DCBLD2 (NM_080927) Human Tagged ORF Clone

CAT#: RC224483

DCBLD2 (Myc-DDK-tagged)-Human discoidin, CUB and LCCL domain containing 2 (DCBLD2)

Product Images

Western blot validation of overexpression lysate (Cat# LY403322) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224483 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DCBLD2
• Synonyms: CLCP1; ESDN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224483 representing NM_080927
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGCCGGGCGGTGGTGAGAGCCAGGCGCTGCCCGCAGTGTCCCCAAGTCCGGGCCGCGGCCGCCG
  CCCCCGCCTGGGCCGCGCTCCCCCTCTCCCGCTCCCTCCCTCCCTGCTCCAACTCCTCCTCCTTCTCCAT
  GCCTCTGTTCCTCCTGCTCTTACTTGTCCTGCTCCTGCTGCTCGAGGACGCTGGAGCCCAGCAAGGTGAT
  GGATGTGGACACACTGTACTAGGCCCTGAGAGTGGAACCCTTACATCCATAAACTACCCACAGACCTATC
  CCAACAGCACTGTTTGTGAATGGGAGATCCGTGTAAAGATGGGAGAGAGAGTTCGCATCAAATTTGGTGA
  CTTTGACATTGAAGATTCTGATTCTTGTCACTTTAATTACTTGAGAATTTATAATGGAATTGGAGTCAGC
  AGAACTGAAATAGGCAAATACTGTGGTCTGGGGTTGCAAATGAACCATTCAATTGAATCAAAAGGCAATG
  AAATCACATTGCTGTTCATGAGTGGAATCCATGTTTCTGGACGCGGATTTTTGGCCTCATACTCTGTTAT
  AGATAAACAAGATCTAATTACTTGTTTGGACACTGCATCCAATTTTTTGGAACCTGAGTTCAGTAAGTAC
  TGCCCAGCTGGTTGTCTGCTTCCTTTTGCTGAGATATCTGGAACAATTCCTCATGGATATAGAGATTCCT
  CGCCATTGTGCATGGCTGGTGTGCATGCAGGAGTAGTGTCAAACACGTTGGGCGGCCAAATCAGTGTTGT
  AATTAGTAAAGGTATTCCCTATTATGAAAGTTCTTTGGCTAACAACGTCACATCTGTGGTGGGACACTTA
  TCTACAAGTCTTTTTACATTTAAGACAAGTGGATGTTATGGAACACTGGGGATGGAGTCTGGTGTGATCG
  CGGATCCTCAAATAACAGCATCATCTGTGCTGGAGTGGACTGACCACACAGGGCAAGAGAACAGTTGGAA
  ACCCAAAAAAGCCAGGCTGAAAAAACCTGGACCGCCTTGGGCTGCTTTTGCCACTGATGAATACCAGTGG
  TTACAAATAGATTTGAATAAGGAAAAGAAAATAACAGGCATTATAACCACTGGATCCACCATGGTGGAGC
  ACAATTACTATGTGTCTGCCTACAGAATCCTGTACAGTGATGATGGGCAGAAATGGACTGTGTACAGAGA
  GCCTGGTGTGGAGCAAGATAAGATATTTCAAGGAAACAAAGATTATCACCAGGATGTGCGTAATAACTTT
  TTGCCACCAATTATTGCACGTTTTATTAGAGTGAATCCTACCCAATGGCAGCAGAAAATTGCCATGAAAA
  TGGAGCTGCTCGGATGTCAGTTTATTCCTAAAGGTCGTCCTCCAAAACTTACTCAACCTCCACCTCCTCG
  GAACAGCAATGACCTCAAAAACACTACAGCCCCTCCAAAAATAGCCAAAGGTCGTGCCCCAAAATTTACG
  CAACCACTACAACCTCGCAGTAGCAATGAATTTCCTGCACAGACAGAACAAACAACTGCCAGTCCTGATA
  TCAGAAATACTACCGTAACTCCAAATGTAACCAAAGATGTAGCGCTGGCTGCAGTTCTTGTCCCTGTGCT
  GGTCATGGTCCTCACTACTCTCATTCTCATATTAGTGTGTGCTTGGCACTGGAGAAACAGAAAGAAAAAA
  ACTGAAGGCACCTATGACTTACCTTACTGGGACCGGGCAGGTTGGTGGAAAGGAATGAAGCAGTTTCTTC
  CTGCAAAAGCAGTGGACCATGAGGAAACCCCAGTTCGCTATAGCAGCAGCGAAGTTAATCACCTGAGTCC
  AAGAGAAGTCACCACAGTGCTGCAGGCTGACTCTGCAGAGTATGCTCAGCCACTGGTAGGAGGAATTGTT
  GGTACACTTCATCAAAGATCTACCTTTAAACCAGAAGAAGGAAAAGAAGCAGGCTATGCAGACCTAGATC
  CTTACAACTCACCAGGGCAGGAAGTTTATCATGCCTATGCTGAACCACTCCCAATTACGGGGCCTGAGTA
  TGCAACCCCAATCATCATGGACATGTCAGGGCACCCCACAACTTCAGTTGGTCAGCCCTCCACATCCACT
  TTCAAGGCTACGGGGAACCAACCTCCCCCACTAGTGGGAACTTACAATACACTTCTCTCCAGGACTGACA
  GCTGCTCCTCAGCCCAGGCCCAGTATGATACCCCGAAAGCTGGGAAGCCAGGTCTACCTGCCCCAGACGA
  ATTGGTGTACCAGGTGCCACAGAGCACACAAGAAGTATCAGGAGCAGGAAGGGATGGGGAATGTGATGTT
  TTTAAAGAAATCCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224483 representing NM_080927
  Red=Cloning site Green=Tags(s)
  
