCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVF
  NSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVN
  TCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEG
  GLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEGTPRRAAKTVAHTEGYPSFLS
  VDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKMGSLERKKLRYSDLD
  FEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRR
  HQSWSTFKSMTLGSLPPKPRERLTLHRAAAWEPTEPPDGDFQTEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001703
• ORF Size: 4755 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001703.2, NP_001694.2
• RefSeq Size: 5434 bp
• RefSeq ORF: 4758 bp
• Locus ID: 576
• Cytogenetics: 1p35.2
• Protein Families: Druggable Genome, Transmembrane
• MW: 172.5 kDa
• Gene Summary: This gene encodes a a seven-span transmembrane protein that is thought to be a member of the secretin receptor family. The encoded protein is a brain-specific inhibitor of angiogenesis. The mature peptide may be further cleaved into additional products (PMID:20367554). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]