HES3 (NM_001024598) Human Tagged ORF Clone

CAT#: RC224630

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HES3 (Myc-DDK-tagged)-Human hairy and enhancer of split 3 (Drosophila) (HES3)



  "NM_001024598" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 462.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol HES3
Synonyms bHLHb43
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC224630 representing NM_001024598
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAAAAGCGCCGGGCACGCATCAATGTGTCACTGGAGCAGCTCAAGTCGCTGCTGGAGAAACACT
ACTCGCACCAGATCCGGAAGCGCAAATTGGAGAAGGCCGACATCCTGGAGTTGAGCGTGAAGTACATGAG
AAGCCTTCAGAACTCCTTGCAAGGGCTCTGGCCTGTGCCCAGGGGAGCCGAGCAACCGTCGGGCTTCCGC
AGCTGCCTGCCCGGCGTGAGCCAGCTCCTTCGGCGCGGAGATGAGGTCGGCAGCGGCCTGCGCTGCCCCC
TGGTGCCCGAGAGCGCCGCCGGCAGCACCATGGACAGCGCCGGGTTGGGCCAGGAGGCGCCCGCGCTGTT
CCGCCCTTGCACCCCTGCCGTCTGGGCTCCTGCTCCGGCCGCCGGCGGCCCGCGGTCCCCACCACCCCTG
CTCCTCCTCCCCGAAAGTCTCCCTGGCTCGTCCGCCAGCGTCCCCCCGCCGCAGCCAGCGTCGAGTCGCT
GCGCCGAGAGTCCCGGGCTGGGCCTGCGCGTGTGGCGGCCCTGGGGAAGCCCCGGGGATGACCTGAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC224630 representing NM_001024598
Red=Cloning site Green=Tags(s)

MEKKRRARINVSLEQLKSLLEKHYSHQIRKRKLEKADILELSVKYMRSLQNSLQGLWPVPRGAEQPSGFR
SCLPGVSQLLRRGDEVGSGLRCPLVPESAAGSTMDSAGLGQEAPALFRPCTPAVWAPAPAAGGPRSPPPL
LLLPESLPGSSASVPPPQPASSRCAESPGLGLRVWRPWGSPGDDLN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001024598
ORF Size 558 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001024598.4
RefSeq Size 1355 bp
RefSeq ORF 561 bp
Locus ID 390992
UniProt ID Q5TGS1
Cytogenetics 1p36.31
MW 19.8 kDa
Gene Summary Transcriptional repressor of genes that require a bHLH protein for their transcription.[UniProtKB/Swiss-Prot Function]

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