  MASRAVVRARRCPQCPQVRAAAAAPAWAALPLSRSLPPCSNSSSFSMPLFLLLLLVLLLLLEDAGAQQGD
  GCGHTVLGPESGTLTSINYPQTYPNSTVCEWEIRVKMGERVRIKFGDFDIEDSDSCHFNYLRIYNGIGVS
  RTEIGKYCGLGLQMNHSIESKGNEITLLFMSGIHVSGRGFLASYSVIDKQDLITCLDTASNFLEPEFSKY
  CPAGCLLPFAEISGTIPHGYRDSSPLCMAGVHAGVVSNTLGGQISVVISKGIPYYESSLANNVTSVVGHL
  STSLFTFKTSGCYGTLGMESGVIADPQITASSVLEWTDHTGQENSWKPKKARLKKPGPPWAAFATDEYQW
  LQIDLNKEKKITGIITTGSTMVEHNYYVSAYRILYSDDGQKWTVYREPGVEQDKIFQGNKDYHQDVRNNF
  LPPIIARFIRVNPTQWQQKIAMKMELLGCQFIPKGRPPKLTQPPPPRNSNDLKNTTAPPKIAKGRAPKFT
  QPLQPRSSNEFPAQTEQTTASPDIRNTTVTPNVTKDVALAAVLVPVLVMVLTTLILILVCAWHWRNRKKK
  TEGTYDLPYWDRAGWWKGMKQFLPAKAVDHEETPVRYSSSEVNHLSPREVTTVLQADSAEYAQPLVGGIV
  GTLHQRSTFKPEEGKEAGYADLDPYNSPGQEVYHAYAEPLPITGPEYATPIIMDMSGHPTTSVGQPSTST
  FKATGNQPPPLVGTYNTLLSRTDSCSSAQAQYDTPKAGKPGLPAPDELVYQVPQSTQEVSGAGRDGECDV
  FKEIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080927
• ORF Size: 2325 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080927.1
• RefSeq Size: 6093 bp
• RefSeq ORF: 2328 bp
• Locus ID: 131566
• UniProt ID: Q96PD2
• Cytogenetics: 3
• Domains: F5_F8_type_C, CUB, LCCL
• Protein Families: Druggable Genome, Transmembrane
• MW: 84.9 kDa