PTAR1 (NM_001099666) Human Tagged ORF Clone

CAT#: RC224206

PTAR1 (Myc-DDK-tagged)-Human protein prenyltransferase alpha subunit repeat containing 1 (PTAR1)

Product Images

Circular map for RC224206

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  Western blot validation of overexpression lysate (Cat# LY420480) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224206 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PTAR1 protein (Cat# TP324206). The protein was produced from HEK293T cells transfected with PTAR1 cDNA clone (Cat# RC224206) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PTAR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224206 representing NM_001099666
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGACCAGCGAGGAGGTGGCGGTGCTGGTGCAGCGGGTTGTGAAGGACATCACTAACGCCTTCA
  GGAGGAACCCACACATAGATGAAATTGGCCTGATCCCATGTCCTGAAGCTAGGTATAACCGGAGTCCCAT
  AGTCCTGGTTGAAAACAAACTGGGTGTGGAGAGCTGGTGTGTCAAGTTCCTTTTACCATATGTCCACAAC
  AAGCTCCTTTTGTACAGAACAAGAAAGCAATGGCTGAACAGAGATGAATTGATAGATGTCACATGTACCC
  TGCTGCTTCTAAACCCAGACTTTACCACTGCATGGAACGTGAGGAAAGAGCTGATCCTCTCTGGCACTTT
  AAATCCAATTAAGGATTTACATCTGGGAAAACTCGCCTTAACCAAGTTTCCAAAGAGTCCAGAAACATGG
  ATTCACAGGCGATGGGTGCTACAACAGCTAATTCAGGAAACCTCCTTGCCTTCCTTTGTGACCAAAGGAA
  ACTTGGGAACAATTCCCACAGAAAGGGCACAGCGACTCATACAAGAAGAGATGGAGGTCTGTGGTGAAGC
  AGCAGGGAGATACCCAAGCAACTATAATGCTTGGTCCCATCGCATCTGGGTTTTACAGCACTTGGCCAAG
  CTAGATGTCAAGATTCTTCTTGATGAACTATCTTCTACTAAACATTGGGCATCTATGCACGTTTCAGACC
  ACAGTGGATTTCACTACCGCCAGTTTTTGCTTAAGTCTTTGATTAGCCAAACTGTGATAGACAGTTCTGT
  GATGGAGCAAAATCCTTTGAGAAGTGAGCCAGCCCTAGTTCCTCCAAAAGATGAAGAAGCAGCAGTTTCA
  ACAGAAGAACCAAGGATTAATCTTCCCCATCTTCTAGAAGAAGAAGTTGAATTCAGCACTGATCTTATTG
  ATTCCTACCCAGGACATGAAACCCTTTGGTGTCATAGGCGGCATATTTTCTACCTTCAGCATCACTTAAA
  TGCAGGCTCCCAGCTGTCTCAAGCAATGGAAGTAGATGGACTGAATGACTCTAGCAAGCAAGGCTATTCC
  CAGGAAACCAAACGCCTGAAGCGGACGCCAGTTCCAGACTCCCTAGGCCTAGAAATGGAGCACAGGTTCA
  TTGATCAAGTATTGTCCACCTGTCGGAACGTGGAGCAAGCCAGGTTTGCCAGTGCATACAGGAAATGGCT
  GGTTACTTTGAGTCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224206 representing NM_001099666
  Red=Cloning site Green=Tags(s)
  
  MAETSEEVAVLVQRVVKDITNAFRRNPHIDEIGLIPCPEARYNRSPIVLVENKLGVESWCVKFLLPYVHN
  KLLLYRTRKQWLNRDELIDVTCTLLLLNPDFTTAWNVRKELILSGTLNPIKDLHLGKLALTKFPKSPETW
  IHRRWVLQQLIQETSLPSFVTKGNLGTIPTERAQRLIQEEMEVCGEAAGRYPSNYNAWSHRIWVLQHLAK
  LDVKILLDELSSTKHWASMHVSDHSGFHYRQFLLKSLISQTVIDSSVMEQNPLRSEPALVPPKDEEAAVS
  TEEPRINLPHLLEEEVEFSTDLIDSYPGHETLWCHRRHIFYLQHHLNAGSQLSQAMEVDGLNDSSKQGYS
  QETKRLKRTPVPDSLGLEMEHRFIDQVLSTCRNVEQARFASAYRKWLVTLSQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001099666
• ORF Size: 1206 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001099666.2
• RefSeq Size: 10057 bp
• RefSeq ORF: 1209 bp
• Locus ID: 375743
• UniProt ID: Q7Z6K3
• Cytogenetics: 9q21.12
• Protein Families: Druggable Genome
• MW: 46.2 kDa