SIGLEC14 (NM_001098612) Human Tagged ORF Clone

CAT#: RC224202

SIGLEC14 (Myc-DDK-tagged)-Human sialic acid binding Ig-like lectin 14 (SIGLEC14)

Product Images

Western blot validation of overexpression lysate (Cat# LY420640) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224202 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SIGLEC14 protein (Cat# TP324202). The protein was produced from HEK293T cells transfected with SIGLEC14 cDNA clone (Cat# RC224202) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SIGLEC14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224202 representing NM_001098612
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCCTGCTGCTGCTGCCCCTGCTGTGGGGGGGGTCCCTGCAGGAGAAGCCAGTGTACGAGCTGC
  AAGTGCAGAAGTCGGTGACGGTGCAGGAGGGCCTGTGCGTCCTTGTGCCCTGCTCCTTCTCTTACCCCTG
  GAGATCCTGGTATTCCTCTCCCCCACTCTACGTCTACTGGTTCCGGGACGGGGAGATCCCATACTACGCT
  GAGGTTGTGGCCACAAACAACCCAGACAGAAGAGTGAAGCCAGAGACCCAGGGCCGATTCCGCCTCCTTG
  GGGATGTCCAGAAGAAGAACTGCTCCCTGAGCATCGGAGATGCCAGAATGGAGGACACGGGAAGCTATTT
  CTTCCGCGTGGAGAGAGGAAGGGATGTAAAATATAGCTACCAACAGAATAAGCTGAACTTGGAGGTGACA
  GCCCTGATAGAGAAACCCGACATCCACTTTCTGGAGCCTCTGGAGTCCGGCCGCCCCACAAGGCTGAGCT
  GCAGCCTTCCAGGATCCTGTGAAGCGGGACCACCTCTCACATTCTCCTGGACGGGGAATGCCCTCAGCCC
  CCTGGACCCCGAGACCACCCGCTCCTCGGAGCTCACCCTCACCCCCAGGCCCGAGGACCATGGCACCAAC
  CTCACCTGTCAGGTGAAACGCCAAGGAGCTCAGGTGACCACGGAGAGAACTGTCCAGCTCAATGTCTCCT
  ATGCTCCACAGAACCTCGCCATCAGCATCTTCTTCAGAAATGGCACAGGCACAGCCCTGCGGATCCTGAG
  CAATGGCATGTCGGTGCCCATCCAGGAGGGCCAGTCCCTGTTCCTCGCCTGCACAGTTGACAGCAACCCC
  CCTGCCTCACTGAGCTGGTTCCGGGAGGGAAAAGCCCTCAATCCTTCCCAGACCTCAATGTCTGGGACCC
  TGGAGCTGCCTAACATAGGAGCTAGAGAGGGAGGGGAATTCACCTGCCGGGTTCAGCATCCGCTGGGCTC
  CCAGCACCTGTCCTTCATCCTTTCTGTGCAGAGAAGCTCCTCTTCCTGCATATGTGTAACTGAGAAACAG
  CAGGGCTCCTGGCCCCTCGTCCTCACCCTGATCAGGGGGGCTCTCATGGGGGCTGGCTTCCTCCTCACCT
  ATGGCCTCACCTGGATCTACTATACCAGGTGTGGAGGCCCCCAGCAGAGCAGGGCTGAGAGGCCTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224202 representing NM_001098612
  Red=Cloning site Green=Tags(s)
  
  MLPLLLLPLLWGGSLQEKPVYELQVQKSVTVQEGLCVLVPCSFSYPWRSWYSSPPLYVYWFRDGEIPYYA
  EVVATNNPDRRVKPETQGRFRLLGDVQKKNCSLSIGDARMEDTGSYFFRVERGRDVKYSYQQNKLNLEVT
  ALIEKPDIHFLEPLESGRPTRLSCSLPGSCEAGPPLTFSWTGNALSPLDPETTRSSELTLTPRPEDHGTN
  LTCQVKRQGAQVTTERTVQLNVSYAPQNLAISIFFRNGTGTALRILSNGMSVPIQEGQSLFLACTVDSNP
  PASLSWFREGKALNPSQTSMSGTLELPNIGAREGGEFTCRVQHPLGSQHLSFILSVQRSSSSCICVTEKQ
  QGSWPLVLTLIRGALMGAGFLLTYGLTWIYYTRCGGPQQSRAERPG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001098612
• ORF Size: 1188 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098612.2
• RefSeq Size: 2113 bp
• RefSeq ORF: 1191 bp
• Locus ID: 100049587
• UniProt ID: Q08ET2
• Cytogenetics: 19q13.41
• MW: 43.8 kDa
• Gene Summary: Putative adhesion molecule. Sialic acid-binding paired receptor which may activate associated receptors.[UniProtKB/Swiss-Prot Function]