ATP1B4 (NM_012069) Human Tagged ORF Clone

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SKU
RC224408
ATP1B4 (Myc-DDK-tagged)-Human ATPase, Na+/K+ transporting, beta 4 polypeptide (ATP1B4), transcript variant 2
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  $457.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ATP1B4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224408 representing NM_012069
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAAGGCAACTCCGGTCCAGAAGGGCTCCATCCTTTCCTTACAGTTATCGCTACAGACTCGATGATC
CGGATGAAGCGAACCAGAACTACTTAGCAGATGAAGAGGAGGAAGCAGAAGAAGAGGCTCGGGTGACGGT
GGTGCCCAAATCGGAGGAGGAGGAAGAAGAGGAGGAGAAAGAAGAGGAGGAAGAGGAGGAAAAGGAGGAG
GAAGAGGGTCAAGGTCAGCCAACAGGCAATGCCTGGTGGCAGAAATTGCAGATCATGAGTGAATACCTGT
GGGATCCAGAGAGAAGGATGTTTCTGGCCCGAACAGGCCTGATCTTACTCATTTACTTCTTCTTCTATGC
CTCCTTGGCTGCTGTGATCACCCTCTGCATGTACACACTATTTCTGACCATCAGTCCCTATATACCAACC
TTCACGGAGCGGGTAAAGCCTCCTGGAGTTATGATCAGACCCTTCGCCCATAGCCTTAACTTCAACTTCA
ACGTTTCTGAACCCGACACTTGGCAGCATTATGTGATTAGCCTAAATGGCTTTCTCCAGGGTTATAATGA
CAGTCTTCAAGAGGAAATGAATGTAGATTGTCCCCCGGGGCAGTACTTCATCCAAGATGGCAATGAGGAT
GAGGACAAGAAGGCCTGCCAATTTAAGCGCTCCTTCCTAAAGAACTGCTCTGGTCTGGAGGACCCAACTT
TTGGATACTCTACTGGACAGCCCTGCATCCTTCTAAAGATGAACCGGATTGTAGGCTTTCGTCCTGAGCT
TGGAGATCCTGTGAAGGTTTCCTGCAAAGTTCAGAGAGGTGATGAAAATGACATCCGATCCATCAGTTAC
TACCCAGAGTCGGCTTCTTTTGACCTCCGCTACTACCCTTACTACGGCAAACTGACTCACGTTAACTACA
CATCCCCCTTGGTGGCAATGCACTTTACAGACGTGGTGAAGAACCAAGCAGTGCCTGTGCAGTGCCAACT
GAAGGGCAAAGGCGTCATAAATGATGTCATCAATGATCGTTTTGTGGGCAGGGTAATCTTTACCCTGAAC
ATAGAAACT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC224408 representing NM_012069
Red=Cloning site Green=Tags(s)

MRRQLRSRRAPSFPYSYRYRLDDPDEANQNYLADEEEEAEEEARVTVVPKSEEEEEEEEKEEEEEEEKEE
EEGQGQPTGNAWWQKLQIMSEYLWDPERRMFLARTGLILLIYFFFYASLAAVITLCMYTLFLTISPYIPT
FTERVKPPGVMIRPFAHSLNFNFNVSEPDTWQHYVISLNGFLQGYNDSLQEEMNVDCPPGQYFIQDGNED
EDKKACQFKRSFLKNCSGLEDPTFGYSTGQPCILLKMNRIVGFRPELGDPVKVSCKVQRGDENDIRSISY
YPESASFDLRYYPYYGKLTHVNYTSPLVAMHFTDVVKNQAVPVQCQLKGKGVINDVINDRFVGRVIFTLN
IET

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012069
ORF Size 1059 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012069.5
RefSeq Size 3857 bp
RefSeq ORF 1062 bp
Locus ID 23439
UniProt ID Q9UN42
Cytogenetics Xq24
Protein Families Transmembrane
Protein Pathways Cardiac muscle contraction
MW 40.9 kDa
Summary This gene has been found in all vertebrate genomes sequenced to date. However, this gene has undergone a change in function in placental mammals compared to other species. Specifically, in fish, avian, and amphibian species, this gene encodes plasma membrane-bound beta-subunits of Na,K-ATPase. In placental mammals, the encoded protein interacts with the nuclear transcriptional coregulator SKIP and may be involved in the regulation of TGF-beta signaling. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Mar 2010
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Documents
Resources
"NM_012069" in other vectors (4)
SKU Description Size Price
RC224408L3 Lenti ORF clone of Human ATPase, Na+/K+ transporting, beta 4 polypeptide (ATP1B4), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC224408L4 Lenti ORF clone of Human ATPase, Na+/K+ transporting, beta 4 polypeptide (ATP1B4), transcript variant 2, mGFP tagged 10 ug
$757.00
RG224408 ATP1B4 (tGFP-tagged) - Human ATPase, Na+/K+ transporting, beta 4 polypeptide (ATP1B4), transcript variant 2 10 ug
$657.00
SC308949 ATP1B4 (untagged)-Human ATPase, Na+/K+ transporting, beta 4 polypeptide (ATP1B4), transcript variant 2 10 ug
$503.00
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